How to use： 1.Suspend 38g in 1 L of distilled water , stirring heated to boiling until completely dissolved, dispensing flask, 121 ℃ autoclave for 15min. 2.Diluted and treated samples.
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
Cytokeratin 19 (CK19) forms intermediate filaments found in the intracytoplasmic cytoskeleton of epithelial tissue and provides mechanical support. Anti-Cytokeratin 19 stains epithelia and epithelial malignancies such as carcinomas of the colon, stomach, pancreas, biliary tract, liver, and breast. Cytokeratin 19 is a useful marker for distinguishing hepatocellular carcinoma from intrahepatic cholangiocarcinoma. This differentiation is improved when stained in combination with Cytokeratin 7, CAM5.2l, Ber-EP4/MOC31, HepPar1 and TTF1. Cytokeratin 19 staining can also be used to recognize thyroid papillary carcinomas.
1.5 mL – 2 mL Micro-centrifuge Tube or Screw-Cap Vial Chiller Block
SKU: CB024
Permagen’s SBS/ANSI footprint 24-Tube thermal block allows the use of up to twenty four 1.5 mL -2 mL Microcentrifuge tubes, or screw-cap vials for keeping sample temperatures consistent. Can be used for heating as well
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Permagen’s SBS/ANSI footprint 24-Tube thermal block allows the use of up to twenty four 1.5 mL -2 mL Microcentrifuge tubes, or screw-cap vials for keeping sample temperatures consistent. Can be used for heating as well
The Kit is specially designed for simultaneous purification of genomic DNA and total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue sections. Purified DNA/RNA is suitable for use in applications such as real-time PCR and Pyrosequencing.
Details
Specifications
Features
Specifications
Main FunctionsC
Co-isolation total RNA and DNA from FFPE tissue
Applications
RT-PCR, cDNA synthesis, PCR and second-generation sequencing, etc.
Purification method
Polydisperse magnetic beads
Purification technology
Magnetic beads technology
Process method
Manual or automatic
Adaptive instrument
Nucleic acid extractor and pipetting workstation
Sample type
FFPE slice, FFPE puncture sample, embedded tissue
Sample amount
No more than six 10µm sections of 150 mm2 surface area or three 20µm sections of 150 mm2 surface area.
Principle
FFPE samples are incubated in an optimized lysis buffer, which results in the release of RNA and precipitation of DNA. After centrifugation, the RNA-containing supernatant and DNA-containing pellet are then processed separately to purify RNA and DNA. After adding magnetic particles and binding solution, DNA/RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA was eluted by RNase Free Water.
Advantages
Post digestion sorting – higher DNA and RNA yields
Economy – the price is much lower than imported reagents
Kit Contents
Contents
IVD3026
Purification Times
200 Preps
MagBind Particles
9.0 ml
Proteinase K
180 mg
Protease Dissolve Buffer
10 ml
Buffer DPS
150 ml
Buffer FRL
40 ml
Buffer ATL
40 ml
Buffer AL
80 ml
Buffer BXW1*
110 ml
RNase Free Water
30 ml
Storage and Stability
Proteinase K and MagBind Particles should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
Document
The Kit is specially designed for simultaneous purification of genomic DNA and total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue sections. Purified DNA/RNA is suitable for use in applications such as real-time PCR and Pyrosequencing.
