Description
Specifications
| Clone | IHC659 |
| Source | Mouse Monoclonal |
| Positive Control | Seminoma, Dysgerminoma |
| Dilution Range | 1:200 |
Sal-Like Protein 4 (SALL4), is a zinc finger transcription factor found in germ cells and human blood progenitor cells, with functional involvement in modulating Oct-4 to maintain embryonic stem cell pluripotency. SALL4 is a useful marker for acute myeloid leukemia, B-cell acute lymphocytic leukemia, intratubular germ cell neoplasia, seminomas/dysgerminomas, and yolk sac tumors (both pediatric and postpubertal). Anti-SALL4 is used to detect embryonal carcinomas, hepatocellular carcinoma (HCC), gliomas, ovarian primitive germ-cell tumors, choriocarcinomas, spermatogonia, teratoma, gastric cancer, breast cancer, and lung cancer. Expression of SALL4 is often associated with poor prognosis in HCC, and with metastasis in endometrial cancer, colorectal carcinoma, and esophageal squamous cell carcinoma.
| Clone | IHC659 |
| Source | Mouse Monoclonal |
| Positive Control | Seminoma, Dysgerminoma |
| Dilution Range | 1:200 |
This product is suitable for extracting RNA from anticoagulant blood, lymphocytes, buffy coat, bone marrow, cultured cells and other samples.
Specifications
| Features | Specifications |
| Main Functions | Isolation total RNA from 1-1.5ml whole blood, 0.5-1ml bone marrow, buffy coat |
| Applications | RT-PCR, cDNA synthesis, second generation sequencing |
| Purification method | Polydisperse magnetic beads |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Sample type | anticoagulant blood, lymphocytes, buffy coat, bone marrow, cultured cells |
| Sample amount | 1-1.5ml whole blood, 0.5-1ml bone marrow |
| Yield | 1-30μg |
This product is suitable for extracting RNA from anticoagulant blood, lymphocytes, buffy coat, bone marrow, cultured cells and other samples. This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested by lysis buffer and protease, and RNA/DNA is released into the lysis buffer. Add binding solution and magnetic particles to adsorb RNA/DNA, while proteins are not adsorbed and removed. The particles adsorbed with DNA/RNA are washed with washing buffer to remove proteins and other impurities, then washed with ethanol to remove salt, and finally digested with DNase to remove DNA. RNA is recovered by adding binding solution, and finally the RNA is eluted with low salt buffer. The eluted RNA can be directly used for experiments such as RT-PCR,NGS and virus detection.
Advantages
Kit Contents
| Contents | R661101 | R661102 | R661103 |
| Purification Times | 48 Preps | 96 Preps | 480 preps |
| 10 x RBC Lysis Buffer | 50 ml | 2 x 50 ml | 4 x 100 ml |
| Proteinase K | 12 mg | 24 mg | 120 mg |
| Protease Dissolve Buffer | 1.8 ml | 1.8 ml | 10 ml |
| DNase I | 600 μl | 2 x 600 μl | 10 x 600 µl |
| DNase Buffer | 20 ml | 30 ml | 150 ml |
| MagPure Particles N | 1.2 ml | 2.5 ml | 11 ml |
| Buffer RTL | 30 ml | 60 ml | 300 ml |
| Buffer ALB2 | 40 ml | 60 ml | 300 ml |
| Buffer MW1* | 22 ml | 44 ml | 220 ml |
| Buffer MW2* | 20 ml | 50 ml | 2 x 100 ml |
| RNase Free Water | 10 ml | 20 ml | 60 ml |
Storage and Stability
DNase I should be shipped with ice pack or dry ice and stored at -20°C upon arrival. MagPure Particles N and Proteinase K should be stored at 2–8°C upon arrival. However, short-term storage(up to 8 weeks) at room temperature (15–25°C) does not affect their performance.The remaining kit components can be store at room temperature and are stable for up to 18 months under these conditions.
This product is suitable for extracting RNA from anticoagulant blood, lymphocytes, buffy coat, bone marrow, cultured cells and other samples.
Norgen’s EXTRAClean RNA Clean-Up and Concentration Micro-Elution Kit provides a rapid method for the purification, cleanup and concentration of up to 10 μg of RNA isolated using different methods including phenol/guanidine-based protocols, and from various upstream enzymatic reactions such as DNase treatment, labeling and in vitro transcription. The minimum recommended elution volume is 8 μL, which enables the concentration of small amounts of all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The RNA is preferentially purified from other reaction components such as proteins, RNases and nucleotides, without the use of phenol or chloroform. The EXTRAClean columns undergo stringent processing and rigorous quality control measures to minimize contamination traces, ensuring optimal results for sensitive applications such as NGS. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including end-point or quantitative reverse transcription PCR, Northern blotting, RNase protection and primer extension, expression array assays and next generation sequencing.
| Kit Specifications (Spin Column) | |
| Maximum Column Binding Capacity | 10 μg |
| Size of RNA Purified | All sizes, including miRNA and small RNA (< 200 nt) |
| Maximum Amount of Starting Material | 10 μg of RNA |
| Minimum Elution Volume | 8 μL |
| Time to Complete 10 Purifications | 20 minutes |
| Average Yields | ≥ 90% |
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