Description
1X MOPS-SDS Running Buffer Powder is used for electrophoresis on Q-PAGE™ Bis-Tris Precast Gel or polyacrylamide gels in Bis-Tris buffer system. It is convenient and universal for electrophoresis in Bis-Tris buffer system.
Features
Contents
50mM Tris Base, 50mM MOPS, 0.1% SDS, 1mM EDTA
Applications
Running buffer for sodium dodecyl sulfate-polyacryamide gel electrophoresis (SDS-PAGE) in Bis-Tris buffer system
Storage
Room temperature for 24 months
1X MOPS-SDS Running Buffer Powder is used for electrophoresis on Q-PAGE™ Bis-Tris Precast Gel or polyacrylamide gels in Bis-Tris buffer system. It is convenient and universal for electrophoresis in Bis-Tris buffer system.
This kit provides a fast, reliable and simple procedure for high throughput isolation of DNA from all types of soil samples including common soil samples and difficult soil samples with high humic acid content such as compost and manure. A combination of chemical and physical homogenization effectively lyses all microorganisms in the soil sample, and the kit removes all traces of humic acid using the provided Organic Substance Removal (OSR) Solution and Humic Acid Removal plate (HAR). Total genomic DNA can be isolated and purified from all the various microorganisms found in soil, such as bacteria, fungi and algae. The purified DNA is of the highest quality and is fully compatible with downstream PCR and qPCR applications and more for any metagenomic study, as all humic acid substances and PCR inhibitors are removed during the isolation.
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| Kit Specifications | |
| Binding Capacity Per Well | 50 μg |
| Maximum Loading Volume Per Well | 500 μL |
| Size of DNA Purified | All sizes |
| Maximum Amount of Starting Material | 250 mg |
| Time to Complete 96 Purifications | 50 minutes |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
| Component | Cat. 26560 (192 preps) |
|---|---|
| Lysis Buffer G | 2 x 100 mL |
| Lysis Additive A | 25 mL |
| Binding Buffer I | 25 mL |
| OSR Solution | 12 mL |
| Wash Solution A | 2 x 38 mL |
| Elution Buffer B | 30 mL |
| Bead Tubes | 200 |
| HAR Plate | 2 |
| 96-Well Filter Plate | 2 |
| Adhesive Tape | 4 |
| 96-Well Collection Plate | 4 |
| 96-Well Elution Plate | 2 |
| Product Insert | 1 |
