Description

Attogene’s Mercury Lateral Flow Kit can be used for the screening of Mercury in water and food (fish) samples at 10 ppb in a laboratory setting.  Unlike the field-based kit, the lab kit is intended for a more technical end user who will be evaluating samples in a laboratory setting.  The instructions include method for extraction from food samples.

Mercury contamination is a serious worldwide environmental problem. As it is difficult to detoxify by chemical or biological methods, gradual Mercury ion accumulation in the nervous and cardiovascular systems of the human body can subsequently cause serious diseases.   Long-term health consequences of drinking Mercury-contaminated food and water include brain, heart, kidney, lungs  and immune system problems for adults, and the physical and mental development delays in infants and children.  Attogene’s Mercury Lateral Flow test gives results conforming of 10ppb or greater.  Using the supplied pipette, simply fill the vial with your water sample, place the water into the sample port and wait 5 minutes.

Applications for Fish and Shellfish possible:

Mercury Levels in Commercial Fish and Shellfish (1990-2012)

Fish with recognized high concentrations of Mercury:

• Tuna, Grouper, Mackerel, Weakfish, Bluefish, Sablefish, Halibut, Bass Chilean, Shark, Marlin, Tilefish,

Screening of Mercury in water samples at 10 ppb
Format: 10 tests (5 tests/5 controls)
Run Time: 5 Minutes

Introduction

This product is suitable for rapid extraction of DNA from tissue, cells, blood, saliva, swabs, blood spots, semen and other clinical samples. DNA can be used directly for PCR, quantitative PCR, Southern Blot, test of virus DNA and so on.

Details

Specifications

Principle

This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.

Advantages

• High binding force – suitable for handling DNA rich samples, such as whole blood, buffy coat, concentrated blood, etc.
• High purity – carboxyl magnetic beads with weak surface adsorption, getting higher purity
• General – can be used for various clinical samples

Kit Contents

Storage and Stability

Proteinase K, MagBind Particles should be stored at 2–8°C upon arrival. However, short-termstorage (up to 18 weeks) at room temperature (15–25°C) does not affect their performance. The remainingkit components can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions.

This product is suitable for rapid extraction of DNA from tissue, cells, blood, saliva, swabs, blood spots, semen and other clinical samples. DNA can be used directly for PCR, quantitative PCR, Southern Blot, test of virus DNA and so on.

Introduction

Usages:

For antimicrobial susceptibility testing.

Principle:

Beef extract powder and acid hydrolyzed casein protein provides nitrogen, vitamins and amino acids; soluble starch absorption of toxic metabolites; agar as medium coagulant.

Formulation(per liter):

Agar 17g

Beef extract 2g

Soluble starch 1.5g

Acid hydrolysis of casein 17.5g

Final pH7.3 ± 0.2

How to use:

1.Suspend 38g in 1 L of distilled water , stirring heated to boiling until completely dissolved, dispensing flask, 121 autoclave for 15min.

2.Diluted and treated samples.

Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.

500g