Bile Salt Export Pump (BSEP) is a member of the ATP-binding cassette (ABC) transporters, which mediates the transport of bile acid, taurocholate and other cholate conjugates across the hepatocyte canalicular membrane into the canaliculus. BSEP is associated with progressive familial intrahepatic cholestasis type 2 (PFIC2) and benign recurrent intrahepatic cholestasis type 2 (BRIC2). PFIC2 caused by mutations in the BSEP gene increases the risk of hepatocellular carcinoma in early life.
Specification
| 7-50x continuous zoom stereo microscope | XTL-205A | XTL-205B | XTL-204A | XTL-2048 | XTL-203A | XTL-203B | |
| Zoom Body | Binocular head,45°decline,360°rotate | ● | ● | ● | |||
| Trinocular head,45°decline,360*rotate | ● | ● | ● | ||||
| Eyepiece | WF10X22mm | ● | ● | ● | ● | ● | ● |
| Objective | zoom objective 0.7X—5X | ● | ● | ● | ● | ● | ● |
| Zoom Rate | 0.1:07.1 | ● | ● | ● | ● | ● | ● |
| Total Amplific- ation Rate | the standard configuration is 7X-50X | ● | ● | ● | ● | ● | ● |
| Auxiliary Lens | 0.5X,0.7X,1X,1.5X,2X For Option | O | O | O | O | O | O |
| WorkingDistance | 100mm | ● | ● | ● | ● | ● | ● |
| Diopter | 55mm-75mm | ● | ● | ● | ● | ● | ● |
| Focus arm | 76mm Diameter,32mm pole size | ● | ● | ● | ● | ● | ● |
| Plate | Black/White plate,Glass plate for option | ● | ● | ● | ● | ● | ● |
| Optional | Stand | Q | Q | ||||
| Stand | BI3 base.column sunnort unner and ower light source 3 WLED | ● | ● | ||||
| BL3-A base vertical arm bracket upper and lower light source 3 WLED | ● | ● | |||||
| B3 base column support has no light source | ● | ● | |||||
| External light source | LED illumination | Q | Q | Q | O | ||
| Optiona | Photography,camera accessories CCD Coupler | o | o | o | o | o | o |
| Optional | Display attachment Monitor | ||||||
Description
The DM1160 FluoroBand™ 50 bp Fluorescent DNA Ladder is a ready-to-use DNA ladder, which is pre-mixed with high sensitivity DNA binding fluorescent dye and loading dye for direct gel loading. The DNA Ladder DM1160 is composed of 17 individual DNA fragments: 1.5k, 1.2k, 1k, 900, 800, 700, 600, 500, 450, 400, 350, 300, 250, 200, 150, 100, and 50 bp derived from a mixture of PCR products and specifically digested plasmid DNA; these bands can be visualized when illuminated with 470 nm blue light or a UV light. This product contains two enhanced bands (500 bp and 200 bp) for easier reference. In addition, the low range Orange G tracking dye which mimics the migration of a 50 bp dsDNA during electrophoresis is also added for real time monitoring. Real time observation of the electrophoresis is also possible if compatible light source is fitted to the electrophoresis tank.
Features
Source
Phenol extracted PCR products and dsDNA digested with specific restriction enzymes, equilibrated in 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA.
Range
50 ~ 1,500 bp
Concentration
54 µg/ 500 µl
Recommended loading volume
5 µl/ well
Storage
Protected from light
Room temperature for 6 months
4°C for 12 months
-20°C for 24 months
The DM1160 FluoroBand™ 50 bp Fluorescent DNA Ladder is a ready-to-use DNA ladder, which is pre-mixed with high sensitivity DNA binding fluorescent dye and loading dye for direct gel loading. The DNA Ladder DM1160 is composed of 17 individual DNA fragments: 1.5k, 1.2k, 1k, 900, 800, 700, 600, 500, 450, 400, 350, 300, 250, 200, 150, 100, and 50 bp derived from a mixture of PCR products and specifically digested plasmid DNA; these bands can be visualized when illuminated with 470 nm blue light or a UV light. This product contains two enhanced bands (500 bp and 200 bp) for easier reference. In addition, the low range Orange G tracking dye which mimics the migration of a 50 bp dsDNA during electrophoresis is also added for real time monitoring. Real time observation of the electrophoresis is also possible if compatible light source is fitted to the electrophoresis tank.
Salmonella enterica have emerged as significant foodborne pathogens that pose a serious public health problem. The symptoms of salmonellosis may include diarrhea, fever, vomiting, and abdominal cramps with elderly, new-born, and immunocompromised individuals the most susceptible. S. enterica is a facultatively anaerobic Gram-negative bacterium that could survive low temperatures and freezing. The majority of the 1.3 billion annual cases of Salmonella-caused human gastroenteritis result from ingestion of contaminated food products, such as raw or undercooked meat, seafood, and eggs, as well as raw or unpasteurized milk and dairy products. Salmonella infections are also contracted following consumption of fresh fruits or vegetables that have been contaminated by infected fertilizer.
Salmonella enterica TaqMan PCR Kit, 24 reactions
Figure 1 / 3
Click for expanded view
Storage Conditions
The Salmonella enterica TaqMan PCR Kit Dx is shipped on dry ice. The components of the kit should be frozen upon arrival. If one or more of the components is not frozen when the kit is received, or if any of the components have been compromised during shipment, please contact Norgen Biotek for assistance. All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 3 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
| Component | Cat. TM32100 (24 rxns) |
|---|---|
| MDx TaqMan 2X PCR Master Mix Dx | 550 µL |
| S. enterica Primer & Probe Mix Dx | 2 x 70 µL |
| S. enterica Positive Control Dx – 200,000 copies/μL | 50 µL |
| Nuclease-Free Water | 2 x 1.25 mL |
| Product Insert | 1 |