GeneAb™ TIGIT

Description

Attogene’s Carbon Dioxide Enzymatic Assay Kit is a simple, direct method for measuring Carbon Dioxide levels in the environment. The assay uses a coupled enzyme assay to detect CO2 (as HCO3-) as follows. In the first step, the bicarbonate condenses with phosphoenol pyruvate to form oxalate (and phosphoric acid); this reaction is catalyzed by the enzyme Phosphoenolpyruvate Decarboxylase, PEPC. The oxalate is then enzymatically reduced by the enzyme Malate Dehydrogenase (using an NADH cofactor) to form malate and NAD+.

Description

Specifications

SRY (Sex Determining Region Y)-Box 10 (SOX-10), also known as transcription factor SOX-10, is a nuclear transcription factor that acts in regulation of embryonic development and in the specification and differentiation of cells of melanocytic lineage. SOX-10 is diffusely expressed in neurofibromas and schwannomas, and mutations in the SOX-10 gene are linked to Waardenburg-Shah and Waardenburg-Hirschsprung disease. Anti-SOX-10 has been shown to be sensitive for conventional, spindled, and desmoplastic melanoma, and has been used to detect metastatic melanoma and nodal capsular nevus in sentinel lymph nodes.

dsDNA Quantification HS Kit

The kit includes HS Dye, HS Dilution Buffer, and two DNA Standards.  The assay is accurate for DNA concentrations from 10 pg/µL to 100 ng/µL, and is highly selective for double-stranded DNA over RNA.

Features

• • • Optimized condition for using with the Qubit® Fluorometer
    • Uses the Qubit® dsDNA High Sensitivity assay setting
    • Linear range: 0.2-100 ng dsDNA
    • Cuts costs by 60%

DNA selectivity and sensitivity of the HS kit. A series of input DNA (20, 40, 60, 80, 100, and 120 ng) was used with or without the contamination of RNA.

Comparison of dsDNA HS kits. The performance of the BioDynami dsDNA HS kit is nearly identical to that of Thermo Fisher’s Qubit dsDNA HS kit.

Cat.# 40042 dsDNA Quantification HS Kit, 100 assays and 500 assays