β-Amylase Assay Kit (Betamyl-3)

Everything you need to run a trial PACE Genotyping Reaction on your existing lab equipment. Each PACE Trial Kit includes Test DNA samples, PACE Genotyping Assays, PACE Master Mix and a comprehensive PACE Genotyping Trial Kit Manual.

About

Everything you need to run a trial PACE® allele-specific PCR Genotyping Reaction on your existing lab equipment. Each PACE Trial Kit includes Test DNA samples, PACE Genotyping Assays, PACE Master Mix and a comprehensive PACE Genotyping Trial Kit Manual.

WHO IS THIS TRIAL KIT FOR?

Anyone who wants to try PACE genotyping reagents in their lab for the first time with a set of validated DNA samples, SNP assays and PACE Master Mix.

TRIAL KIT OVERVIEW

Step 1. Dispense each of the three trial DNA samples (DNA 1, 2 and 3) plus water (No Template Control) in triplicate onto a PCR plate using the suggested volumes.

Step 2. Combine appropriate volumes of PACE Genotyping Master Mix with PACE Genotyping Assay in a tube, as directed, then mix.

Step 3. Dispense the combined mixtures into each of the wells containing DNA using volumes indicated. Each test now contains a complete PACE Genotyping Reaction.

Step 4. Seal your PCR plate with an optically clear seal and centrifuge to ensure all components are at the bottom of the wells.

Step 5.Thermally cycle the reaction plate using the thermal cycling conditions provided.

Step 6. Read the plate and compare data produced with the expected results provided in the manual. Simple!

PACE MECHANISM

More information on the PACE genotyping chemistry and how it works can be found here: www.3crbio.com/#pace. PACE allele-specific PCR is used for the detection of SNPs, Indels and other sequence variants.

REQUIRED COMPONENTS

• qPCR machine or Thermocycler + Fluorescent plate reader
• PCR plate or equivalent and appropriate optically clear seal
• PCR-grade water

qPCR machine or Thermocycler + Fluorescent plate reader

PCR plate or equivalent and appropriate optically clear seal

PCR-grade water

Description

Fentanyl (FYL) Detection Kit (Rapid Lab) is a lateral flow chromatographic immunoassay for the qualitative detection of Fentanyl/Norfentanyl in liquid and powder substances at the cut-off concentration of 200 ng/mL.

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It is specific for Fentanyl screening with no significant cross reactivity to other opiates, such as Morphine and Heroin.

Interpretation of test results are: positive (one line), negative (two lines), invalid (no lines or no control line).

• • Sample: Liquid / Powder
• • Format: Cassette
• • Sensitivity: 200 ng/mL
• • Specificity: Fentanyl
• • Accuracy: > 98%
• • Time-to-Result: 10 minutes
• • Storage Condition: 2-30°C/36-86°F
• • Test Principle: Lateral Flow Immunoassay

For professional institutions use only, not for self-testing.

Learn More:

• Fentanyl Facts  from CDC
• Fentanyl Test Strips: A Harm Reduction Strategy

Rapid screening test for the qualitative detection of Fentanyl (FYL)
Format: 10 tests
Run Time: 10 Minutes

Biotin-PEG4-alkyne is a PEG linker containing a biotin group and a terminal alkyne. Biotin is useful for affinity-based applications such as pull-down assays or for ligating with streptavidin proteins while terminal alkynes are used in copper (I) click chemistry with azides to form stable triazoles with a target molecule. The inclusion of a PEG linker in this molecule improves its aqueous solubility.

Biotin-PEG4-alkyne is a PEG linker containing a biotin group and a terminal alkyne. Biotin is useful for affinity-based applications such as pull-down assays or for ligating with streptavidin proteins while terminal alkynes are used in copper (I) click chemistry with azides to form stable triazoles with a target molecule. The inclusion of a PEG linker in this molecule improves its aqueous solubility.