Introduction
029150 Phenylanine Deaminase Reagent
Usage:For Phenylalanine Decarboxylase Test.
Specification: 10ml.
10ml
029150 Phenylanine Deaminase Reagent
Usage:For Phenylalanine Decarboxylase Test.
Specification: 10ml.
Clone | IHC697 |
Source | Mouse Monoclonal |
Positive Control | Colon Cancer |
Dilution Range | 1:200 |
Thymidylate Synthase (TS) is a crucial enzyme responsible for the synthesis of 2′-deoxythymidine-5′-monophosphate (dTMP) a precursor for thymidylate which is necessary for DNA replication and repair from 2′-deoxyuridine-5′-monophosphate (dUMP). In terms of cancer, TS is an important target for cancer treatment as the inhibition of TS and therefore nucleotide synthesis necessary for cell growth has shown to be a vital part for successful treatment against colorectal, pancreatic and breast cancers.
Mycoplasma is a common and serious contaminant of cell cultures. Mycoplasma is often not visible and does not respond to antibiotics, and therefore it is a major issue that requires monitoring and early detection. Up to 30% of cell cultures may be contaminated with Mycoplasmas, the main contaminants being the species M. orale, M. laidlawii, M. arginini and M. hyorhinis. These organisms produce a variety of effects on the infected cells in culture, including changes in metabolism growth, viability and morphology, thereby altering the phenotypic characteristics of the host cells. Therefore there is an absolute requirement for routine, periodic assays to diagnose possible Mycoplasma contamination of all cell cultures, particularly continuous or established cell lines.
Mycoplasma TaqMan PCR Kit, 100 reactions
Mycoplasma TaqMan PCR Probe/Primer Set and Controls, 100 reactions
For research use only and NOT intended for in vitro diagnostics.
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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
Component | Cat. TM33150 (100 preps) | Cat. TM33110 (100 preps) |
---|---|---|
MDx TaqMan 2X PCR Master Mix | 2 x 700 μL | – |
Mycoplasma Primer & Probe Mix | 280 μL | 280 μL |
Mycoplasma Positive Control | 150 μL | 150 μL |
Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
Product Insert | 1 | 1 |
K-GLOX
SKU: 700004296
200 assays (manual) / 2000 assays (microplate) / 1960 assays (auto-analyser)
Content: | 200 assays (manual) / 2000 assays (microplate) / 1960 assays (auto-analyser) |
Shipping Temperature: | Ambient |
Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
Stability: | > 2 years under recommended storage conditions |
Analyte: | Glucose Oxidase |
Assay Format: | Spectrophotometer, Microplate, Auto-analyser |
Detection Method: | Absorbance |
Wavelength (nm): | 510 |
Signal Response: | Increase |
Linear Range: | 0.01 to 0.08 U/mL of glucose oxidase per assay |
Limit of Detection: | 10 U/L |
Reaction Time (min): | ~ 20 min |
Application examples: | Enzyme preparations, and other materials (e.g. biological cultures, samples, etc.). |
Method recognition: | Novel method |
The Glucose Oxidase assay kit is a simple procedure for the rapid and reliable measurement and analysis of glucose oxidase activity in industrial enzyme preparations and bread improver mixtures.
View more of our assay kits for enzyme activities.
Advantages
The Glucose Oxidase assay kit is a simple procedure for the rapid and reliable measurement and analysis of glucose oxidase activity in industrial enzyme preparations and bread improver mixtures.