Principle: Peptone provide nitrogen, vitamins and growth factors; sodium chloride to maintain osmotic equilibrium; lactose into fermentable sugars.
Formulation（per liter）:
Pancreatic Digest of Gelatin
20 g
Lactose Monohydrate
10g
Dehydrated Ox Bile
5g
Bromocresol Purple
10mg
final pH
7.3±0.2
How to use： 1.Suspend 35g in 1 L of distilled water , stirring heated to boiling until completely dissolved, dispensing flask, 121 ℃ autoclave for 15min. 2.Diluted and treated samples.
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
Specifications: 250g/bottle
Other Products
ProteoSpin™ Urine Protein Concentration Kits
Product Info
Document
Product Info
Overview
Simultaneous concentration, desalting and buffer exchange of total urinary proteins
No molecular weight cutoff allows for isolation of all sizes of proteins and peptides
Purification is based on spin column chromatography that uses Norgen’s resin separation matrix
These kits concentrate urine proteins while simultaneously removing salts, urea, and other urine contaminants. There is no molecular weight cut-off and therefore the columns capture total urinary proteins and peptides of all sizes making them ideal for biomarker discovery work, differential expression of proteins in various diseases, or other diagnostic research. The columns are convenient, rapid and easy to use and thus offer significant time savings over classic dialysis protocols. The resulting high-quality protein sample is concentrated and free from the original sample salts, thus preparing the sample conveniently for downstream proteomic applications including SDS-PAGE, 2D Gels, MALDI-TOF, LC/MS, LC/MS/MS, whole protein mass spectrometry, western blotting, protein microarrays, and more.
ProteoSpin™ Urine Protein Concentration Micro Kit
Each spin column is able to concentrate and desalt up to 200 μg of urine proteins. Twelve samples can be processed in 20 minutes.
ProteoSpin™ Urine Protein Concentration Midi Kit
The ProteoSpin™ Urine Protein Concentration Midi Kit provides a fast and simple procedure for concentrating dilute solutions of urine proteins from 1 to 5 mL inputs of urine. Each mini spin column is able to concentrate and desalt up to 3 mg of urine proteins in 30 minutes.
ProteoSpin™ Urine Protein Concentration Maxi Kit
The ProteoSpin™ Urine Protein Concentration Maxi Kit provides a fast and simple procedure for concentrating dilute solutions of urine proteins from 2 to 20 mL inputs of urine. Each maxi spin column is able to concentrate and desalt up to 4 mg of urine proteins in 45 minutes.
*Yield will depend on the health status of the donor.
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. Once opened, the solution should be stored at 4°C. This kit is stable for 2 years after the date of shipment.
Component
Cat. 17400 (25 preps)
Cat. 52300 (10 preps)
Cat. 21600 (4 preps)
Wash Solution C
60 mL
60 mL
130 mL
Binding Buffer A
4 mL
4 mL
8 mL
Elution Buffer C
8 mL
30 mL
30 mL
Protein Neutralizer
4 mL
4 mL
4 mL
Micro Spin Columns
25
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–
Midi Spin Columns (assembled with collection tubes)
–
10
–
Maxi Spin Columns (assembled with collection tubes)
Propargyl-PEG4-CH2CO2-NHS has a propargyl group and an NHS group. This reagent is amine reactive, thus, useful for derivatizing biomolecules with amine group. The propargyl group reacts with azides via copper catalyzed azide-alkyne Click Chemistry to form stable triazole bond. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
Propargyl-PEG4-CH2CO2-NHS has a propargyl group and an NHS group. This reagent is amine reactive, thus, useful for derivatizing biomolecules with amine group. The propargyl group reacts with azides via copper catalyzed azide-alkyne Click Chemistry to form stable triazole bond. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
This kit features a rapid, high-throughput 96-well format for isolating high yield and high quality DNA from saliva. The purified saliva DNA is compatible with any number of downstream applications including PCR, qPCR, Southern Blot analysis, sequencing, microarray analysis and more. This kit is also compatible with Norgen’s Saliva DNA Collection and Preservation Devices (Cat. RU49000). It is fully compatible to both vacuum and centrifugation protocols.
Background
Human genomic DNA found in saliva can be used in various applications in diagnostics including the detection of biomarkers to diagnose a disease, follow the disease’s progress or monitor the effects of a particular treatment. Isolation of DNA from saliva for diagnostics has become an attractive alternative to isolation from blood or tissue due to the fact that sample collection is non-invasive.
Storage Conditions and Product Stability The kit contains ready-to-use Proteinase K which is dissolved in a specially prepared storage buffer. The Proteinase K should be stored at room temperature or 4°C. All other solutions should be kept tightly sealed and stored at room temperature (15 – 25°C). This kit is stable for 1 year after the date of shipment.
