Usages: For isolating lactose-fermenting Gram-negative enteric bacilli.
Principle: Peptones are sources of nitrogen and other nutrients. Lactose is a fermentable carbohydrate. When lactose is fermented, alocal pH drop around the colony causes a color change in the pH indicator (neutral red) and bile precipitation. Bile salts,bile salts no. 3, oxgall and crystal violet are selective agents that inhibit growth of gram-positive organisms. Agar is the solidifying agent.
Formulation（per liter）:
Pancreatic Digest of Gelatin
17.0 g
Peptones (meat and casein)
3.0 g
Lactose Monohydrate
10.0 g
Sodium Chloride
5.0 g
Bile Salts
1.5 g
Agar
13.5 g
Neutral Red
30.0 mg
Crystal Violet
1 mg
Final pH
7.1±0.2
How to use： 1.Suspend 50 g in 1 L of distilled or deionized water. Heat to boiling to dissolve completely. Autoclave at 121°C for 15 minutes.
2.Transfer 1 mL of Soybean–Casein Digest Broth to 100 mL of MacConkey Broth, and incubate at 42 to 44 for 24 to 48 hours. Subculture on a plate of MacConkey Agar at 30 to 35 deg.C for 18 to 72 hours.
Quality control：
Item
The name and number of strain
PR/G
Reaction
Growth rate
E.Coli ATCC8739
PR≥0.7
Rose-red
Characteristic difference
Proteus mirabilis CMCC(b)49005
PR≥0.7
Colorless, no swarming
Selective
Staphylococcus aureus ATCC6538
G≤1
－
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of three years.
Specifications: 250g/bottle
Other Products
Saliva DNA Collection and Preservation Devices (50)
Product Info
Document
Product Info
Overview
Reliable and cost-effective
Non-invasive, user-friendly sample collection and preservation in one convenient kit
Preserved DNA is stable for 2 years at ambient temperature
High quality DNA is suitable for sensitive downstream applications including PCR, qPCR, sequencing, SNP analysis, microarrays, RFLP and Southern Blot Analysis
Norgen’s Saliva DNA Collection and Preservation Devices are designed for 1) simple and non-invasive saliva collection and 2) preservation of DNA in saliva samples at ambient temperature. Each of the 50 Saliva DNA Collection and Preservation Devices consists of 3 components:
Saliva Collection Funnel and Collection Tube
Collection Tube Cap
Norgen’s Saliva DNA Preservative contained within a sealed squeezable ampoule
Saliva samples are collected by spitting inside the Collection Funnel which has been assembled with the Collection Tube. After collecting the required volume of saliva the Collection Funnel is removed and the contents of the Preservative Ampoule are then added and mixed with the collected saliva. The Saliva Collection Tube is subsequently sent to the laboratory for DNA isolation and analysis. DNA can be isolated from the preserved saliva samples using Norgen’s Saliva DNA Isolation Kit (Cat. RU45400). Each of Norgen’s Collection Tubes is labeled with a unique serial number that can be used for secure and anonymous tracking of the sample. The saliva DNA in preserved samples is stable for more than 2 years at room temperature. This kit is ideal for collecting and preserving DNA samples for epidemiological and population studies.
Saliva DNA Preservative
Norgen’s Saliva DNA Preservative is an aqueous storage buffer designed for rapid cellular lysis and subsequent preservation of DNA from fresh specimens. The buffer prevents the growth of Gram-negative and Gram-positive bacteria and fungi, and also inactivates viruses allowing the resulting non-infectious samples to be handled and shipped safely. In addition, the buffer eliminates the need to immediately process or freeze samples and allows the samples to be shipped to centralized testing facilities at ambient temperature. The components of the buffer allow samples to be stored for more than 2 years without any detectable DNA degradation.
When stored at room temperature, unused Norgen Saliva Preservative Tubes are stable through to the collect-before date without any reduction in performance. Please see the device insert or tube label for collect-before date.
Once collected, saliva is stable for more than 2 years when kept tightly sealed and stored at room temperature.
The Collection Tube, the Collection Funnel and the Device Container of each Individual Saliva DNA Collection and Preservation Device are recyclable.
Kit Components
Cat. RU49000 (50 Devices)
Individual Saliva DNA Collection and Preservation Devices
50
Donor Procedure Flowchart
1
Product Insert
1
Individual Saliva DNA Collection and Preservation Device Contents
The Opentrons Thermocycler Module GEN2 is a fully automated on-deck thermocycler, providing hands-free PCR in a 96-well plate format. Compatible with Opentrons hardware and software to allow for fully automated PCR reaction setup and thermocycling on your Opentrons robot. The heated lid and disposable seal fit tightly over the plate, ensuring efficient sample heating and minimal evaporation.
If you choose to include the Opentrons Flex Caddy and Calibration Adapter, your order will ship in 20 business days.
Document
The Opentrons Thermocycler Module GEN2 is a fully automated on-deck thermocycler, providing hands-free PCR in a 96-well plate format. Compatible with Opentrons hardware and software to allow for fully automated PCR reaction setup and thermocycling on your Opentrons robot. The heated lid and disposable seal fit tightly over the plate, ensuring efficient sample heating and minimal evaporation.
If you choose to include the Opentrons Flex Caddy and Calibration Adapter, your order will ship in 20 business days.
Fast and easy processing using rapid and convenient spin-columns
Isolate high quality and high yield DNA
DNA is free of inhibitors and ready for downstream use including SNP (single nucleotide polymorphism) and short-tandem repeat (STR) genotyping
This kit provides a rapid method for the isolation and purification of DNA from formalin-fixed paraffin-embedded (FFPE) tissue samples. Using formalin to fix tissues leads to crosslinking of the nucleic acids and proteins, and the process of embedding the tissue samples can also lead to fragmentation of the nucleic acids over time. Norgen’s FFPE DNA Purification Kit provides conditions that allow for the partial reversing of the formalin modifications, resulting in a high quality and yield of nucleic acids. The purified DNA is of high yield and integrity and is free of inhibitors, ready for use in a number of downstream applications including qPCR, mutation screening, microarray analysis, sequencing, single nucleotide polymorphism (SNP) and short-tandem repeat (STR) genotyping. The protocol can be completed in as little as 1 hour.
Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. The Proteinase K should be stored at -20°C upon arrival and after reconstitution. This kit is stable for 1 year after the date of shipment.
