For enrichment of bacteria of cosmetics and disposable hygiene products.
Principle:
Casein peptone and soybean peptone provide the nitrogen source, vitamins, and growth factors; sodium chloride to maintain osmotic balance; dipotassium hydrogenphosphate as a buffer; lecithin and Tween 80 can neutralize preservatives and can play a dispersed material in the emulsion features, lecithin can neutralize quaternary ammonium salt, Tween 80 neutralizes phenols, hexachlorophene, formalin, a combination that can neutralize and ethanol.
Formulation(per liter):
Casein peptone 17g
Soy peptone 3g
Sodium chloride 5g
Dipotassium hydrogen phosphate 2.5g
Glucose 2.5g
Lecithin 1g
Tween 80 7g
Final pH7.2 ± 0.2
How to use:
1.Suspend 38g in 1 L of distilled water , stirring heated to boiling until completely dissolved, distribute into flask,autoclave at 121 for 15 minutes.
2.Diluted and treated samples.
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
This product provides a reliable solution for DNA isolation from yeast samples. Total DNA can be purified from yeast (<5x 107) without phenol or chloroform. The whole extraction can be finished within 60 minutes. Purified DNA can be directly used for PCR, Southern blot, ect.
Details
Specifications
Features
Specifications
Main Functions
Isolation total DNA from yeast cultures
Applications
PCR, southern blot and enzyme digestion, etc.
Purification method
Mini spin column
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
Yeast culture
Sample amount
Bacterial culture: 1-1.5 ml
Elution volume
≥30μl
Time per run
≤60 minutes
Liquid carrying volume per column
800μl
Binding yield of column
100μg
Principle
This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mm Tris,pH9.0, 0.5mm EDTA).
Advantages
Fast – several samples can be extracted in 40 minutes (after digestion)
High purity – purified DNA can be directly used in various downstream applications
Good repeatability – silica technology can obtain ideal results every time
High recovery – DNA can be recovered at the level of PG
Sufficient components – lysozyme, protease K, RNase A and glass beads
Kit Contents
Contents
D314702
D314703
Purification Times
50 Preps
250 Preps
Hipure DNA Mini Columns I
50
250
2ml Collection Tubes
50
250
Glass Beads (0.4~0.6mm)
20 g
90 g
Buffer SE
30 ml
150 ml
Lyticase
1.8 ml
5 x 1.8 ml
Buffer ATL
30 ml
150 ml
ReagentDX
500 μl
1500 μl
Buffer DL
30 ml
150 ml
Buffer GW1*
13 ml
66 ml
Buffer GW2*
20 ml
2 x 50 ml
Proteinase K
12 mg
60 mg
Protease Dissolve Buffer
1.8 ml
5 ml
Buffer AE
15 ml
30 ml
Storage and Stability
Proteinase K should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions. Buffer ATL may precipitate at low temperature. Dissolve it by 37℃ water bath.
Document
This product provides a reliable solution for DNA isolation from yeast samples. Total DNA can be purified from yeast (
