CO00019 Midstream Cassette 140x15mm, 86×4.5mm blue w/lens

Tri-(Propargyl-PEG2-ethoxymethyl)-methane-amido-PEG3-carboxylate is reactive with azide-bearing compounds or biomolecules via copper catalyzed azide-alkyne Click Chemistry to yield a stable triazole linkage. The terminal carboxylic acid can react with primary amino groups in the presence of activators (e.g. EDC, or HATU) to form a stable amide bond. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Tri-(Propargyl-PEG2-ethoxymethyl)-methane-amido-PEG3-carboxylate is reactive with azide-bearing compounds or biomolecules via copper catalyzed azide-alkyne Click Chemistry to yield a stable triazole linkage. The terminal carboxylic acid can react with primary amino groups in the presence of activators (e.g. EDC, or HATU) to form a stable amide bond. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Description 

ExcelRT™ Reverse Transcription Kit is a complete, efficient and convenient kit to synthesize high quality first strand cDNA. This kit contains ExcelRT™ Reverse Transcriptase, which is able to synthesize the first strand cDNA at 37~50°C. The ExcelRT™ Reverse Transcriptase is a recombinant Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase, which is designed to reduce RNase H activity and create better thermal stability. This kit also contains RNAok™ RNase Inhibitor, which is active against RNase A, RNase B, and RNase C. This product is supplied with oligo (dT)₂₀ and random hexamers, which are used to synthesize cDNA from poly(A) tailed mRNA and total RNA, respectively. 

Features

• Contains all components for reverse transcription 
• High yield 
• Thermostable, up to 50°C, during first strand synthesis 
• High processivity, generating cDNA up to 8 kb 
• Reduced RNase H ribonuclease activity 

Application

• Generation of first strand cDNA from total RNA or mRNA.
• Suitable for generating cDNA from RNA with strong secondary structure which can be reduced at temperature up to 50°C.

Storage

-20°C for 24 months 

High yield

High sensitivity

Thermostable, up to 50°C, during first strand synthesis

 

Contents

ComponentVolume    ExcelRT™ Reverse Transcriptase (200 U/μl) 100 μl      RNase Inhibitor (20 U/μl)100 μl      5X RT Buffer (DTT)500 μl      dNTPs Mix (10 mM each)200 μl      Oligo (dT)20 (50 μM)100 μl      Random Hexamers (100 μM)100 μl      DEPC-Treated H2O  1 ml x 2

Storage Buffer

Reverse Transcriptase: 20 mM Tris-HCl (pH 7.5), 200 mM NaCl, 0.1 mM EDTA, 1 mM DTT, stabilizer and 50% (v/v) glycerol

RNase Inhibitor: 40 mM HEPES-KOH (pH 7.5), 100 mM KCl, 8 mM DTT, 0.1 mM EDTA, stabilizer and 50% (v/v) glycerol

5X RT buffer (DTT)

250 mM Tris-HCl (pH 8.3), 375 mM KCl, 15 mM MgCl₂ and 50 mM DTT

Storage

-20°C for 24 months 

ExcelRT™ Reverse Transcription Kit is a complete, efficient and convenient kit to synthesize high quality first strand cDNA. This kit contains ExcelRT™ Reverse Transcriptase, which is able to synthesize the first strand cDNA at 37~50°C. The ExcelRT™ Reverse Transcriptase is a recombinant Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase, which is designed to reduce RNase H activity and create better thermal stability. This kit also contains RNAok™ RNase Inhibitor, which is active against RNase A, RNase B, and RNase C. This product is supplied with oligo (dT)20 and random hexamers, which are used to synthesize cDNA from poly(A) tailed mRNA and total RNA, respectively.

K-GLUT

SKU: 700004301

60 assays (manual) / 600 assays (microplate) / 700 assays (auto-analyser)

Content:	60 assays (manual) / 600 assays (microplate) / 700 assays (auto-analyser)
Shipping Temperature:	Ambient
Storage Temperature:	Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:	> 1 year under recommended storage conditions

Analyte:	L-Glutamic Acid, MSG
Assay Format:	Spectrophotometer, Microplate, Auto-analyser
Detection Method:	Absorbance
Wavelength (nm):	492
Signal Response:	Increase
Linear Range:	0.4 to 20 µg of L-glutamic acid per assay
Limit of Detection:	0.21 mg/L
Reaction Time (min):	~ 8 min
Application examples:	Fruit and vegetables (e.g. tomato), processed fruit and vegetables (e.g. tomato puree / juice, ketchup, soy sauce), condiments, processed meat products (e.g. extracts, bouillon and sausages), soup, pharmaceuticals and other materials (e.g. biological cultures, samples, etc.).
Method recognition:	Methods based on this principle have been accepted by ISO, GOST and NMKL

The L-Glutamic Acid test kit is a simple, reliable, rapid and accurate method for the measurement and analysis of L-glutamate (MSG) in foodstuffs.

Note for Content: The number of manual tests per kit can be doubled if all volumes are halved.  This can be readily accommodated using the MegaQuant^TM  Wave Spectrophotometer (D-MQWAVE).

Explore more organic acid test kits.

Advantages

• Very competitive price (cost per test) 
• All reagents stable for > 2 years after preparation 
• Glutamate dehydrogenase solution stable below -10^oC  
• No wasted diaphorase solution (stable suspension supplied)  
• Rapid reaction 
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing 
• Standard included 
• Suitable for manual, microplate and auto-analyser formats

The L-Glutamic Acid test kit is a simple, reliable, rapid and accurate method for the measurement and analysis of L-glutamate (MSG) in foodstuffs.