Cell membrane preparations containing nicotinic AChRs that are ligand-gated ion channels that form pores in cells plasma membranes, mediating fast signal transmission at synapses. Nicotinic AChRs are involved in a wide range of physiological processes, and can be either neuronal or muscle-type. The membrane preparations we have developed are suitable for receptor binding assays in which muscle type nicotinic AChRs are needed. The membranes are tested in several functional binding assays and quality testing criteria to meet binding specifications.
Other Products
Bis-propargyl-PEG12
Product Info
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Product Info
Bis-propargyl-PEG12 has two alkyne groups at both ends of the linker. With the catalyzation of copper, the alkyne groups reacts with azide compounds to form stable triazole linkages. The PEG spacer enhances the water-solubility of the compound. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
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Bis-propargyl-PEG12 has two alkyne groups at both ends of the linker. With the catalyzation of copper, the alkyne groups reacts with azide compounds to form stable triazole linkages. The PEG spacer enhances the water-solubility of the compound. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
This product is suitable for rapid extraction of DNA from tissue, cells, blood, saliva, swabs, blood spots, semen and other clinical samples. DNA can be used directly for PCR, quantitative PCR, Southern Blot, test of virus DNA and so on.
Details
Specifications
Features
Specifications
Main Functions
Isolation total DNA from 1-100ul blood, FFPE, tissue and other samples
Applications
Second generation sequencing, PCR, real timePCR, etc.
Purification method
Monodisperse magnetic beads
Purification technology
Magnetic beads technology
Process method
Manual or automatic
Sample type
Anticoagulant blood, concentrated blood, buffy coat, lymphocytes and cultured cells
Sample amount
Body fluid : 10 – 200μl, Tissue : ≤10mg
Yield
10ng – 15μg
Elution volume
80 -100μl
Time per run
Principle
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.
Advantages
High binding force – suitable for handling DNA rich samples, such as whole blood, buffy coat, concentrated blood, etc.
High purity – carboxyl magnetic beads with weak surface adsorption, getting higher purity
General – can be used for various clinical samples
Kit Contents
Contents
IVD3101
Purification Times
200
MagBind Particles
4.5 ml
Proteinase K
90 mg
Protease Dissolve Buffer
10 ml
Buffer ATL
60 ml
Buffer AL
60 ml
Buffer BD*
20 ml
Buffer BXW1*
110 ml
Elution Buffer
30 ml
Storage and Stability
Proteinase K, MagBind Particles should be stored at 2–8°C upon arrival. However, short-termstorage (up to 18 weeks) at room temperature (15–25°C) does not affect their performance. The remainingkit components can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions.
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This product is suitable for rapid extraction of DNA from tissue, cells, blood, saliva, swabs, blood spots, semen and other clinical samples. DNA can be used directly for PCR, quantitative PCR, Southern Blot, test of virus DNA and so on.
For the enumeration of enterococci in water and other liquids by the membrane filtration technique.
Principle and Interpretation
The growth of the entire accompanying Gram-negative microbial flora is inhibited by sodium azide. Enterococci reduce 2,3,5-Triphenyl tetrazolium chloride (TTC) to give a red formazan inside the bacterial cell, their colonies are thus red. Nitrogen, minerals, and amino acids are provided by the tryptose whilst yeast extract supplies vitamins. Glucose acts as the carbon source, dipotassium phosphate buffers the medium, and agar-agar is the solidifying agent.
Formulation
Ingredients
/liter
Tryptose
20.0g
Yeast extract
5.0g
Glucose
2.0g
Disodium hydrogen phosphate
4.0g
Sodium azide
0.4g
2,3,5-Triphenyl Tetrazolium chloride
0.1g
Agar
10.0g
pH 7.2±0.1 at 25°C
Preparation
Dissolve 41.5 g in 1 L of purified water. Heat in boiling water and agitate frequently until completely dissolved. Sterilize by further heat for 20 minutes in the boiling water bath.
Quality Control
Cultural characteristics observed after incubation at 34-38°C for 40-48hours
Quality control strains
Growth
Colony color
Enterococcus faecalis ATCC29212
Good growth,PR≥0.5
deep red coloured colonies
Escherichia coli ATCC25922
Total inhibition
–
Sorage and Shelf Life
Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
Precautions
1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort
2. Keep container tightly closed after using to prevent clumping.
Waste Disposal
Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.
Document
Intended Use For the enumeration of enterococci in water and other liquids by the membrane filtration technique. Principle and Interpretation The growth of the entire accompanying Gram-neg……
