Description
- • Appearance: White/Pink with lens
- • Length of strip: 86mm
- • Width of strip: 6mm
- • Length of device: 12mm
- Width of device: 15mm
500 Lateral Flow Cassettes (top and bottom)
Cassette Lettering: CT
Fits strips that are 86 mm x 6 mm
Perfect for development and product launch
Large quantities available
This kit provides a convenient and rapid method to purify total RNA from small amounts of stool samples. All types of stool samples can be processed with this kit, including animal fecal samples, manure and samples collected using Norgen’s Stool Nucleic Acid Collection and Preservation Tubes (Cat. 45660). The kit removes all traces of humic acids using rapid and simple spin column procedures. Bead tubes are also provided for effective homogenization of stool. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA and small interfering RNA. Both host and microbial RNA is recovered. The protocol does not rely on the use of phenol or chloroform, thereby providing a user friendly procedure and allowing high-throughput analysis. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR and reverse transcription PCR for gene expression analysis. The procedure can be completed in approximately 30 minutes for 10 samples.
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| Kit Specifications | |
| Maximum Stool Input | 200 mg (fresh/frozen stool) or 400 μL (preserved stool) |
| Type of Stool Processed | Preserved, fresh, and frozen stool from humans and animals |
| Maximum Column Binding Capacity | 50 μg |
| Maximum Column Loading Volume | 600 μL |
| Time to Complete 10 Purifications | 30 minutes |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
| Component | Cat. 49500 (50 preps) |
|---|---|
| Lysis Buffer C | 60 mL |
| Wash Solution A | 38 mL |
| Elution Buffer E | 6 mL |
| Bead Tubes | 50 |
| Spin Columns | 50 |
| Collection Tubes | 50 |
| Elution Tubes (1.7 mL) | 50 |
| Product Insert | 1 |
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit. Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
Primer and probe mix (150 reactions)
Reverse Transcription, target specific primers (RNA genome viruses only)
Copy number standard curve (sufficient for multiple standard curves)
Internal extraction control – Read through VIC channel*
Endogenous control (150 tests)
RNAse/DNAse free water
*alternative fluorophores available on request
DBCO-PEG4-PFP ester is a PFP active ester containing a DBCO group which can undergo copper-free Click Chemistry with azide (N3). The PFP ester moiety is reactive with amine groups. PFP esters have been are stable compounds and are less susceptible to undergo hydrolysis. The hydrophilic PEG linker increases the water solubility of a compound in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
DBCO-PEG4-PFP ester is a PFP active ester containing a DBCO group which can undergo copper-free Click Chemistry with azide (N3). The PFP ester moiety is reactive with amine groups. PFP esters have been are stable compounds and are less susceptible to undergo hydrolysis. The hydrophilic PEG linker increases the water solubility of a compound in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.