For the rapid detection and of coliforms and E. coli.
Principle:
Peptone and yeast extract powder provides carbon and nitrogen sources and trace elements; sodium chloride maintains osmotic equilibrium; agar as medium coagulant; dodecyl sulfate inhibit Gram-positive bacteria; chromogenic substrate were mixed occurrence of coliforms and E. coli enzyme corresponding specific reactions, hydrolysis of the substrate, the release of the color groups, in a pale yellow plates coliforms appears orange-red colonies while E.coli appears blue-green colonies.
Formulation (per liter):
Peptone 15.0g
Yeast extract powder 3.0g
Sodium chloride: 5.0g
Sodium lauryl sulfate: 0.1g
Agar: 12.0g
Mixed chromogenic substrate: 6.77g
Final pH 7.0 ± 0.2
How to use: 1. Weigh 41.9g of the product, adding , 1.0 L of distilled or deionized water, heated to boiling stir until completely dissolved, dispensing into flask, 115 autoclaved 10minutes.
2, Take 25.0g or 25.0mL of sample with sterile procedures, added to the flask containing 225.0mL of sterile phosphate buffered saline (or saline) ,shaken thoroughly homogenized with a homogenizer or a 1:10 dilution of 1min solution, diluted 1:10 and then continue to select the appropriate serial dilutions of three, the two plates inoculated with each dilution, poured dissolved by heating and cooled to about 45 medium.
3, observe the results.
Quality control:
This product appears light yellow after pouring on plate, these strains were inoculated after 36 ± 1 18 ~ 24h culture growth in the following table.
Bacteria name bacteria NO. growth situation feature
Escherichia coli ATCC25922 good blue-green colonies
Citrobacter ATCC8090 good orange-red colonies
Salmonella typhimurium CMCC50115 good colorless colonies
Enterococcus faecalis ATCC29212 suppressed —–
Storage: Store in a dark, cool and dry place, tighten the caps immediately after use. Storage period of two years.
Blood samples contain rich DNA, including mitochondrial DNA, genomic DNA, circulating DNA (mostly released into blood after tumor cell apoptosis) in white blood cells, as well as parasitic viral or microbial DNA. These DNA are important parameters in clinical testing or diagnosis, which are also valuable materials for medical research. There are three main issues with extracting DNA from blood samples:
1. The sample is highly infectious, posing great harm to operators and the environment.
2. The source of DNA is complex and aportion of the nucleic acid, such as viral DNA or free DNA, may be lost during the operation, leading to downstream detection failure;
3. Blood sample contains a large amount of impurities and inhibitory factors.
Currently there are many methods available for extracting DNA from whole blood samples, such as phenol chloroform extraction, salting out method, etc. However, these methods require pre-treatment of blood sample, which removes red blood cells and isolate white blood cells in the first step. Due to the requirement that it cannot inactivate or kill pathogens during the process of removing red blood cells, the waste liquid (red blood cell lysate) and consumables may be contaminated by pathogens and become infectious, posing a danger to the entire laboratory environment and operators. In addition, during the process of removing red blood cells, useful nucleic acid information such as viruses, microorganisms, or circulating DNA is also lost, leading to experiment or detection failures.
The HiPure Blood DNA Kits series provided by Magen Company uses silica gel column purification technology, which can directly lyse whole blood samples without the need for white blood cell separation. Whole blood samples are directly mixed with lysates and proteases, resulting in the inactivation of pathogens, greatly reducing the infectivity, environmental pollution, and the chance of operators being infected. Due to the direct lysis and digestion of samples, except lymphocyte DNA, other circulating DNA as well as DNA from viruses and microorganisms, can also be recovered.
This product provides fast and easy methods for purification of total DNA for reliable PCR and Southern blotting. Total DNA (e.g., genomic, viral, mitochondrial) can be purified from tissue, whole blood, plasma, serum, buffy coat, bone marrow, other body fluids, lymphocytes, cultured cells.
