Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit. Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
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R6641 MagPure Plant RNA Kit
Product Info
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Product Info
Introduction
This product supplies a simple and rapid extraction of total RNA from plant and fungal samples. The kit is based on super paramagnetic particles purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction process takes only 60 minutes. Purified RNA is ready for downstream applications such as RT-PCR, virus RNA testing and so on. MagPure RNA Kits buffers can be used for both manual extraction process and automatic nucleic acid extraction machines. This Kits is suitable for extracting RNA from ≤5×106 cultured cells, 20mg tissue and <50mg plant samples.
Details
Specifications
Features
Specifications
Main Functions
Isolation total RNA from 50mg plant using magnetic particles
Applications
RT-PCR, cDNA synthesis, second generation sequencing
Purification method
Polydisperse magnetic beads
Purification technology
Magnetic beads technology
Process method
Manual or automatic
Sample type
Plant and fungus samples
Sample amount
≤50mg
Yield
2-100μg
Time per run
≤60 minutes
Principle
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. After adding magnetic particles and binding solution, RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally RNA was eluted by Elution Buffer.
Advantages
High quality – high purity total RNA can be directly used in various sensitive downstream applications
Safe – no phenol chloroform extraction required
Fast – several samples can be extracted in 60 minutes by column method
Universal – two lysates suitable for most plant or fungal tissue samples
Kit Contents
Contents
R664101
R664102
R664103
Purification Times
48 Preps
96 Preps
480 Preps
MagPure RNA Particles
1.7 ml
3.5 ml
18 ml
DNase I
600 μl
2 x 600 μl
10 x 600 μl
DNase Buffer
30 ml
40 ml
200 ml
Buffer PRC1
40 ml
70 ml
350 ml
Buffer RL
40 ml
70 ml
350 ml
Buffer MCB*
18 ml
30 ml
150 ml
Buffer MW1*
22 ml
44 ml
220 ml
Buffer MW2*
20 ml
50 ml
2 x 100 ml
RNase Free Water
10 ml
15 ml
120 ml
Storage and Stability
MagPure RNA Particles should be stored at 2–8°C upon arrival. DNase I should be stored at -20°C. However, short-term storage (DNase I up to 1 weeks, MagPure RNA Particles up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under theseconditions.
Document
This product supplies a simple and rapid extraction of total RNA from plant and fungal samples. The kit is based on super paramagnetic particles purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction process takes only 60 minutes. Purified RNA is ready for downstream applications such as RT-PCR, virus RNA testing and so on. MagPure RNA Kits buffers can be used for both manual extraction process and automatic nucleic acid extraction machines. This Kits is suitable for extracting RNA from ≤5×106 cultured cells, 20mg tissue and
Robust lysis buffer is well-suited to even challenging samples such as pine needle, grape leaf, etc
Isolate total RNA (including microRNA) without phenol
Isolated RNA is of high quality, integrity and diversity
Also available in 96-well format for high throughput applications
Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
Norgen’s Plant/Fungi Total RNA Purification Kit provides a rapid method for the isolation and purification of total RNA, including virus and viroid RNA, from a wide range of plants. Total RNA can be purified from fresh or frozen plant tissues, plant cells or filamentous fungi samples using this kit. All sizes of RNA are purified, including microRNA (miRNA) . The procedure is rapid and convenient.
The RNA is purified without the use of phenol or chloroform. The purified RNA is of the highest quality, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.
Norgen’s Plant/Fungi Total RNA Purification Kit is also available in a 96-well (High Throughput) format for high throughput applications. Purification with the 96-well plates can be performed using either a vacuum manifold or centrifugation.
* Yield will vary depending on the type of sample processed.
* Yield will vary depending on the type of sample processed.
Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
Select Plants and Fungi Tested with the Norgen Plant/Fungi Total RNA Purification Kits
The PCR Decontamination Kit can remove contaminating DNA in PCR master mixes, without reduction of PCR sensitivity.
The double-strand specific property of the dsDNase allows decontamination with primers and probe present.
Efficient for end-point PCR, probe-based qPCR, and some SYBR based qPCR mixes.
Contaminating bacterial DNA can be reduced to levels below the detection limit.
Fast and easy protocol.
Flat NTCs (No Template Controls).
Decontamination of master mixes without reduction of sensitivity has always been a challenge. Especially when minor amounts of DNA are targeted, contaminating DNA is a major problem. Any loss of sensitivity in the qPCR assay caused by the decontamination protocol is unacceptable.
In Figure 1, it is demonstrated that the PCR decontamination kit can remove contaminating DNA from a qPCR mix to non-detectable levels (flat NTC), without affecting the sensitivity of the qPCR.
Kit Contents
DTT (Inactivation Aid)
dsDNase
Document
The PCR Decontamination Kit can remove contaminating DNA in PCR master mixes, without reduction of PCR sensitivity.
