027340 PYG Broth Base

Introduction

Usages:
For cultivation of bifidobacteria and lactic acid metabolites test.

Principle:
Peptone, yeast extract provide nitrogen, vitamins, and growth factors; glucose as
fermentable sugars; cysteine – HCl, vitamin K1, hemin, magnesium sulfate, calcium
chloride, sodium chloride, sodium bicarbonate training offers various bifidobacteria growth factor; dipotassium hydrogenphosphate, potassium dihydrogenphosphate buffer.

Formulation ( per liter):
Peptone 20g
Glucose 5g
Yeast powder 10g
Cysteine – hydrochloride 0.5g
Calcium chloride 0.008g
Magnesium sulfate 0.008g
Dipotassium hydrogen phosphate 0.04g
Potassium dihydrogen phosphate 0.04g
Sodium bicarbonate 0.4g
Sodium chloride 0.08g
Final pH 6.0 ± 0.2

Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.

Contents	D635901D	D635902D	D635903D
Purification Times	48	96	480
MagPure Particles N	1.1 ml	2.2 ml	11 ml
Buffer ATL	40 ml	80 ml	2 x 200 ml
Buffer BGL	40 ml	80 ml	2 x 200 ml
Buffer BST1	60 ml	120 ml	550 ml
Buffer GW2*	20 ml	25 ml	2 x 50 ml
Proteinase K	48 mg	110 mg	2 x 220 mg
Protease Dissolve Buffer	5 ml	10 ml	30 ml
DTT	235 mg	235 mg	3 x 235 mg
Elution Buffer	10 ml	10 ml	30 ml

Product	Contents and volume	D6359D-TL-06
Buffer BGL		80 ml
Buffer ATL		80 ml
Proteinase K		110 mg
Protease Dissolve Buffer		10 ml
DTT Powder		2 x 235 mg
Elution Buffer		5 ml
AS-Tip		12 pcs
2.0ml V bottom plate	Row 1/7：empty	6 plates
	Row 2/8：500μl Buffer BST1
	Row 3/9：500μl Buffer BST1
	Row 4/10：20μl MagPure Particles N 500μl Buffer GW2 Row 5/11：500μl Buffer GW2 (ethanol ~350ul)
	Row 6/12：60μl Elution Buffer

Product	Contents and volume	D6359D-F-96	D6359D-F-48
Buffer BGL		80 ml	40 ml
Buffer ATL		80 ml	40 ml
Proteinase K		110 mg	48 mg
Protease Dissolve Buffer		10 ml	5 ml
DTT Powder		235 mg	235 mg
Elution Buffer		5 ml	5 ml
96-Tip (AS)		1	1
Sample Plate 1	500μl Buffer BST1	1	1
Sample Plate 2	500μl Buffer BST1	1	1
Wash Plate 1	20μl MagPure Particles N500μl Buffer GW2	1	1
Wash Plate 2	500μl Buffer GW2	1	1
Elute Plate	60μl Elution Buffer	1	1

Content:	58 assays (manual) / 580 assays (microplate) / 700 assays (auto-analyser)
Shipping Temperature:	Ambient
Storage Temperature:	Short term stability: 2-8^oC, Long term stability: See individual component labels
Stability:	> 2 years under recommended storage conditions

Analyte:	D-Sorbitol, D-Xylitol
Assay Format:	Spectrophotometer, Microplate, Auto-analyser
Detection Method:	Absorbance
Wavelength (nm):	492
Signal Response:	Increase
Linear Range:	1.0 to 20 µg of D-sorbitol (or xylitol) per assay
Limit of Detection:	0.20 mg/L
Reaction Time (min):	~ 15 min
Application examples:	Diabetic foods (e.g. honey, jam and chocolate), dietetic foods, chewing gum, candies, fruit juice (e.g. apple juice), ice-cream, sweets, bakery products (e.g. desserts), marzipan, paper (and cardboard), cosmetics, pharmaceuticals and other materials (e.g. biological cultures, samples, etc.).
Method recognition:	Methods based on this principle have been accepted by IFU and AIJN

Detail

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