Description
Specifications
| Clone | IHC002 |
| Source | Mouse Monoclonal |
| Positive Control | Breast Carcinoma |
| Dilution Range | 1:200 |
The Her2/Neu (c-erbB-2) proto-oncogene is a transmembrane receptor tyrosine kinase that is clinically indicated in a number of carcinomas. Overexpression of the c-erbB-2 protein has been associated with ductal breast cancer, as well as pulmonary and gastric adenocarcinomas. A correlation between Her2 and p53 has also been documented, as overexpression of both proteins has been associated with early invasion and metastasis in bladder cancer.
| Clone | IHC002 |
| Source | Mouse Monoclonal |
| Positive Control | Breast Carcinoma |
| Dilution Range | 1:200 |
The Norgen FullRanger 100 bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. Our FullRanger contains sixteen discrete fragments ranging from 100 bp to 5000 bp with higher intensity reference bands at 500 bp and 1000 bp. This Ladder is ideal for accurate visual determination in both large and small cloning applications.
Contents
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).
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FullRanger 100 bp DNA Ladder (Cat# 11800) – 100 loads
Ladder Properties:
• Sixteen discrete bands, ranging from 100 bp to 5000 bp
• Higher intensity reference bands at 500 bp and 1000 bp
| Fragment | Size (bp) | Mass (ng) |
| 1 | 5000 | 55 |
| 2 | 4000 | 44 |
| 3 | 3000 | 33 |
| 4 | 2500 | 28 |
| 5 | 2000 | 22 |
| 6 | 1500 | 17 |
| 7 | 1000 | 50 |
| 8 | 900 | 35 |
| 9 | 800 | 31 |
| 10 | 700 | 43 |
| 11 | 600 | 24 |
| 12 | 500 | 56 |
| 13 | 400 | 16 |
| 14 | 300 | 22 |
| 15 | 200 | 15 |
| 16 | 100 | 11 |
Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Storage:
Stable at room temperature. For longer term storage, -20°C is recommended.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.
Propargyl-PEG8-amine is a heterobifunctional reagent consisting of a propargyl group and an amine group. The amine group can form amide bonds with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde) etc. The propargyl group can form triazole linkage with azides in copper catalyzed Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Propargyl-PEG8-amine is a heterobifunctional reagent consisting of a propargyl group and an amine group. The amine group can form amide bonds with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde) etc. The propargyl group can form triazole linkage with azides in copper catalyzed Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
