For selective isolation and culture of Pseudomonas aeruginosa.
Principle:
Acetamidase bacteria can use acetamide as a carbon source and acetamide decomposition Alcaligenes; NaCl maintain osmotic balance; dipotassium phosphate and potassium dihydrogenphosphate as buffer; magnesium ions provide necessary growth ; agar medium as coagulant; phenol red as a pH indicator.
Formulation:
Acetamide 10g
NaCl 5g
Dipotassium hydrogen phosphate 1.39g
Potassium dihydrogen phosphate 0.73g
Magnesium sulfate 0.5g
Phenol red 0.012g
Agar 15g
Final pH 7.2 ± 0.2
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
Other Products
Total RNA Purification Kits
Product Info
Document
Product Info
Overview
Extract high quality & quantity total RNA including miRNA
No phenol step required; isolate all RNA in one fraction
Bind & elute all RNA irrespective of size or GC content, without bias
Very sensitive & linear down to a few cells without the need for carrier RNA
Isolate from a wide variety of specimens
Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen’s NGS services
Available in a variety of formats to suit your needs
Purification is based on spin column chromatography that uses Norgen’s resin separation matrix
These kits are suitable for the isolation of total RNA from a range of samples including cells, bacteria, yeast, virus and bodily fluids including plasma/serum, blood, saliva, CSF and more. Extract high quality and purity RNA with excellent RIN values and A260/A280 suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS and more. These kits purify all sizes of RNA from large mRNA, lncRNA down to microRNA (miRNA) in the same fraction without the requirement of phenol. Isolate all RNA sequences at an equal rate irrespective of size. Moreover, when the RNA sequences are small (e.g. miRNA), the column binds small RNAs regardless of their GC content.
Total RNA Purification 96-Well Kit (High Throughput and High Throughput Deep Well)
This 96-well kit provides a rapid method for the high-throughput isolation and purification of total RNA in 30 minutes using vacuum manifold, plate centrifuge, or liquid handlers with vacuum capabilities. Total RNA can be isolated from a broad range of sample sources including cultured cells, tissues, blood, serum, plasma, bacteria, yeast, fungi, and viruses.
* for isolating total RNA from larger amounts of tissue, please use Norgen’s Animal Tissue RNA Purification Kit (Cat# 25700)
Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. These kits are stable for 2 years after the date of shipment.
Produced by reverse osmosis, ion-exchange, electro-deionization, ultrafiltration, UV treatment (UVC & 185 nm), 0.1 μm membrane filtration
Nuclease-free, protease-free, endotoxin-free
Suitable for molecular biology applications (qPCR, NGS etc.)
Suitable for cell culture applications
Produced in an ISO certified GMP laboratory
Water system is routinely monitored for compliance with QC standards
The Norgen water purification system has been designed in conjunction with experts from Millipore Sigma. The Ultrapure NFW is purified in a two-stage process. The first stage, to produce type II water, includes pre-treatment with activated charcoal and polyphosphate, this water then undergoes reverse osmosis, electro-deionization and ion exchange to remove contaminants as well as UV treatment for disinfection. The Pure water then flows to a second system in a clean room with an additional 4 layers of purification. After passing through an additional ion exchange column, the water is then subjected to Ultrafiltration to remove particulates, bacteria, & ionic contaminants down to trace level, the water is then treated with UV light (182 nm wavelength) to break down organic matter. Lastly a second Ultrafilter for the production of pyrogen-, nuclease- and bacteria-free water at 18.2 MΩ. This water is of utmost purity and suitable for direct use in molecular biology applications such as qPCR and NGS, as well as cell culture and other applications.
Product Specifications
pH
n/a (highly pure water does not contain enough ions for an exact pH determination.)
Recommended Storage
Room Temperature
Grade
Ultrapure (ASTM Type I)
Biological Activity
DNase-, RNase-, Protease-, Endotoxin- Free
TOCs
< 50 ppb
Resistivity
> 18 MΩ-cm
Treatment
Not DEPC-Treated
Quantity
100 mL, 500 mL, 1000 mL
Purification Methods
Activated charcoal, polyphosphate, reverse osmosis, electro-deionization, ion exchange, UV, ultrafiltration
Shipping Conditions
Room Temperature
Details
Applications For use in any molecular biology application
Storage Conditions Norgen’s Nuclease-Free Water should be stored at room temperature. Storage at +4°C or -20°C is optional.
Precautions and Disclaimers This product is designed for research purposes only. It is not intended for human or diagnostic use.
PEG3-(Amino-Tri-(Propargyl-PEG2-ethoxymethyl)-methane)-(Amino-Tri-(carboxyethoxymethyl)-methane) is reactive with azide-bearing compounds or biomolecules via copper catalyzed azide-alkyne Click Chemistry to yield a stable triazole linkage. The terminal carboxylic acid groups can react with primary amino groups in the presence of activators (e.g. HATU) to form a stable amide bond.
Document
PEG3-(Amino-Tri-(Propargyl-PEG2-ethoxymethyl)-methane)-(Amino-Tri-(carboxyethoxymethyl)-methane) is reactive with azide-bearing compounds or biomolecules via copper catalyzed azide-alkyne Click Chemistry to yield a stable triazole linkage. The terminal carboxylic acid groups can react with primary amino groups in the presence of activators (e.g. HATU) to form a stable amide bond.