Details
Specifications
Features
Specifications
Main Functions
Isolation total DNA from blood, tissue, culture cells, swab, blood spots using 96 plate
Applications
PCR, southern bolt and virus detection, etc
Purification method
96 well plate
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
Blood, serum, plasma, milk, saliva, and other liquid samples and cultured cells
Sample amount
Elution volume
Time per run
Liquid carrying volume per column
Binding yield of column
Principle
This product is based on silica column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
Advantages
High quality DNA – meet a variety of downstream applications, including PCR, qPCR, enzyme digestion, hybridization, etc.
High throughput – 96 samples can be processed simultaneously
Kit Contents
Contents
D311701
D311702
Purification Times
1 x 96
4 x 96
HiPure gDNA Plate
1
4
96 well Plate (2.2ml)
1
4
1.6ml Collection Plate
1
4
0.5ml Collection Plate
1
4
Silicon Seal Tape
1
4
Seal Film
5
25
Buffer ATL
30 ml
100 ml
Buffer AL
30 ml
100 ml
Buffer DW1
60 ml
250 ml
Buffer GW2
50 ml
2 x 100 ml
Proteinase K
50 ml
200 ml
Protease Dissolve Buffer
5 ml
15 ml
Buffer AE
30 ml
120 ml
Storage and Stability
Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
Blood samples contain rich DNA, including mitochondrial DNA, genomic DNA, circulating DNA (mostly released into blood after tumor cell apoptosis) in white blood cells, as well as parasitic viral or microbial DNA. These DNA are important parameters in clinical testing or diagnosis, which are also valuable materials for medical research. There are three main issues with extracting DNA from blood samples:
This product supplies a simple and rapid extraction of total RNA from plant and fungal samples. The kit is based on super paramagnetic particles purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction process takes only 60 minutes. Purified RNA is ready for downstream applications such as RT-PCR, virus RNA testing and so on. MagPure RNA Kits buffers can be used for both manual extraction process and automatic nucleic acid extraction machines. This Kits is suitable for extracting RNA from ≤5×106 cultured cells, 20mg tissue and <50mg plant samples.
Details
Specifications
Features
Specifications
Main Functions
Isolation total RNA from 50mg plant using magnetic particles
Applications
RT-PCR, cDNA synthesis, second generation sequencing
Purification method
Polydisperse magnetic beads
Purification technology
Magnetic beads technology
Process method
Manual or automatic
Sample type
Plant and fungus samples
Sample amount
≤50mg
Yield
2-100μg
Time per run
≤60 minutes
Principle
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. After adding magnetic particles and binding solution, RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally RNA was eluted by Elution Buffer.
Advantages
High quality – high purity total RNA can be directly used in various sensitive downstream applications
Safe – no phenol chloroform extraction required
Fast – several samples can be extracted in 60 minutes by column method
Universal – two lysates suitable for most plant or fungal tissue samples
Kit Contents
Contents
R664101
R664102
R664103
Purification Times
48 Preps
96 Preps
480 Preps
MagPure RNA Particles
1.7 ml
3.5 ml
18 ml
DNase I
600 μl
2 x 600 μl
10 x 600 μl
DNase Buffer
30 ml
40 ml
200 ml
Buffer PRC1
40 ml
70 ml
350 ml
Buffer RL
40 ml
70 ml
350 ml
Buffer MCB*
18 ml
30 ml
150 ml
Buffer MW1*
22 ml
44 ml
220 ml
Buffer MW2*
20 ml
50 ml
2 x 100 ml
RNase Free Water
10 ml
15 ml
120 ml
Storage and Stability
MagPure RNA Particles should be stored at 2–8°C upon arrival. DNase I should be stored at -20°C. However, short-term storage (DNase I up to 1 weeks, MagPure RNA Particles up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under theseconditions.
Document
This product supplies a simple and rapid extraction of total RNA from plant and fungal samples. The kit is based on super paramagnetic particles purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction process takes only 60 minutes. Purified RNA is ready for downstream applications such as RT-PCR, virus RNA testing and so on. MagPure RNA Kits buffers can be used for both manual extraction process and automatic nucleic acid extraction machines. This Kits is suitable for extracting RNA from ≤5×106 cultured cells, 20mg tissue and
