Introduction
Usages: For the total microbial count in urine.
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
500g
Usages: For the total microbial count in urine.
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
K-GCROLGK
SKU: 700004293
70 assays (manual) / 700 assays (microplate) / 600 assays (auto-analyser)
| Content: | 70 assays (manual) / 700 assays (microplate) / 600 assays (auto-analyser) |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | Glycerol |
| Assay Format: | Spectrophotometer, Microplate, Auto-analyser |
| Detection Method: | Absorbance |
| Wavelength (nm): | 340 |
| Signal Response: | Increase |
| Linear Range: | 1.0 to 35 µg of glycerol per assay |
| Limit of Detection: | 0.37 mg/L |
| Reaction Time (min): | ~ 7 min |
| Application examples: | Wine (and grape juice), beer, spirits, vinegar, marzipan, fruit juices, soft drinks, toothpaste, honey, tobacco, paper (and cardboard), cosmetics, pharmaceuticals, soap and other materials (e.g. biological cultures, samples, etc.). |
| Method recognition: | Novel method |
The Glycerol GK test kit is a simple, reliable and accurate method for the measurement and analysis of glycerol in beverages, foodstuffs and other material. Based on use of ADP-glucokinase and increase in absorbance on conversion of NAD+ to NADH.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).
Explore our complete range of alcohol assay kit products.
Advantages
The Glycerol GK test kit is a simple, reliable and accurate method for the measurement and analysis of glycerol in beverages, foodstuffs and other material. Based on use of ADP-glucokinase and increase in absorbance on conversion of NAD+ to NADH.
The Primerdesign genesig Kit for Shigella species (Shigella_spp) genomes is designed for the in vitro quantification of Shigella_spp genomes. The kit is designed to have a broad detection profile. Specifically, the primers represent 100% homology with over 95% of the NCBI database reference sequences available at the time of design.
The dynamics of genetic variation means that new sequence information may become available after the initial design. Primerdesign periodically reviews the detection profiles of our kits and when required releases new versions.
The target sequence is within the virulence plasmid pCP301 (VirA) which is carried by nearly all clinical isolates of shigella. This target has previously been shown to be a good genetic marker for Shigella in other real time PCR based studies (Hiroshi Fukushima et.al 2003.) The primers and probe sequences in this kit have 100% homology with over 95% of reference sequences in the NCBI database based on a comprehensive bioinformatics analysis.
Exceptional value for money
Rapid detection of all clinically relevant subtypes
Positive copy number standard curve for quantification
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target
Accurate controls to confirm findings
MagPure Circulating DNA Rich Kit designed for purification of high quality circulating DNA (cfDNA) from cell-free body fluids (such as plasma, serum). The purified DNA is suitable for direct use in downstream applications such as PCR, real-time PCR, biochip analysis and NGS.
Specifications
| Features | Specifications |
| Main Functions | Rich small fragment cfDNA from 0.6ml serum plasma, remove DNA fragments＞500bp |
| Applications | qPCR, NGS, etc. |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Sample type | Serum, plasma |
| Sample amount | 0.6ml |
| Elution volume | |
| Time per run |
This product is based on the purification method of high binding magnetic particles. The sample is lysedand digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, Large DNA(>500bp) will be adsorbed on the surface of magBindparticles. After removal of large size, small size of DNA(<500bp) will be adsorbed on the surface ofMagPure Particle F and impurities such as proteins will be removed without adsorption. The adsorbedparticles were washed with washing solution to remove proteins and impurities, washed with ethanol toremove salts, and finally DNA
| Contents | 1292750 | 12927200 |
| Purification Times | 50 preps | 200 preps |
| MagPure Particles F | 1.2 ml | 4.5 ml |
| MagBind Particles | 1.2 ml | 4.5 ml |
| Proteinase K | 24 mg | 90 mg |
| Protease Dissolve Buffer | 1.8 ml | 10 ml |
| Buffer MLB | 30 ml | 120 ml |
| Buffer GDP | 15 ml | 60 ml |
| Buffer MAW1 | 40 ml | 250 ml |
| Buffer MW2* | 20 ml | 50 ml |
| Elution Buffer | 15 ml | 60 ml |
Storage and Stability
Proteinase K, MagBind Particles and MagPure Particles F should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.
MagPure Circulating DNA Rich Kit designed for purification of high quality circulating DNA (cfDNA) from cell-free body fluids (such as plasma, serum). The purified DNA is suitable for direct use in downstream applications such as PCR, real-time PCR, biochip analysis and NGS.