For isolation and cultivation of Listeria monocytogenes.
Principle:
Peptone provide carbon and nitrogen sources; yeast extract powder and starch provide carbon and nitrogen sources, vitamins and growth factors; sodium chloride maintains osmotic equilibrium; glucose carbon source; agar as medium coagulant.
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
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20mins High Sensitivity Isothermal DNA Amplification Kit For Accurate Detection
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Product Description
20mins High Sensitivity Isothermal DNA Amplification Kit For Accurate Detection
Product Detail
Applicable equipment:
It is recommended to use the Isothermal fluorescence detector developed by Amp-future, which is also suitable for fluorescence quantitative PCR apparatus with market known brands.
Kit Storage and term of Validity
Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.
Term of Validity: 14 months
Isothermal nucleic acid Principle Summary
This kit is based on a rapid nucleic acid amplification technology at room temperature and constant temperature: at room temperature and constant temperature (generally 39℃~42℃), with the help of auxiliary proteins and single-strand binding proteins,the recombinase and primers form a complex;Source search and combine the target homology domain, at this time,a D-loop region is formed at the homology position and strand exchange begins;along with the dissociation of the recombinase from the complex,the polymerase also binds to the 3′ end of the primer and begins chain extension. At the same time, relying on the function of exonuclease, adding specific molecular probes designed according to the template, and using fluorescence monitoring equipment can realize real-time monitoring of the amplification process of the target fragment.
Isothermal nucleic acid Product Features
1/ High sensitivity and specificity, short reaction time.
2/ The reagent form is freeze-dried, stable and easy to operate.
Technical Parameters:
Parameters
Details
Product Name
DNA Isothermal Amplification Kit EXO
Manufacturer
Amp-future
Storage Temperature
-20°C
Kit Components
Enzymes, Buffers ,Reagents
Packaging
48 Tests/box
Detection Limit
500-1000copies/µL
Shipping
ICE
Test Time
5-20mins
Isothermal nucleic acid Applications
Suitable for DNA isothermal rapid amplification kit(fluorescent type)
Primer: Require pair of nucleotide primers with the length of 25-35 bp.
Fluorescent Probe:Require the suitable length is 46-52nt.
DNA fluorescent kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.
Notes
1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.
2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.
It is suitable for the extraction and purification of viral nucleic acid and free nucleic acid. It can bind the nucleic acids in solution through hydrophobic, hydrogen bonding and electrostatic interaction under high salt conditions, without binding with other impurities (such as proteins), and quickly separate nucleic acids from biological samples. The operation is safe and simple, which is very conducive to the automatic and high-throughput extraction of short fragments or low abundance nucleic acids.
Product characteristics
Super paramagnetism and high magnetic response, saving operation time and increasing sample recovery efficiency.
Good suspension performance, dispersion and resuspension, which is conducive to efficient nucleic acid binding and recovery.
Good physical and chemical stability to ensure repeatability.
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Note: Price not include shipment & duty, contact us to get full quote.
Magnetic beads are specially designed for nucleic acid extraction and purification, with good suspension performance. The surface is modified with a large number of carboxyl groups, which can bind the nucleic acid in the sample through hydrophobic, hydrogen bonding and electrostatic interaction under high salt and low pH conditions, without binding with other impurities (such as proteins), and quickly separate nucleic acid from biological samples. The operation is safe and simple. It is beneficial to the automation and high throughput extraction of nucleic acid.
Mastitis is the single most costly disease of dairy cattle resulting in the reduction of milk yield and quality. The inflammation of the utter is mainly caused by infection of various bacteria. Streptococcus dysgalactiae, a Lancefield group C α-hemolytic gram-positive bacteria, is a common pathogen in subclinical and clinical mastitis causing substantial economic losses in dairy herds. Streptococcus dysgalactiae has been isolated from infected mammary glands and teat injuries and is transmitted primarily during milking. However, detection of S. dysgalactiae in other cow tissues such as tonsils, mouth and vagina, and occurrence of S. dysgalactiae mastitis during the non-lactating period in herds, suggests that the organism also behaves as an environmental pathogen.
Storage Conditions and Product Stability All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival.
