Introduction

HiPure Insect DNA Kits provides a simple and rapid solution for total DNA extraction of insect tissue samples. This kit is based on silica gel column purification technology without toxic phenol chloroform extraction and time-consuming alcohol precipitation. The whole extraction process only takes 30 minutes. HiPure Insect DNA Kit can process tissue samples less than 10mg at a time. Hipure Insect DNA 96 kit can process 96 insect tissue samples at a high throughput. The obtained DNA can be directly used in PCR, Southern blot, viral DNA detection and other experiments.

Details

Specifications

Features	Specifications
Main Functions	Isolation total DNA from <10 mg insect tissue
Applications	PCR, southern bolt and virus detection, etc
Purification method	Midi spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Insect tissue samples
Sample amount	<10 mg
Elution volume	≥15μl
Time per run	≤30 minutes
Liquid carrying volume per column	800μl
Binding yield of column	100μg

Principles

This product is based on silica column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).

Advantages

• High purity DNA – can be used in sensitive downstream applications such as multiplex and quantitative pcr
• Fast – several samples can be extracted in less than 30 minutes
• Good repeatability – suitable for extracting high-yield DNA from insect tissue samples
• Safe – without phenol chloroform extraction and alcohol precipitation

Kit Contents

Contents	D312902	D312903
Purification Times	50	250
HiPure DNA Mini Columns I	50	250
2ml Collection Tubes	50	250
Buffer ITL	30 ml	120 ml
Buffer IL*	30 ml	120 ml
Buffer GW1*	22 ml	110 ml
Buffer GW2*	20 ml	2 x 50 ml
Proteinase K	24 mg	120 mg
Protease Dissolve Buffer	1.8 ml	15 ml
Buffer AE	15 ml	60 ml

Storage and Stability

Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2–8°C, but in thiscase buffers should be redissolved before use. Make sure that all buffers areat room temperature when used.

HiPure Insect DNA Kits provides a simple and rapid solution for total DNA extraction of insect tissue samples. This kit is based on silica gel column purification technology without toxic phenol chloroform extraction and time-consuming alcohol precipitation. The whole extraction process only takes 30 minutes. HiPure Insect DNA Kit can process tissue samples less than 10mg at a time. Hipure Insect DNA 96 kit can process 96 insect tissue samples at a high throughput. The obtained DNA can be directly used in PCR, Southern blot, viral DNA detection and other experiments.

endo-BCN-PEG4-acid is a click chemistry reagent with a BCN group witha terminal carboxylic acid (CO2H). The terminal carboxylic acid is reactive with primary amine groups in the presence of activators (e.g. EDC, or HATU) to form a stable amide bond. The BCN group can react with azide -tagged compound or biomolecules. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

endo-BCN-PEG4-acid is a click chemistry reagent with a BCN group witha terminal carboxylic acid (CO2H). The terminal carboxylic acid is reactive with primary amine groups in the presence of activators (e.g. EDC, or HATU) to form a stable amide bond. The BCN group can react with azide -tagged compound or biomolecules. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

TD5B Large Capacity Low Speed Centrifuge

Features:

1. Brushless motor with simpler construction, more reliable performance, longer life and quietly running.
2. Automatically electric lid lock, super speed, over temperature protection and imbalance protection. The centrifuge body is made of high quality steel, safe and reliable.
3. Rotor is connected to spindle by specialized taper sleeve, loading simple and quick, no direction, safe and reliable.
4. Microprocessor control, TD5B with digital display which indicate the speed, time, RCF in operation, speed raising and reducing quick, operate simply.
5.10 kinds of accelerating and decelerating speed for your choice.

TD5B Technical Parameter：

Max. Speed	5000rpm
Max. RCF	4730×g
Max. Capacity	4×800ml
Time Range	0~99min
RPM/RCF Convert	Yes
Noise (dB)	≤ 58
Temperature	Normal
Acc/Dec	10 Kinds
Speed Accuracy	±20r/min
Temperature Accuracy	/
Voltage(V/Hz)	AC 220V/110V 50HZ/60HZ
Size (W x D x Hmm)	685×500×385mm
Net Weight(Kg)	67KG
Certificates	CE,ISO & Calibration report are available

Matched Rotors for TD5B

Order No	Rotor	Max Speed(r/min)	Volume(ml)	Max RCF(×g)
5B-1	Swing Rotor	4000	4×800ml	3450
5B-2	Swing Rotor	4000	4×750ml	3530
5B-3	Swing Rotor	4000	4×500ml	3380
5B-4	Swing Rotor	4000	4×4×96(well)	2940
5B-5	Microplate rotor	4000	2×4×96(well)	3210
5B-6			2×3×48(well)	2300
5B-7	Swing rotor	5000	4×1×50ml	4730
			4×1×100ml	4730
		4000	4×2×100ml	3020
		4000	4×2×50ml	3200
		4000	4×4×15ml	3200
		4000	4×6×15ml	3200
5B-8	Swing rotor	4000	4×8×15ml	3200
		4000	4×12×5ml vacuum tube	2480
		4000	4×12×7ml vacuum tube	2760
		4000	4×12×10ml vacuum tube	2880
		4000	4×8×10/7/5ml vacuum tube	2790
		4000	4×6×10/7/5ml vacuum tube	2790
		4000	4×4×10/7/5ml vacuum tube	2790
5B-9	Swing rotor	4000	4×24×5ml vacuum tube	2480
		4000	4×31×5ml Khan tubes	2480
5B-10	Swing rotor	4000	4×24×7ml vacuum tube	3140
		4000	4×31×7ml Khan tubes	3140
		4000	4×30×7ml vacuum tube	3140
		4000	4×30×5ml vacuum tube	3140
		4000	4×40×7ml vacuum tube	3270
		4000	4×18×10ml vacuum tube	3140
5B-11	Swing rotor	4000	Dairy bottle	3830
5B-12	Fixed rotor	5000	6×15ml	2540
5B-13		5000	12×15ml	3080
5B-14		5000	24×15ml	3500
5B-15		5000	30×15ml	3830
5B-16		5000	4×50ml	2520
5B-17		5000	6×50ml	2850
5B-18		5000	12×50ml	3860
5B-19		4000	24×50ml	2970
5B-20		5000	4×100ml	2630
5B-21		5000	6×100ml	3130
5B-22		4000	12×100ml	2970

Adapter for 800ml and 500ml bucket

VolumeCup	5ml	7ml	10ml	15ml	50ml
800ml（Round Cup）	23pcs/cup	23	19	14	5
500ml（Round Cup）	18pcs/cup	18	14	9	3
500ml（Square Cup）	20pcs/cup	20	16	12	5

TD5B large capacity centrifuge max capacity can be reached to 4x800ml, can with different adapters for small tubes, 50ml, 15ml, 10ml, 7ml, 5ml, 2ml and so on.