Usages: For selective cultivation of bacteria especially Vibrio.
Principle: Peptone provide carbon and nitrogen sources; sodium chloride to maintain osmotic equilibrium; higher pH can suppress the growth of coliform and other bacteria, it is conducive to the growth of Vibrio cholerae.
How to use: 1.Suspend 18g in 1L of distilled water, stirring heated to boiling to completely dissolve, autoclave at 121℃ for 15 minutes. 2.Diluted and treated samples.
Quality control:
Item
The name and number of strain
Growth
Colony Color
1
Vibrio cholerae VbO
Good
Turbid broth
2
Escherichia coli ATCC25922
Poor
—
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
Specifications: 250g/bottle
Other Products
D3182D HiPure Circulating DNA Midi Spin Kit D (Spin Protocol)
Product Info
Document
Product Info
Introduction
Free-circulating nucleic acids, such as tumor-specific extracellular DNA fragments and mRNAs in the blood or fetal nucleic acids in maternal blood, are present in serum or plasmausually as short fragments, <1000bp(DNA). HiPure Circulating DNA Midi Kit enables efficient purification of these circulating nucleic acids from human plasma, serum, or urine. The extracted products can be used for clinical in vitro detection.
Details
Specifications
Features
Specifications
Main Functions
Isolation circulating DNA from 1-5ml plasma, serum, bodyfluids by spin
Applications
qPCR, liquid or solid chip analysis, hybridization and SNP detection, etc.
Purification method
Mini spin column
Purification technology
Silica technology
Process method
Manual (centrifugation)
Sample type
Serum, plasma and other cell-free fluid samples
Sample amount
1-5ml
Elution volume
≥50μl
Time per run
≤70 minutes
Liquid carrying volume per column
4ml
Binding yield of column
1mg
Principle
This product is based on silica gel purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption plate and filter column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10 Mm Tris,pH 8.0).
Advantages
High yield – most optimal process, free DNA (>50bp) can be obtained to the maximum extent
High concentration – low elution volume, ensuring high nucleic acid concentration
High purity – low alcohol binding method, completely removing inhibitor and protein pollution
High recovery – DNA can be recovered at thelevel of PG by silica gel column purification
Kit Contents
Contents
D318203D
Purification Times
50 Preps
Buffer ACL
250 ml
Buffer ACB*
300 ml
Buffer DCW1*
22 ml
Buffer DCW2*
10 ml
Proteinase K
540 mg
Protease Dissolve Buffer
30 ml
Carrier RNA
110 μg
Nuclease Free Water
20 ml
HiPure CFDNA Mini Columns
50
2 ml Collection Tubes
100
Extender Tubes
50
Sealing O-Ringes
50
50ml Collection Tube
50
Support Tube
50
Storage and Stability
Proteinase K, Carrier RNA should be stored at 2-8°C upon arrival. However, short-term storage (up to 12weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can bestored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.
Document
Free-circulating nucleic acids, such as tumor-specific extracellular DNA fragments and mRNAs in the blood or fetal nucleic acids in maternal blood, are present in serum or plasmausually as short fragments,
Alkyne-ethyl-PEG1-t-Butyl ester has an alkyne group and a t-butyl protected carboxyl group. The alkyne reacts with azide compounds or biomolecules via copper catalyzed Click Chemistry to form a stable triazole linkage. Thecarboxyl group group can be deprotected under mild acidic conditions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
Alkyne-ethyl-PEG1-t-Butyl ester has an alkyne group and a t-butyl protected carboxyl group. The alkyne reacts with azide compounds or biomolecules via copper catalyzed Click Chemistry to form a stable triazole linkage. Thecarboxyl group group can be deprotected under mild acidic conditions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.