
K-AMYLSD
SKU: 700004263
160 assays (manual) / 640 assays (auto-analyser)
K-AMYLSD
SKU: 700004263
160 assays (manual) / 640 assays (auto-analyser)
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit. Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
Primer and probe mix (150 reactions)
Reverse Transcription, target specific primers (RNA genome viruses only)
Copy number standard curve (sufficient for multiple standard curves)
Internal extraction control – Read through VIC channel*
Endogenous control (150 tests)
RNAse/DNAse free water
*alternative fluorophores available on request
Spring Viremia of Carp is caused by Rhabdovirus carpio, a bullet-shaped RNA virus. The disease has been reported in common carp (or koi) (Cyprinus carpio), grass carp (Ctenopharyngodon idella), bighead carp (Aristichthys nobilis), silver carp (Hypophthalmichthys molitrix), and Crucian carp (Carassius carassius), a close relative of the goldfish. Recent evidence suggests that common goldfish (C. auratus) are also susceptible.
The disease was initially diagnosed in Yugoslavia (Fijan et al. 1971). Since then, it has been identified in other European countries, Russia, and the Middle East. Mortality has reached 70% in yearling carp from European populations. Adult fish can also be affected but to a lesser degree.
Exceptional value for money
Rapid detection of all clinically relevant subtypes
Positive copy number standard curve for quantification
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target
Accurate controls to confirm findings
This kit enables the rapid purification of amplified DNA products from PCR mixes. It is able to effectively remove PCR by-products including primers, dimers, enzymes, unincorporated nucleotides and mineral oil from the desired PCR product. The purified PCR products are fully compatible with restriction enzyme digestion, ligation into vectors, labeling, sequencing and more. This kit can also be used as an alternative to organic extraction and ethanol precipitation to clean up various enzymatic reactions.
The kit is also available in a 96-well format for high-throughput PCR purification. Purification with the 96-well plate can be performed using either a vacuum manifold or centrifugation.
Figure 1 / 2
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Kit Specifications – Spin Column | |
Column Binding Capacity | 10 μg |
Size of DNA Purified | 100 – 15,000 bp |
Average Recovery | > 90% |
Average Primer Removal | > 90% |
Minimum Elution Volume | 30 μL |
Time to Complete 10 Purifications | 15 minutes |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
Component | Cat. 14400 (50 preps) | Cat. 45700 (250 preps) | Cat. 24800 (192 preps) |
---|---|---|---|
Binding Buffer C | 30 mL | 5 x 30 mL | 3 x 30 mL |
Wash Solution A | 12 mL | 2 x 20 mL | 2 x 38 mL |
Elution Buffer B | 8 mL | 2 x 30 mL | 2 x 15 mL |
Spin Columns | 50 | 250 | – |
Collection Tubes | 50 | 250 | – |
96-Well Plate | – | – | 2 |
Adhesive Tape | – | – | 4 |
96-Well Collection Plate | – | – | 2 |
Elution Tubes (1.7 mL) | 50 | 250 | – |
96-Well Elution Plate | – | – | 2 |
Product Insert | 1 | 1 | 2 |
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Tel : 081-875-1869 , 02-328-7179
Email : hej@a3p-scientific.com
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