Usages: For differentiating enteric bacteria based on urease activity by adding 40% sterile urea solution.
Principle: Peptone provides the carbon and nitrogen; maintain a balanced osmotic sodium chloride; potassium dihydrogen phosphate is buffers; decomposing bacteria urease urea medium, produce large amounts of ammonia, agar as medium coagulant.
Formulation (per liter): Peptone 1g Sodium chloride 5g Glucose 1g Ppotassium dihydrogen phosphate 2g Phenol red 0.012g Agar 12g Final pH7.2 ± 0.2
How to use: 1.Suspend 21g in 1L of distilled or deionized water. Heat with frequent agitation and boil to completely dissolve the powder. Distribute into flasks. Autoclave at 121 for 15 minutes. cooling to 50-55 and adding 40% urea solution. 2.Diluted and treated samples.
Storage: Store in a dark, cool and dry place, tighten the cap immediately after use. Storage period of three years.
Other Products
AZtaq™ DNA Polymerase
Product Info
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Product Info
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AZtaq™ DNA Polymerase is a high-quality DNA polymerase, originating form Thermus aquaticus. Being highly thermostable, AZtaq is ideal for use in polymerase chain reaction (PCR) applications.
The enzyme catalyses the synthesis of a complementary DNA strand using a primed DNA or cDNA strand as template. It possesses 5’-3’ exonuclease activity while lacking 3’-5’ proofreading activity.
AZtaq is compatible with the use of dUTP, enabling highly efficient removal of carry-over contamination with Cod UNG.
Key Features
Excellent qPCR Performance
Compatible with dUTP
Thermostable
Applications
PCR/qPCR
Figures
Properties
Quality Control
ArcticZymes is dedicated to the quality of our products. AZtaq is manufactured at our ISO 13485 certified facility in Norway.
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AZtaq™ DNA Polymerase is a high-quality DNA polymerase, originating form Thermus aquaticus. Being highly thermostable, AZtaq is ideal for use in polymerase chain reaction (PCR) applications.
The Kit is specially designed for simultaneous purification of genomic DNA and total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue sections. Purified DNA/RNA is suitable for use in applications such as real-time PCR and Pyrosequencing.
Details
Specifications
Features
Specifications
Main FunctionsC
Co-isolation total RNA and DNA from FFPE tissue
Applications
RT-PCR, cDNA synthesis, PCR and second-generation sequencing, etc.
Purification method
Polydisperse magnetic beads
Purification technology
Magnetic beads technology
Process method
Manual or automatic
Adaptive instrument
Nucleic acid extractor and pipetting workstation
Sample type
FFPE slice, FFPE puncture sample, embedded tissue
Sample amount
No more than six 10µm sections of 150 mm2 surface area or three 20µm sections of 150 mm2 surface area.
Principle
FFPE samples are incubated in an optimized lysis buffer, which results in the release of RNA and precipitation of DNA. After centrifugation, the RNA-containing supernatant and DNA-containing pellet are then processed separately to purify RNA and DNA. After adding magnetic particles and binding solution, DNA/RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA was eluted by RNase Free Water.
Advantages
Post digestion sorting – higher DNA and RNA yields
Economy – the price is much lower than imported reagents
Kit Contents
Contents
IVD3026
Purification Times
200 Preps
MagBind Particles
9.0 ml
Proteinase K
180 mg
Protease Dissolve Buffer
10 ml
Buffer DPS
150 ml
Buffer FRL
40 ml
Buffer ATL
40 ml
Buffer AL
80 ml
Buffer BXW1*
110 ml
RNase Free Water
30 ml
Storage and Stability
Proteinase K and MagBind Particles should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
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The Kit is specially designed for simultaneous purification of genomic DNA and total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue sections. Purified DNA/RNA is suitable for use in applications such as real-time PCR and Pyrosequencing.
Features of the resDNASEQ CHO Residual DNA Quantitation kit include:
Simpler and Rapid
Only three steps will be need for Sample Preparation and All components of the Sample Preparation Kit can be stored at room temperature.
Only one Reagent for qPCR;
Only 1.5 hours will be needed for the whole test.
Accurate
Perfect amplification curve, good amplification efficiency and good precision.
Highly sensitive quantitation using proven TaqMan™ real-time qPCR technology.
Limit of Detection (LOD): 0.01 fg/μL; Limit of Quantification (LOQ): 0.3 fg/μL.
The recovery rate of different concentration samples in the linear range is between 70% and 130%.
Kit Performance
Fig 1. Only three steps will be need for Sample Preparation and only 20 minitutes will be taken for Sample Preparation.
Fig 2. Seven concentration samples of 0.3fg/μL, 3fg/μL, 30fg/μL, 300fg/μL, 3pg/μL, 30pg/μL, 300pg/μL were detected. CV of each concentration was < 30%, Regression coefficient associated with standard solutions was 0.99992, and amplification efficiency was 100.370%.
Fig 3. Five concentration samples of 0.1 fg/μL, 0.3 fg/μL, 0.5 fg/μL, 1 fg/μL and 3 fg/μL were detected, and 10 multiple wells were detected for each concentration. The CV of concentrati on values of samples with 0.3 fg/μL and above concentrations were less than 30%.
Fig 4. DNA recovery can be determined by including samples spiked with known DNA amounts which are prepared from the corresponding DNA standards. Typically, the range for this value varies from 70% to 130%.
Fig 5. Only one Reagent for qPCR MIX.
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Note: Price not include shipment & duty, contact us to get full quote. The resDNASEQ CHO Residual DNA Quantitation kit is designed for the quantification of residual DNA from CHO, in cell lines which are used for production of biopharmaceutical products. The Ducky Bio residual DNA CHO Assay, based on proven real-time qPCR technology, makes testing of residual DNA from the Chinese hamster ovary (CHO) cell line rapid, specifc. The PCR-based assay is sensitive and specific for DNA from the CHO cell line and not subject to detection of human or environmental DNA that might be introduced during sample handling. The kit was developed to meet the sensitivity requirements defined by WHO (10 ng CHO DNA per therapeutic dose).
