Description
Specifications
| Clone | IHC409 |
| Source | Mouse Monoclonal |
| Positive Control | Colon, Colon Carcinoma |
| Dilution Range | 1:200 |
MutL Homolog 1 (MLH1) is a protein involved in the mismatch-repair pathway. This protein is commonly associated with hereditary non-polyposis colorectal cancer, as the MLH1 gene is frequently mutated in patients with this cancer. Studies have shown MLH1 to be deficient in a high percentage of patients with microsatellite instability, as well as endometrial and ovarian cancers. Use of Anti-MLH1 is optimized when paired in an IHC panel with MSH6, MSH2, and PMS2. Anti-MLH1 is useful in the detection of MLH1 in a number of normal and neoplastic tissues, and for identifying a loss of MLH1 in tumors that are microsatellite-unstable.
| Clone | IHC409 |
| Source | Mouse Monoclonal |
| Positive Control | Colon, Colon Carcinoma |
| Dilution Range | 1:200 |
K-RAFGA
SKU: 700004331
120 assays per kit
| Content: | 120 assays per kit |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | D-Galactose, Raffinose |
| Assay Format: | Spectrophotometer |
| Detection Method: | Absorbance |
| Wavelength (nm): | 340 |
| Signal Response: | Increase |
| Linear Range: | 4 to 83 µg of D-galactose per assay (i.e. approx. 12 to 250 µg of raffinose per assay) |
| Limit of Detection: | 21 mg/L |
| Reaction Time (min): | ~ 60 min |
| Application examples: | Cereal flours, soybean flour, by-products of sucrose manufacture and other materials. |
| Method recognition: | Used and accepted in food analysis |
The Raffinose/D-Galactose test kit allows for the specific and rapid measurement of raffinose and D-galactose in plant materials and food products.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).
View all of our monosaccharide test kit products.
Advantages
The Raffinose/D-Galactose test kit allows for the specific and rapid measurement of raffinose and D-galactose in plant materials and food products.
R-AMHR4
SKU: 700005032
200 assays per kit (4 vials)
| Content: | 200 assays per kit (4 vials) |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | α-Amylase |
| Assay Format: | Spectrophotometer, Auto-analyser |
| Detection Method: | Absorbance |
| Wavelength (nm): | 400 |
| Limit of Detection: | 0.05 U/mL |
| Reproducibility (%): | ~ 3% |
| Reaction Time (min): | ~ 30 min |
High purity α-Amylase HR Reagent – 4 vials for the measurement of α-amylase for research, biochemical enzyme assays and in vitro diagnostic analysis.
A new amylase assay reagent containing p-nitrophenyl α-D-maltoheptaoside (blocked) plus a thermostable α-glucosidase. The incorporation of this enzyme allows assays to be performed at temperatures up to 60oC and over the pH range 5.2-7.5.
See our complete list of assay reagents.
High purity α-Amylase HR Reagent – 4 vials for the measurement of α-amylase for research, biochemical enzyme assays and in vitro diagnostic analysis.
A new amylase assay reagent containing p-nitrophenyl α-D-maltoheptaoside (blocked) plus a thermostable α-glucosidase. The incorporation of this enzyme allows assays to be performed at temperatures up to 60oC and over the pH range 5.2-7.5.
See our complete list of assay reagents.
These kits provides a rapid method for the purification of total RNA from non-preserved saliva and nasal/throat swabs, and from preserved saliva collected on Norgen’s Saliva RNA Collection and Preservation Devices Dx (Cat. 53800) or preserved swabs collected in Norgen’s Total Nucleic Acid Preservative Tubes Dx (Cat. Dx69200). Purification is based on using Norgen’s proprietary resin separation matrix. RNA is preferentially purified from other cellular components such as proteins, without the use of phenol or chloroform. The chemistry employed in the kit allows the purification of total RNA, including viral and bacterial RNA, irrespective of size or GC content. The purified RNA is ideal for in vitro diagnostic use for medical purposes.
Saliva/Swab RNA Purification Kit Dx (Spin Column)
This kit is optimized to be used with any downstream application employing enzymatic amplification or other enzymatic modifications of RNA followed by signal detection or amplification. Any diagnostic results generated using the RNA isolated with Norgen’s Saliva / Swab RNA Purification Kit Dx in conjunction with an in vitro diagnostic assay should be interpreted with regard to other clinical or laboratory findings.
To minimize irregularities in diagnostic results, suitable controls for downstream applications should be used.
Norgen’s Saliva/Swab RNA Purification Kit Dx is intended for use by professional users such as technicians, physicians and biologists experienced and trained in molecular biological techniques.
Norgen’s Saliva/Swab RNA Purification Kit Dx does not provide a diagnostic result. It is the sole responsibility of the user to use and validate the kit in conjunction with a downstream in vitro diagnostic assay.
Saliva/Swab RNA Purification 96-Well Kit Dx (High Throughput)
This kit is optimized to be used with any downstream application employing enzymatic amplification or other enzymatic modifications of RNA followed by signal detection or amplification. Any diagnostic results generated using the RNA isolated with Norgen’s Saliva / Swab RNA Purification 96-Well Kit Dx in conjunction with an in vitro diagnostic assay should be interpreted with regard to other clinical or laboratory findings. To minimize irregularities in diagnostic results, suitable controls for downstream applications should be used. Norgen’s Saliva / Swab RNA Purification 96-Well Kit Dx is intended for use by professional users such as technicians, physicians and biologists experienced and trained in molecular biological techniques. Norgen’s Saliva / Swab RNA Purification 96-Well Kit Dx does not provide a diagnostic result. It is the sole responsibility of the user to use and validate the kit in conjunction with a downstream in vitro diagnostic assay.
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| Kit Specifications | |
| Sample Volume Range | 250 µL |
| Size of RNA purified | All sizes, including small RNA (<200 nt) |
| Minimum Elution Volume | 75 µL |
| Maximum Elution Volume | 100 µL |
| Time to complete 96 purifications | 30 minutes |
| Average Yield | ≥ 1 µg **varies from sample to sample |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. All solutions and plastics can be used until the expiration date specified on their labels.
| Component | Cat. Dx69100 (50 preps) | Cat. Dx69300 (192 preps) |
|---|---|---|
| Lysis Buffer A | 30 mL | 100 mL |
| Solution WN | 18 mL | 55 mL |
| Wash Solution A | 18 mL | 2 x 38 mL |
| Elution Solution A | 6 mL | 20 mL |
| Mini Spin Columns | 50 | – |
| 96-Well Isolation Plate (Deep Well) | – | 2 |
| Collection Tubes | 50 | – |
| 96-Well Collection Plate (Deep Well) | – | 2 |
| Elution Tubes (1.7 mL) | 50 | – |
| 96-Well Elution Plate (Deep Well) | – | 2 |
| Adhesive Tape | – | 4 |
| Product Insert | 1 | 1 |