Solid Phase Reversible Immobilization (SPRI) beads are a simple and effective reagent for DNA purification, but not for short oligo purification. The beads are paramagnetic particles coated with carboxyl groups that can reversibly bind to nucleic acid. However, SPRI beads can only purify DNA/RNA fragments that are 100 base pairs or longer. DNA/RNA fragments shorter than 100 base pairs are not effectively recovered.
Detail
Magnetic Beads (Short Oligo Purification)
Solid Phase Reversible Immobilization (SPRI) beads are a simple and effective reagent for DNA purification, but not for short oligo purification. The beads are paramagnetic particles coated with carboxyl groups that can reversibly bind to nucleic acid. However, SPRI beads can only purify DNA/RNA fragments that are 100 base pairs or longer. DNA/RNA fragments shorter than 100 base pairs are not effectively recovered.
Oligo purification can also be performed using spin column-based technology. The oligo size limitation for recovery is around 20 nt, as oligos under 20 nt have a very low recovery rate.
We have developed Magnetic Beads (Short Oligo Purification) Kit for short oligo purification. Our proprietary bead technology enables the recovery of oligos as short as 6 nt. 80% of the 6 nt oligos and 90% of the > 8 nt oligos can be recovered. The reagent also effectively removes impurities and unwanted components such as salts, proteins, dNTPs, detergents, and other contaminants. The magnetic bead reagents are RNase free, and can be used for both DNA and RNA applications.
Recovery rates of of short oligos. Oligos with different sizes were used as input. BioDynami Short Oligo Quantification Kit (Cat. # 40046) was used to quantify the recovery rates with modifications.
Features
Effective purification of short oligos
6 nt oligos: 80% recovery rate
>8 nt oligos: >90% recovery rate
Removal of impurities and unwanted reaction components
Other Products
P1006 HiPure Plasmid Plus 96 Kit
Product Info
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Product Info
Introduction
The HiPure Plasmid DNA Plus 96 Kit enables up to 96 minipreps to be performed simultaneously in less than 45 minutes on the Vacuum Manifold (Qiavac 96). This kit provides a fast, simple,and cost-effective plasmid DNA high-throughput method for routine molecular biology laboratory applications. HiPure Kits use silica membrane technology to eliminate the cumbersome steps associated with loose resins or slurries.Plasmid DNA purified with Kits is immediately ready for use. Phenol extraction and ethanol precipitation are not required, and high quality plasmid DNA is eluted in a small volume of Tris buffer or water.
Details
Specifications
Features
Specifications
Main Functions
Isolation up to 20µg plasmid DNA from 1.5ml bacterial culture using 96 well bind plate and 96 filterplate
Applications
Enzyme digestion, sequencing, PCR, labeling, etc.
Purification method
96 well plate
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
Conventional plasmid, plasmid less than 30KB
Sample amount
1-1.5ml（x96）
Yield
1-15µg/1ml
Elution volume
≥70μl
Time per run
≤60 minutes
Liquid carrying volume per column
800µl
Binding yield of column
70µg
Principle
The HiPure Plasmid procedure is based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt. The unique silica membrane used in the kit completely replaces glass or silica slurries for plasmid DNA minipreps. The procedure consists of 3 basic steps: Preparation and clearing of a bacterial lysate by alkaline method，then transfer the supernatant to column to bind DNA. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
Advantages
High purity – purified plasmid can be directly used in sequencing, enzyme digestion and PCR, etc.
Fast – it takes only 60 minutes to complete the isolation
High yield – up to 1mg plasmid can be binded in one column
Economy – high cost performance
Kit Contents
Contents
P100601
P100602
P100603
Purification Times
1 x 96 Preps
4 x 96 Preps
20 x 96 Preps
RNase A
5 mg
20 mg
100 mg
Buffer P1
30 ml
120 ml
600 ml
Buffer P2
30 ml
120 ml
600 ml
Buffer P3
40 ml
180 ml
800 ml
Buffer PW1
100 ml
500 ml
2 x 1000 ml
Buffer PW2
50 ml
2 x 100 ml
4 x 200 ml
Elution Buffer
150 ml
60 ml
300 ml
Lysate Clear Plate
1
4
20
HiPure DNA Plate
1
4
20
1.6 ml Collection Plate
1
4
20
0.5ml Elute Plate
1
4
20
Storage and Stability
The kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve. After addition of RNase A，Buffer P1 is stable for 6 months when stored at 2-8°C.
The HiPure Plasmid DNA Plus 96 Kit enables up to 96 minipreps to be performed simultaneously in less than 45 minutes on the Vacuum Manifold (Qiavac 96). This kit provides a fast, simple,and cost-effective plasmid DNA high-throughput method for routine molecular biology laboratory applications. HiPure Kits use silica membrane technology to eliminate the cumbersome steps associated with loose resins or slurries.Plasmid DNA purified with Kits is immediately ready for use. Phenol extraction and ethanol precipitation are not required, and high quality plasmid DNA is eluted in a small volume of Tris buffer or water.
Biotin-PEG3-alkyne is a biotin PEG reagent with an alkyne group that enables click reaction with azido molecules in the presence of Cu catalyst. The hydrophilic PEG spacer arm imparts water solubility that is transferred to the biotinylated molecule.
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Biotin-PEG3-alkyne is a biotin PEG reagent with an alkyne group that enables click reaction with azido molecules in the presence of Cu catalyst. The hydrophilic PEG spacer arm imparts water solubility that is transferred to the biotinylated molecule.
