NGS Library Quantification Standards With PCR Primers
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The NGS Library Quantification Standards with PCR Primers (for illumina platform) were developed for quantification of the NGS library concentration for illumina sequencing platform. It is comprised of Library Standards (six 10-fold dilutions) and a primer mix.
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The NGS Library Quantification Standards with PCR Primers (for illumina platform) were developed for quantification of the NGS library concentration for illumina sequencing platform. It is comprised of Library Standards (six 10-fold dilutions) and a primer mix.
Quantification of the NGS library of the amplifiable molecules is critical for the quality of the sequencing data. Adequate library concentration will maximize sequencing capacity. Poor library concentration results in either low or high cluster density on the flow cell, which can lead to low sequencing capacity.
It is not accurate to measure the concentration of NGS library with standard DNA quantification methods such as spectrophotometer or fluorometer. QPCR is the best way for library quantification with high consistency and reproducibility of library quantitation.
BioDynami Library Quantification Standards with PCR Primers (for illumina platform): Amplification curve of 6 standards.
Our reagent is a highly sensitive, Real time PCR-based quantification that specifically designed for NGS libraries using illumina sequencing platform. The amplification uses illumina adaptor sequences as primers, and only the fully adaptor-ligated libraries will be amplified. Therefore the reagent provides an accurate estimation of the library concentration based on true sequenceable illumina libraries. In addition, this kit can also be used for confirmation of ligation reaction after completion of library preparation.
Our library quantification standards is compatible with commercial SYBR Green based QPCR reagents. This makes it more flexible for scientists who want to use their real time PCR reagent. Quantification of library concentration is achieved by comparison with a standard curve generated from the Library Standards.
Attogene’s Cadmium Lateral Flow Kit can be used to detect Cadmium in water samples at 5 ppb in the field.
Format: 10 test cassettes
Run Time: 15-30 minutes
Cadmium contamination is a serious worldwide environmental problem. As it is difficult to detoxify by chemical or biological methods, gradual Cadmium accumulation in the nervous and cardiovascular systems of the human body can subsequently cause serious diseases. Long-term health consequences of drinking cadmium-contaminated food and water include numerous negative health effects. Attogene’s Cadmium Lateral Flow test gives results conforming of 5ppb or greater. Using the supplied pipette, simply fill the vial with your water sample, place the water into the sample port and wait 15-30 minutes.
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Format: 10 test cassettes
Run Time: 15-30 minutes
Detection: Cadmium in water samples at 5 ppb
Fractionate leukocytes from whole blood in minutes with provided RBC Lysis Buffer
Isolate total RNA, including microRNA, without phenol
Rapid and convenient spin-column format and 96-well plate for high throughput applications
Purified RNA is ready for any downstream application including RT-PCR, qRT-PCR, NGS, arrays and more. Find out more information on Norgen’s NGS services
Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
These kits provide a rapid method for the isolation and purification of total leukocyte (white blood cell) RNA from mammalian blood samples. These kits are supplied with an RBC (red blood cell) Lysis Buffer for selective removal of red blood cells and fractionation of leukocytes by centrifugation. Isolation of leukocyte RNA results in improved expression profiling and other downstream applications by removing the masking effects of some RNAs which are very abundant in whole blood, such as globin mRNAs. These kits are able to isolate total leukocyte RNA, including both large mRNA and all small RNA species containing microRNA (miRNA) and small silencing RNA (siRNA). The purified RNA is of the highest quality and can be used in a number of downstream applications.
Leukocyte RNA Purification Kit (Spin Column)
This kit provides a rapid method for the isolation and purification of total leukocyte RNA from mammalian blood samples in 40 minutes. Allowable blood input ranges from 10 μL to 2 mL or 3 x 106 Leukocytes
Leukocyte RNA Purification Plus Kit (Plus)
Norgen’s Leukocyte RNA Purification Plus Kit provides a rapid method for the isolation and purification of total leukocyte (white blood cell) RNA from up to 3 mL of mammalian blood samples. Complete 10 purifications in as little as 40 minutes.
Purification is based on 96-well column chromatography using Norgen’s proprietary resin as the separation matrix. Purification can be performed using either a vacuum manifold or centrifugation. Norgen’s kit allows for the isolation of total leukocyte RNA, including all small RNA species. The purified RNA is of the highest quality and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, northern blotting, RNase protection and primer extension, and expression array assays. Allowable blood input ranges from 10 μL to 1 mL or 1.5 x 106 Leukocytes.
*Additional RBC Lysis Solution is required for input Volumes >150 µL
Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. The RBC Lysis Buffer should be stored at 4°C upon arrival. These reagents should remain stable for at least 1 year in their unopened containers.
