027340 PYG Broth Base

Overview

• Extract total RNA (including microRNA) from FFPE samples
• No phenol extraction step
• Includes DNase for optional on-column DNA removal
• Isolated RNA is of the highest quality and integrity
• Isolate a diversity of RNA species
• Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen’s NGS services
• Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

Norgen’s FFPE RNA Purification Kits provide a rapid method for the isolation and purification of total RNA (including microRNA) from formalin-fixed paraffin-embedded (FFPE) tissue samples in as little as 1 hour. Using formalin to fix tissues leads to crosslinking of the RNA and proteins, and the process of embedding the tissue samples can also lead to fragmentation of the RNA over time. Norgen’s FFPE RNA Purification Kits provide conditions that allow for the partial reversing of the formalin modifications, resulting in a high quality and yield of RNA. These kits are able to purify all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA), depending on the age of the FFPE tissue as fragmentation of the RNA is known to occur over time. The RNA is preferentially purified from other cellular components without the use of phenol or chloroform.

FFPE RNA Purification Kit (Spin Column)

Maximum loading volume of 650 μL per column, and a maximum binding capacity of 50 μg per column.

FFPE RNA Purification 96-Well Kit (High Throughput)

Purification is based on 96-well column chromatography using Norgen’s proprietary resin as the separation matrix. Purification can be performed using either a vacuum manifold or centrifugation. Maximum loading volume of 400 μL per well, and a maximum binding capacity of 50 μg per well.

Details

Supporting Data

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Kit Specifications
Maximum Binding Capacity Per Well	50 μg
Maximum Loading Volume Per Well	400 μL
Size of RNA Purified	All sizes, including small RNA (< 200 nt)
Maximum Amount of Starting Material:	5 slices of < 20 µm thick paraffin  25 mg of unsectioned block

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The DNAse I should be stored at -20°C upon arrival. The Proteinase K should be stored at -20°C upon arrival and after reconstitution. This kit is stable for 1 year after the date of shipment.

Component	Cat. 25300 (50 preps)	Cat. 25400 (2 x 96 preps)
Digestion Buffer A	25 mL	2 x 25 mL
Buffer RL	30 mL	2 x 30 mL
Enzyme Incubation Buffer	6 mL	2 x 6 mL
Wash Solution A	38 mL	2 x 38 mL
Elution Solution A	6 mL	2 x 20 mL
Proteinase K	12 mg	2 x 20 mg
DNase I	1 vial	2 x 500μL
Micro Spin Columns	50	–
96-Well Incubation Plate	–	2
96-Well Plate	–	2
Adhesive Tape	–	8
Collection Tubes	50	–
96-Well Collection Plate	–	2
Elution Tubes (1.7 mL)	50	–
96-Well Elution Plate	–	2
Product Insert	1	1

DBCO-PEG12-amine is a PEG linker which contains DBCO and amine moieties. The DBCO group is commonly used in copper-free Click Chemistry reactions. The amine group is reactive with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde), etc. The hydrophilic PEG spacer increases the water solubility of the compound. Reagent grade, for research purpose.

DBCO-PEG12-amine is a PEG linker which contains DBCO and amine moieties. The DBCO group is commonly used in copper-free Click Chemistry reactions. The amine group is reactive with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde), etc. The hydrophilic PEG spacer increases the water solubility of the compound. Reagent grade, for research purpose.

LCD touch screen floor standing large capacity refrigerated centrifuge

Features

1. Microprocessor control, High resolution LCD display with touch screen functions. Parameters (speed, RCF, time)in running can be edited and changed.

2. Program memory up to 100 kinds. It can store all the parameters automatically in operation, and can use directly when start up next time.

3. Hold function

4. 10 Acceleration & 10 deceleration soft brake system.

5. Brush-less frequency motor with simpler construction, more reliable performance, longer life and quietly running.

6.  Automatic lid lock, automatic door opening and closing with automatically rotor lid opening.(Optional)

7. super speed, over temperature protection and imbalance protection. The centrifuge body is made of high quality steel, safe and reliable.

8.  Adapters are available by experiment requirements.                          

9. 3 tiers protection steel cover, safe and reliable.

10. CE EMC IEC 61010 certificate.

GL12 Technical Parameter：

Max. Speed	12000rpm
Max. RCF	22650×g
Max. Capacity	4×1000ml
Timer	1~9h59min
RPM/RCF Convert	Yes
Noise (dB)	≤ 60
Temperature Range	-20~40℃
Acc/Dec	10 Kinds
Speed Accuracy	±20r/min
Temperature Accuracy	±1℃
Voltage(V/Hz)	AC 220V/110V  50HZ/60HZ
Size (L x W x Hmm)	780×675×830mm
Net Weight(Kg)	260KG
Certificates	CE,ISO & Calibration report are available

Matched Rotors for GL12

Order No.	Rotor No.	Max Speed (rpm)	Max Volume(ml)	Max.RCF（×g）
L12-1	Angle Rotor	12000	6×250/300ml	22650
L12-2	Angle Rotor	8000	6×500ml	11740
L12-3	Swing Rotor	4000	4×1000ml	4060
L12-4	Microplate Rotor	4000	4×4×96well	2940
L12-5		4000	2×4×96well	2490
L12-6	Angle Rotor	10000	4×500ml	16110

Model:
GL12

Brand:
Yingtai

Code:
8421199090

Download:
Brochure of GL12 centrifuge touch screen.pdf