DBCO-PEG5-TFP Ester is an amine-reactive, labeling reagent used to modify proteins, antibodies, and other amine-containing biopolymers. The hydrophilic polyethylene glycol (PEG) spacer arm provides a long and flexible connection that minimizes steric hindrance involved with ligation to complementary azide-containing molecules.

DBCO-PEG5-TFP Ester is an amine-reactive, labeling reagent used to modify proteins, antibodies, and other amine-containing biopolymers. The hydrophilic polyethylene glycol (PEG) spacer arm provides a long and flexible connection that minimizes steric hindrance involved with ligation to complementary azide-containing molecules.

Introduction

This product allows rapid and reliable isolation of high-quality genomic DNA from various soil, stool, and other environmental samples. Up to 500mg soil, 100mg stool, or 0.5g environmental samples can be processed in 60 minute. Purified DNA is suitable for PCR, restriction digestion, and next-generation sequencing.

Details

Specifications

Features	Specifications
Main Functions	Isolation total DNA from 250-500mg soil sample
Applications	PCR, southern blot and enzyme digestion, etc.
Purification technology	Magnetic beads technology
Process method	Manual or automatic
Sample type	Soil
Sample amount	250-500mg
Elution volume	≥50μl
Time per run	≤70 minutes

Principle

This product is based on the purification method of high binding magnetic particles. Soil sample is homogenized and then treated in a specially formulated buffer containing detergent to lyse bacteria, yeast, and fungal samples. Humic acid, proteins, polysaccharides, and other contaminants are removed using our propietary Absorber Solution. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing buffer to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.

Advantages

• High concentration – maximum extraction of total DNA from soil samples
• High purity – purified DNA can be directly used in various downstream applications

Kit Contents

Contents	D635601	D635602	D635603
Purification Times	48 Preps	96 Preps	5 x 96 Preps
MagPure Particles	2.5 ml	5 ml	22 ml
2ml Bead Tubes	48	96	400
Buffer SOL	60 ml	100 ml	500 ml
Buffer SDS	6 ml	10 ml	50 ml
Reagent DX	1 ml	1 ml	5 ml
Buffer PS	10 ml	20 ml	90 ml
Absorber Solution	10 ml	20 ml	90 ml
Buffer GDP	70 ml	150 ml	2 x 350 ml
Buffer GW1*	22 ml	44 ml	220 ml
Elution Buffer	20 ml	20 ml	60 ml

Storage and Stability

MagPure Particles and Absorber Solution should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

Experiment Data

This product allows rapid and reliable isolation of high-quality genomic DNA from various soil, stool, and other environmental samples. Up to 500mg soil, 100mg stool, or 0.5g environmental samples can be processed in 60 minute. Purified DNA is suitable for PCR, restriction digestion, and next-generation sequencing.