Exceptional value for money
Rapid detection of all clinically relevant subtypes
Positive copy number standard curve for quantification
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target
Accurate controls to confirm findings
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit.
Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
Other Products
Escherichia coli (E.coli) O157:H7 Quantified Bacterial DNA Standards
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Quantified standard to be used as a positive control or PCR quantification standard
Vigorously quantified using multiple methods
Includes partial sequence of STX-1 subunit A, and STX-2 subunit A genes (please refer to product insert for more details)
Compatible with E. coli O157:H7 TaqMan PCR Detection Kit and E. coli O157:H7 TaqMan PCR Detection Probe/Primer and Control Set
E. coli O157:H7 is a rod-shaped, gram negative bacterium. It is an enterohemorrhagic strain of the common E. coli bacterium and infection by the O157:H7 strain is commonly associated with hemorrhagic colitis. E. coli O157:H7 is recognized by its somatic (cell wall) antigen (O157) and its flagella antigen (H7). In addition, E. coli O157:H7 is known to produce Shiga-like toxins, which cause severe symptoms. While most patients can recover from the infection, up to 15% of the patients may develop hemolytic uremic syndrome, a type of kidney failure that could be fatal. Infection of E. coli O157:H7 usually results from consumption of poorly prepared food including undercooked meat (particularly ground beef), untreated water or raw unpasteurized milk.
Norgen’s E. coli O157:H7 Quantified Bacterial DNA Standards are cloned fragments of the two Shiga-like toxin regions purified using Norgen’s sample preparation technology. They include partial sequences of STX-1 subunit A gene and STX-2 subunit A gene. Please refer to Appendix A for sequence information. The purified DNA is quantified vigorously using multiple methods including spectrophotometry, gel densitometry and real-time PCR. It is intended to be used as positive controls or PCR quantification standards for E. coli O157:H7. This product is compatible with Norgen Biotek’s E. coli O157:H7 Taqman PCR Detection Kit (Cat. TM41350) and E. coli O157:H7 Taqman PCR Detection Probe/Primer and Control Set (Cat. TM41310).
Storage Conditions Upon receipt, store Norgen’s E. coli O157:H7 Quantified Bacterial DNA Standards at -20°C or lower. Avoid multiple freeze-thaw cycles. If needed, prepare smaller working aliquots and store at -20°C or lower.
CE-IVD marked version available for in vitro diagnostic use
Available in TaqMan format for analysis
Epstein-Barr Virus (EBV) is a member of the Herpes family of virus and is one of the most common viruses in humans. The virus occurs globally and causes infectious mononucleosis. Most individuals become infected with EBV and develop adaptive immunity, with the majority of adults between the ages of 35 and 40 having been infected. The virus has been implicated as having a primary role in multiple autoimmune diseases, several lymphoproliferative disorders and cancers, particularly Hodgkin’s disease and Burkitt’s lymphoma. Many children become infected with EBV once maternal antibody protection disappears, however these infections usually do not result in symptom development. During adolescence the virus will cause mononucleosis in up to 69% of infections
EBV TaqMan PCR Kit, 100 reactions
Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
Specific Primer and Probe mix for the pathogen/virus/viroid of interest
Primer and Probe mix
Positive and negative control to confirm the integrity of the kit reagents
EBV TaqMan PCR Probe/Primer Set and Controls, 100 reactions
Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
Nuclease-free water
Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix
Storage Conditions and Product Stability All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
TGL18 Tabletop 21000rpm High Speed large capacity Swing out rotor 4x800ml universal Refrigerated Centrifuge Adapters for 5/10/15/50ml tubes
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TGL18 Tabletop 21000rpm High Speed large capacity Swing out rotor 4x800ml universal Refrigerated Centrifuge Adapters for 5/10/15/50ml tubes. And holding Angle Rotors like 24×1.5/2ml, 6x50ml, 12x15ml…in high speed separation smooth.
Holding lots of Swing Out Rotors & Angle Rotors for options. Mainly used in animal and plant molecular biology, cell biology, food safety, life science, agriculture and forest science, animal husbandry science, biological products, pharmaceutical products and other fields of ideal equipment for the separation and precipitation of samples preparation.
TGL18 Universal Refrigerated Centrifuge Features:
1. With silent-block and shock absorbers that guarantees smooth and quiet operations & fast cooling.
2. The centrifuge body is made of high-quality steel, safe and reliable.
3. Microprocessor digital control of all functions:speed, time,
temperature, acceleration/deceleration, RCF, 10 program memory, operation fault display.
4. Electronic safety lock alarm inside, just press down the top lid until it will lock in sealed and open up after separation automatically.
5. Easy open the centrifuge lid via pneumatic springs; the operation area of the centrifuge is at the front end.
6. RPM/RCF adjustable along with the speed setting calculating automatically.
7. 10 selective acceleration and deceleration rates for options
8. The self-diagnostic system provides a protection for imbalance over speed/voltage and electronic lock fault.
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Metal housing with 3-tiers protection. It can hold both swing out rotors and angle rotors in low speed and high speed spining to switch smoothly. And its Quiet noise, Less vibration & Fast cooling guarantee a perfect separation.
