Beef extract powder and acid hydrolyzed casein protein provides nitrogen, vitamins and amino acids; soluble starch absorption of toxic metabolites; agar as medium coagulant.
Formulation(per liter):
Agar 17g
Beef extract 2g
Soluble starch 1.5g
Acid hydrolysis of casein 17.5g
Final pH7.3 ± 0.2
How to use:
1.Suspend 38g in 1 L of distilled water , stirring heated to boiling until completely dissolved, dispensing flask, 121 autoclave for 15min.
2.Diluted and treated samples.
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
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Bis-propargyl-PEG3
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Bis-propargyl-PEG3 is a crosslinker with alkyne groups at both ends of the molecule. The alkyne groups react with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry to form a stable triazole linkage. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
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Bis-propargyl-PEG3 is a crosslinker with alkyne groups at both ends of the molecule. The alkyne groups react with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry to form a stable triazole linkage. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Urine Exfoliated Cell and Bacteria RNA Purification Kit
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Product Info
Overview
Purify all sizes of RNA (including microRNA) without the need for phenol
Isolate and detect total RNA from 1 mL and up to 50 mL urine
Provides high-quality RNA for sensitive applications – isolate RNA from as little as 100 cells
Rapid processing time from sampling to downstream testing
Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
This kit provides a rapid spin column method for the isolation and purification of total RNA (including microRNA) from exfoliated cells that have been shed into the urine from the urinary tract. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The RNA is preferentially purified from other cellular components such as proteins, as well as from the contaminating species found in urine such as glucose and salts, without the use of phenol or chloroform. The purified RNA is of the highest integrity and can be used in a number of downstream applications including real-time RT-PCR, qRT-PCR, Northern blotting, NGS, and expression array assays. Multiple samples can be processed in 20 minutes.
Background
RNA biomarkers from exfoliated cells can be used as non-invasive tools for a number of diagnostic and research applications including the diagnosis and monitoring of bladder, kidney, or other urinary-tract cancers.
~1μg RNA per 1 x 105 cells (For Exfoliated Cells – Varies due to cell density sample)~0.5μg RNA per 1 x 107 cells(For Bacteria – Varies due to cell density sample)
Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
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Document
Propargyl-PEG4-methylamine is a PEG linker that can be react with azide compounds in copper catalyzed azide-alkyne Click Chemistry reactions. The methylamine group is reactive with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde) etc. The PEG spacer increases hydrophilicity of the linker in aqueous environment. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
