CO00021 Midstream Cassette 126x15mm, pink

TGM12 Table top capillary vessel Haematocrit centrifuge

Application:

Measure the ratio of blood corpuscle and separate microblood and micro solution.

Model: TGM12 Table top high speed capillary vessel centrifuge

Features:

Brushless DC motor, free maintenance, no powder pollution, micro computer control, digital display indicates the time、speed and RCF. Electric lid lock、 over speed and imbalance protection. The centrifuge body is made of high quality steel, Safe and reliable.

Technical parameters:

Max speed	12000r/min	Max RCF	15800×g
Time range	1~99min	Speed accuracy	±20r/min
capacity	24pieces capillary vessel	Noise	≦55dBA
Weight	13KG	Dimension	355×270×205mm
Packaged weight	15KG	Packaged dimension	455×335×270mm
Power	AC 220V 50HZ 5A	Motor	Brushless
Package	Carton box

The kit was developed for construction of high quality libraries for next generation sequencing (illumina and MGI Platforms). The kit needs double strand DNA fragments (blunt and/or sticky) as input DNA for NGS library preparation, and is compatible with DNA fragments generated from both enzymatic methods and physical methods (sonication, nebulization etc.). Library multiplexing is possible with different types of indexes.

The kit was optimized for next generation sequencing (NGS) library preparation with different types of samples. Most of DNA library preparation requires the ligation of sheared DNA fragments to library adaptors and the DNA library preparation is closely related to the quality of NGS data. With BioDynami’s unique DNA library preparation technologies, the fast and simple kit allows high quality NGS library preparation to be completed in 1.5 hours with only 10 minutes of hands-on time.

<img decoding="async" src="https://biodynami.com/wp-content/uploads/2020/07/NGS-DNA-Library-Prep-Kit-illumina-1.png" alt=""

Some genomic regions are very difficult to be covered evenly and usually result in very low coverage rate or gap in these regions.

Typical difficult regions are:
• with high GC contents
• have secondary structures: mainly due to repeat sequences
• the worst cases: have both high GC contents and repeated sequences.

Example: human TERT gene is one of the most difficult regions as shown above. NGS data showed that BioDynami kit has the best performance to cover the extremely difficult human TERT gene region.

Limited GC bias across whole genome

<img decoding="async" src="https://biodynami.com/wp-content/uploads/2020/07/BioDynami-NGS-DNA-Library-Prep-Kit-GC-bias.png" alt="

Three index types are available for the illumina platform kits:

Non-index (illumina Cat.# 30009): Libraries do not have index.

Index (illumina Cat.# 30021): A unique barcode sequence with 6 bases has been included in each of the index primers. RNA Sequencing library multiplexing is possible with up to 48 samples. Index information can be downloaded here.

Unique dual index (illumina Cat.# 30023): RNA Sequencing library multiplexing up to 96 samples is possible with the unique dual indexes. We have developed a 4-Base Difference Index System. The system can generate indexes with at least 4 bases different from others in the 8-base indexing region. the unique dual indexing primers identify sequencing errors such as index hopping, mis-assignment, and de-multiplexing errors. Index information can be downloaded here.

Indexes are available for the MGI platform kits (Cat.# 34021).

The kit was developed for construction of high quality libraries for next generation sequencing (illumina and MGI Platforms). The kit needs double strand DNA fragments (blunt and/or sticky) as input DNA for NGS library preparation, and is compatible with DNA fragments generated from both enzymatic methods and physical methods (sonication, nebulization etc.). Library multiplexing is possible with different types of indexes.

Propargyl-PEG4-alcohol is a Click Chemistry reagent that can react with azide-bearing compounds or biomolecules; copper is needed as a catalyst. The PEG units help increase the solubility of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Propargyl-PEG4-alcohol is a Click Chemistry reagent that can react with azide-bearing compounds or biomolecules; copper is needed as a catalyst. The PEG units help increase the solubility of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.