RNase activity in a convenient and sensitive fluorimetric assay that delivers results in real time. Great for Quality Testing for RNase contamination of materials and supplies.
RAA uses a novel RNA substrate tagged with a fluorescent reporter molecule (fluor) on one end and a quencher on the other. In the absence of RNases, the physical proximity of the quencher dampens fluorescence from the fluor to extremely low levels. When RNases are present, however, the RNA substrate is cleaved, and the fluor and quencher are spatially separated in solution. This causes the fluor to emit a bright green signal when excited by light of the appropriate wavelength. Fluorescence can be readily detected with a fluorometer. Since the fluorescence of the RAA Substrate increases over time when RNase activity is present, results monitored with a fluorometer can be evaluated kinetically. The sequence of the RAA Substrate has been carefully optimized to detect several RNases, including RNase A, RNase T1, RNase I, micrococcal nuclease, S1 nuclease, mung bean nuclease, and Benzonase.
Other Products
IVD5432 MagPure Circulating DNA Mini Kit
Product Info
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Product Info
Introduction
MagPure Circulating DNA Mini Kit is designed for purification of high quality circulating DNA (cfDNA) fromcell-free body fluids (such as plasma, serum). The purified DNA is suitable for direct use in downstream applications such as PCR, real-time PCR, biochip analysis and NGS.
Details
Specifications
Features
Specifications
Main Functions
Isolation circulating DNA from 0.2-0.6ml plasma, serum, body fluids
Applications
qPCR, NGS, etc.
Purification technology
Magnetic beads technology
Process method
Manual or automatic
Sample type
Serum, plasma
Sample amount
0.2-0.6ml
Elution volume
≥30μl
Time per run
≤50 minutes
Principle
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by elution buffer.
Advantages
Economy – less than 50% of the price of Qiagen and other imported products
Automatic – without labour
Kit Contents
Contents
IVD5432
Purification Times
200
MagPure Particles G
14 ml
Carrier RNA
310 μg
Proteinase K
180 mg
Protease Dissolve Buffer
10 ml
Buffer MLK
250 ml
Buffer MAW1
250 ml
Buffer MW2*
2 x 50 ml
Elution Buffer
60 ml
Contents
IVD5432-F-96A
IVD5432-F-96B
IVD5432-F-96C
Sample amount
200～350μl
400~700μl
Carrier RNA
110 μg
110 μg
Proteinase K
50 mg
100 mg
Protease Dissolve Buffer
5 ml
6 ml
Elution Buffer
15 ml
15 ml
Tip
1
1
Sample Plate A
500μl Buffer MLK
500μl Buffer MLK
Sample Plate B
/
500μl Buffer MLK
Wash Plate 1
700μl Buffer MAW1
700μl Buffer MAW1
Wash Plate 2
25μl Buffer MPG2700μl Buffer MW2
25μl Buffer MPG2700μl Buffer MW2
Wash Plate 3
700μl Buffer MW2
700μl Buffer MW2
Elution Plate
/
/
Contents
IVD5432-TL-06A
IVD5432-TL-06B
IVD5432-TL-06C
Sample amount
300~350μl
600~700μl
900~1050μL
Carrier RNA
310 μg
310 μg
310 μg
Proteinase K
50 mg
100 mg
150 mg
Protease Dissolve Buffer
6 ml
6 ml
10 ml
Elution Buffer
15 ml
15 ml
15 ml
DA-Tip
12
12
12
Row 1/7
600μl Buffer MLK
600μl Buffer MLK
600μl Buffer MLK
Row 2/8
/
600μl Buffer MLK
600μl Buffer MLK
Row 3/9
600μl Buffer MAW1
600μl Buffer MAW1
600μl Buffer MLK
Row 4/10
20μl Buffer MPG2600μl Buffer MW2
20μl Buffer MPG2600μl Buffer MW2
30μl Buffer MPG2900μl Buffer MAW1
Row 5/11
600μl Buffer MW2
600μl Buffer MW2
900μl Buffer MW2
Row 6/12
/
/
/
Storage and Stability
Proteinase K, Carrier RNA and MagPure Particles G should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance. Theremaining kit components can be stored dry at room temperature (15–25°C) and are stable for at least18 months under these conditions.The entire kit can be stored at 2–8°C, but in this case buffers should beredissolved before use. Make sure that all buffers are at room temperature when used.
Document
MagPure Circulating DNA Mini Kit is designed for purification of high quality circulating DNA (cfDNA) fromcell-free body fluids (such as plasma, serum). The purified DNA is suitable for direct use in downstream applications such as PCR, real-time PCR, biochip analysis and NGS.
Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:
> 2 years under recommended storage conditions
Analyte:
D-Gluconate, D-Glucono-δ-lactone
Assay Format:
Spectrophotometer, Microplate, Auto-analyser
Detection Method:
Absorbance
Wavelength (nm):
340
Signal Response:
Increase
Linear Range:
0.8 to 50 µg of D-gluconic acid per assay
Limit of Detection:
0.792 mg/L
Reaction Time (min):
~ 6 min
Application examples:
Wine, meat, processed meat (e.g. additives), fruit juice, dairy products, pharmaceuticals, paper and other materials (e.g. biological cultures, samples, etc.).
Method recognition:
Methods based on this principle have been accepted by ISO, DIN and GOST
The D-Gluconic Acid/D-Glucono-δ-lactone test kit is suitable for the specific measurement and analysis of D-gluconic acid/D-gluconolactone in foods and beverages.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).
Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.
All reagents stable for > 2 years after preparation
Very competitive price (cost per test)
Very rapid reaction
Mega-Calc™ software tool is available from our website for hassle-free raw data processing
Standard included
Suitable for manual, microplate and auto-analyser formats
Document
The D-Gluconic Acid/D-Glucono-δ-lactone test kit is suitable for the specific measurement and analysis of D-gluconic acid/D-gluconolactone in foods and beverages.
Methyltetrazine-PEG11-DBCO is a TCO reactive reagent with a DBCO group and water-soluble PEG spacer. This reagent can be used to convert azido-containing peptides or proteins into tetrazine-modified peptides or protein without catalyst or axillary reagents. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
Methyltetrazine-PEG11-DBCO is a TCO reactive reagent with a DBCO group and water-soluble PEG spacer. This reagent can be used to convert azido-containing peptides or proteins into tetrazine-modified peptides or protein without catalyst or axillary reagents. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
