How to use： 1.Suspend 38g in 1 L of distilled water , stirring heated to boiling until completely dissolved, dispensing flask, 121 ℃ autoclave for 15min. 2.Diluted and treated samples.
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
Specifications: 250g/bottle
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EXTRAClean Plasma/Serum Exosome and Free-Circulating RNA Isolation Kit
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Overview
Ensure optimal results for sensitive applications like NGS
Up to a tenfold increase in microRNA mapping during sequencing runs to reduce costs
The purified exosomal RNA is free from any circulating RNA-binding proteins.
No phenol extractions, Proteinase K treatment, nor carrier RNA.
No time-consuming ultracentrifugation, filtration nor special syringes are required.
Concentrate isolated RNA into a flexible elution volume ranging from 50 μL to 100 μL.
Purification is based on EXTRAClean spin column chromatography that uses Norgen’s proprietary separation matrix.
Norgen’s EXTRAClean Plasma/Serum Exosome and Free-Circulating RNA Isolation Kit constitutes an all-in-one system for the purification of exosomes and the sequential isolation of RNA and free-circulating RNA from different plasma/serum sample volumes ranging from 50 μL and up to 10 mL. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. The EXTRAClean columns undergo stringent processing and rigorous quality control measures to minimize contamination traces, ensuring optimal results for sensitive applications such as NGS. The kit is designed to isolate all sizes of RNA, including microRNA. The kit provides a clear advantage over other available kits in that they do not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or any protease treatments. Moreover, the kit allows the user to elute into a flexible elution volume ranging from 50 μL to 100 μL. The RNA isolated from the purified exosomes is free from any protein-bound circulating RNA and is of the highest integrity. Moreover, the free-circulating, protein-bound, RNA is free from any exosomal RNA. The purified RNA can be used in a number of downstream applications including real time PCR, NGS application, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.
All sizes, including miRNA and small RNA (< 200 nt)
Elution Volume
50-100 μL
Time to Complete 10 Purifications
35-40 minutes
Average Yields
Variable depending on specimen
Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.
96-Well Low Elution Ring Magnetic Plate with Integrated Cushion Base
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Designed for those labs wishing to separate magnetic beads into rings.
The Permagen ring magnet low elution plate with integrated cushion base plate was designed for use in automation applications where volumes as low as 5 µL are required. Most PCR plates are bent, leading to inconsistent lab results. Unlike most products, we have added an angled frame around the top of our plate, this helps with two things, straightening out the PCR plate, and leading it to the proper location on the magnets during automation while the cushion assist compensates for labware inconsistencies.
SBS SLAS Footprint (127.75mm x 85.50mm) to fit into any automated liquid handling robot
Features include solid aluminum alloy construction and hard coat anodized finish for years of trouble-free use, and compatible with any magnetic beads
Designed for those labs wishing to separate magnetic beads into rings.
The Permagen ring magnet low elution plate with integrated cushion base plate was designed for use in automation applications where volumes as low as 5 µL are required. Most PCR plates are bent, leading to inconsistent lab results. Unlike most products, we have added an angled frame around the top of our plate, this helps with two things, straightening out the PCR plate, and leading it to the proper location on the magnets during automation while the cushion assist compensates for labware inconsistencies.
Propargyl-PEG14-alcohol is a propargyl linker which enables Click Chemistry with azide compounds to yield stable triazole linkage. The PEG spacer enhances the hydrophilicity of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
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Propargyl-PEG14-alcohol is a propargyl linker which enables Click Chemistry with azide compounds to yield stable triazole linkage. The PEG spacer enhances the hydrophilicity of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
