Primer and probe mix (150 reactions)
Reverse Transcription, target specific primers (RNA genome viruses only)
Copy number standard curve (sufficient for multiple standard curves)
Internal extraction control – Read through VIC channel*
Endogenous control (150 tests)
RNAse/DNAse free water
*alternative fluorophores available on request
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit. Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
Propargyl-PEG5-t-butyl ester has one of the functional groups protected by t-butyl group. The protection can be removed under acidic conditions. The propargyl group forms triazole linkages with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry. The PEG units help improving the water-solubility of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
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Propargyl-PEG5-t-butyl ester has one of the functional groups protected by t-butyl group. The protection can be removed under acidic conditions. The propargyl group forms triazole linkages with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry. The PEG units help improving the water-solubility of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
This product is suitable for rapid extraction of RNA (include miRNA) from tissue, cells, blood, s and other clinical samples. RNA can be used directly for RT-PCR, quantitative RT-PCR, test of virus DNA and so on.
Details
Specifications
Features
Specifications
Main Functions
Isolation total RNA（miRNA）from tissue, cell using two columns and DNase plus reagent
Applications
RT-PCR, cDNA synthesis, second generation sequencing
Products
RNA, miRNA
Purification method
Mini spin column
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
Clinical tissues, cells, lymphocytes
Sample amount
Tissue: <20 mgCells: <5 x 106
Yield
2-50μg
Elution volume
≥30μl
Time per run
≤25 minutes
Principle
This product is based on silica column purification. Remove paraffin by Buffer DPS. Sample lysis withproteinase K digestion requires only 15 minutes. After lysis, samples are incubated at 80ºC for 15 minutes. Transfer to an adsorption column and RNA is adsorbed on the membrane, while protein is not adsorbed andis removed with filtration. After washing proteins and other impurities, RNA was finally eluted with low-saltbuffer.
Advantages
Efficient DNA removal – one step RNA extraction can effectively remove genomic DNA
High quality – one step RNA extraction reagent combined with silica gel column can obtain the highest concentration
Fast – the whole extraction only takes 15-25 minutes
Nontoxic – no toxic phenol chloroform extraction is required in the extraction
Kit Contents
Contents
IVD4121
Purification Times
50 Preps
HiPure DNA Mini Column Ⅱ
50
HiPure RNA Mini Columns
50
2ml Collection Tubes
150
Proteinase K
24 mg
Protease Dissolve Buffer
1.8 ml
DNase I
600 μl
DNase Buffer
6 ml
Buffer RTL
40 ml
RNA Digestion Buffer
15 ml
Buffer RWC*
20 ml
Buffer RW2*
20 ml
Nuclease Free Water
10 ml
Storage and Stability
Proteinase K should be stored at 2–8°C upon arrival. DNase I should be stored at -20°C. However, short-term storage (DNase I up to 1 weeks, Proteinase K up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions.
Document
This product is suitable for rapid extraction of RNA (include miRNA) from tissue, cells, blood, s and other clinical samples. RNA can be used directly for RT-PCR, quantitative RT-PCR, test of virus DNA and so on.
