Screening of Microcystins in water samples at 0.1 ppb (drinking water) Format: 10 tests (5 tests/5 controls) Not provided: Water Sample Bottles Run Time: 15 Minutes Finished Drinking Water
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Screening of Microcystins in water samples at 0.1 ppb (drinking water)
Attogene’s Microcystin Test Kit (Rapid – Drinking Water) can be used to detect microcystins in water to as low as 0.1ppb; highly sensitive, rapid, robust screening kit for microcystins and nodularins.
The most frequently reported cyanobacterial toxins are the hepatotoxic microcystins (MCs). MCs are peptides with a molecular weight ranging from 900 to 1,100 Da. They consist of seven amino acids of which the two terminal amino acids of the linear peptide are condensed to form a cyclic compound.
A tiered notification system which takes different actions based on thresholds for microcytin-LR concentrations in drinking waters has been developed. This is guidance that allows states to take various actions.
For the rapid screening of microcystins in drinking water samples at or above 0.1 ppb. Samples requiring regulatory action should be confirmed by ELISA, HPLC, or other conventional methods.
To protect consumers from adverse health effects caused by these toxins, the World Health Organization (WHO) has proposed a provisional upper limit for Microcystin-LR of 1.0 ppb (μg/L) in drinking water.
The U.S. Environmental Protection Agency (EPA) has also established guidelines for Microcystins in drinking water:
2,5-Dioxopyrrolidin-1-yl 2-(prop-2-yn-1-yloxy)acetate is a Click Chemistry reagent with a propargyl group and an NHS ester group. The propargyl group can react with biomolecules containing azide group via copper catalyzed Click Chemistry reaction. The NHS ester is an amine reactive group which can be used for derivatizing peptides, antibodies, amine coated surfaces, etc. Reagent grade, for research use only.
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2,5-Dioxopyrrolidin-1-yl 2-(prop-2-yn-1-yloxy)acetate is a Click Chemistry reagent with a propargyl group and an NHS ester group. The propargyl group can react with biomolecules containing azide group via copper catalyzed Click Chemistry reaction. The NHS ester is an amine reactive group which can be used for derivatizing peptides, antibodies, amine coated surfaces, etc. Reagent grade, for research use only.
Rapid isolation of both small and large species of DNA from urine
Convenient spin column format
Effective removal of PCR inhibitors
Purified DNA is highly suited to sensitive downstream applications
Allows for the purification of viral DNA from urine
Both high molecular weight DNA (greater than 1 kb in size; mostly cell associated) and the smaller DNA (150 – 250 bp; derived from the circulation) is effectively isolated and purified using a rapid and convenient spin column protocol. This kit can be used to isolate DNA from a broad range of viruses in urine as well. Salts, metabolic wastes, proteins and other contaminants are removed to yield inhibitor-free DNA for use in sensitive applications. The DNA is of excellent quality for various downstream applications such as PCR, qPCR and DNA fingerprinting, methylation studies and more.
This kit provides a fast, reliable and simple procedure for isolating DNA from urine volumes ranging from 50 μL to 1.75 mL of urine. Multiple samples can be processed in 30 minutes.
Urine DNA Isolation Kit (Slurry Format)
This kit provides a fast, reliable and simple procedure for isolating DNA from urine volumes ranging from 3 mL to 25 mL. Multiple samples can be processed in 30 minutes. Multiple samples can be processed in 45 minutes.
Urine DNA Isolation Maxi Kit (Slurry Format)
This kit provides a fast, reliable and simple procedure for isolating DNA from urine volumes ranging from 25 mL of urine up to 80 mL. Multiple samples can be processed in 45 minutes.
Background
DNA found in urine can be divided into 2 basic categories. The larger species, genomic-DNA (gDNA), is generally greater than 1 kb in size, and appears to be derived mainly from exfoliated cells. The second species is smaller, generally between 150 and 250 bp (apoptotic-DNA), and derives, at least in part, from the circulation. The second species is also considered as an RNA/DNA hybrid as reported by Halicka et al. (2000). Both types of DNA can be isolated reliably using this kit.
Storage Conditions and Product Stability All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm up Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.
Plasma/Serum Exosome and Free-Circulating RNA Isolation Kits
Product Info
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Product Info
Overview
Isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias.
Versatile sample input ranges
Isolate all sizes of free-circulating RNA, including microRNA
The purified exosomal RNA is free from any circulating RNA-binding proteins
No phenol extractions, Proteinase K treatment, nor carrier RNA
No time-consuming ultracentrifugation, filtration nor special syringes are required
No precipitation reagents, nor overnight incubation required
Concentrate isolated exosomal RNA and are free-circulating RNA into a flexible elution volume ranging from 50 µL to 100 µL
Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
These kits provide a fast, reliable and convenient method to sequentially isolate and concentrate exosomal RNA as well as Free-Circulating RNA from different plasma/serum sample volumes. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. These kits are designed to isolate all sizes of RNA, including microRNA as well as all sizes of the free-circulating protein-bound RNA, including microRNA. These kits provide a clear advantage over other available kits in that they do not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, these kits allow the user to elute into a flexible elution volume ranging from 50 µL to 100 µL. The RNA isolated from the purified exosomes is free from any protein-bound circulating RNA and is of the highest integrity. Moreover, the free-circulating, protein-bound, RNA is free from any exosomal RNA. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.
Plasma/Serum Exosome and Free-Circulating RNA Isolation Mini Kit
For sample volumes ranging from 50 µL to 1 mL.
Plasma/Serum Exosome and Free-Circulating RNA Isolation Midi Kit
For sample volumes ranging from 1 mL to 4 mL.
Plasma/Serum Exosome and Free-Circulating RNA Isolation Maxi Kit
All sizes, including miRNA and small RNA (< 200 nt)
Elution Volume
50 – 100 µL
Time to Complete 10 Purifications
40 – 45 minutes
Average Yields*
Variable depending on specimen
*Please check page 5 of the product insert for the average yields and the common RNA quantification methods
Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.
Important Note This kit is suitable for the purification of exosomes from fresh or frozen serum or plasma prepared from blood collected on either EDTA or Citrate. Plasma samples prepared from blood collected on heparin should not be used as heparin can significantly interfere with many downstream applications such as RT-PCR.
