029010 Gram Stain

Overview

• Ready-to-use
• Quantitative
• Highly Stable
• Precise
• Ten discrete fragments ranging from 300 bp to 5000 bp

The Norgen MidRanger 1 kb DNA Ladder is prepared to ensure quality and batch-to-batch consistency. Our MidRanger contains ten discrete fragments ranging from 300 bp to 5000 bp. This Ladder is ideal for sizing larger PCR products (>1kb) and most cloning applications.

Contents:
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).

Details

Supporting Data

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MidRanger 1kb DNA Ladder (Cat# 11700) – 100 loads

Ladder Properties:
• Ten discrete fragments ranging from 300 bp to 5000 bp

Fragment	Size (bp)	Mass (ng)
1	5000	88
2	4000	78
3	3000	67
4	2500	58
5	2000	50
6	1500	43
7	1000	25
8	700	34
9	500	33
10	300	24

Recommended Use:

Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.

Storage: 

Stable at room temperature. For longer term storage, -20°C is recommended.

This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.

DBCO-PEG6-amine is a PEG linker which contains DBCO and amine moieties. The DBCO group is commonly used in copper-free Click Chemistry reactions. The amine group is reactive with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde) etc. The hydrophilic PEG spacer increases the water solubility of the compound. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

DBCO-PEG6-amine is a PEG linker which contains DBCO and amine moieties. The DBCO group is commonly used in copper-free Click Chemistry reactions. The amine group is reactive with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde) etc. The hydrophilic PEG spacer increases the water solubility of the compound. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

RevTaq RT-PCR DNA polymerase is an engineered, extremely thermostable reverse transcriptase and combined DNA polymerase, obtained through directed, artificial evolution.

RevTaq RT-PCR DNA polymerase can be used similarly to Tth DNA polymerase also for reverse transcription, but does not require the addition of Mn2+ for RT-PCR, which simplifies assay setup and possible sample processing.

– half-life at 95°C of >40 min.

– fast start function due to a hotstart aptamer formulation which prevents unspecific amplification at lower temperatures (<57°C)

– facilitates “zero-step” RT-PCRs directly from RNA templates (without an isothermal reverse transcription step), as reverse transcription takes place simultaneously with DNA amplification during the cycled PCR elongation step.

– allows reverse transcription reactions at high temperatures, thus minimizing the problems encountered with strong secondary structures in RNA that melt at elevated temperatures.

– RevTaq RT-PCR DNA polymerase is the pure, reverse transcription active enzyme ingredient of our Volcano3G® RT-PCR Master Mixes

Please note: Due to the thermostable nature of the enzyme, it is recommended to design very high melting primers and probes (>65°C). See more details in FAQ.

For research use and further manufacturing.

In case you are aiming to use our RUO products as components or for your development of e.g. an IVD medical device, please contact us.

RevTaq RT-PCR DNA polymerase is an engineered, extremely thermostable reverse transcriptase and combined DNA polymerase, obtained through directed, artificial evolution.

RevTaq RT-PCR DNA polymerase can be used similarly to Tth DNA polymerase also for reverse transcription, but does not require the addition of Mn2+ for RT-PCR, which simplifies assay setup and possible sample processing.

– half-life at 95°C of >40 min.