
Introduction
029020 Spore Stain
Usage:For Spore Stain Test.
Specification:10ml*2vials
10ml*2vials
029020 Spore Stain
Usage:For Spore Stain Test.
Specification:10ml*2vials
Clone | IHC402 |
Source | Mouse Monoclonal |
Positive Control | Colon |
Dilution Range | 1:200 |
CDX-2 is a caudal-related homeobox transcription factor that is expressed by intestinal epithelial cells. CDX-2 is a useful marker for gastrointestinal carcinoma, and for determining the origin of gastrointestinal metastatic adenocarcinoma and carcinoids. Anti-CDX-2 is used for differentiating lung and metastatic colorectal adenocarcinoma, however mucinous ovarian carcinoma also react positively with Anti-CDX-2, thereby limiting the ability to differentiate from metastatic colorectal adenocarcinoma.
Product Description
Long-Term Stable Nucleic Acid Reagent For RNA Extraction
Product Description:
Amp-future Bio’s Nucleic Acid Extraction Reagent is a RNA extraction kit that provides the highest quality nucleic acid extraction. The RNA extraction kit is an automated nucleic acid extractor which allows for quick and easy RNA isolation. The reagent is in liquid form and has a shelf life of 12 months, making it a reliable and durable product. With the Nucleic Acid Extraction Reagent, Amp-future Bio provides the accuracy and reliability of a professionally designed RNA extraction kit.
Features:
Technical Parameters:
Property | Value |
---|---|
Form | Liquid |
Product Name | RNA Nucleic Acid Extraction Reagent |
Application | RNA Extraction |
Brand | Amp-future Bio |
Shelf Life | 12 Months |
Composition | 5ml*4 tubes |
Applications:
Amp-future Bio’s Nucleic Acid Reagent is an advanced automated nucleic acid extraction solution designed to efficiently extract RNA from biological samples while maintaining quality and accuracy. It is made in China and has a 12-month shelf life. It comes in a liquid form and is suitable for use in automated nucleic acid extractors, allowing for automatic and highly efficient extraction of nucleic acid from various sample types. The reagent is also designed to be easy to use, making it an ideal solution for laboratories that require fast and reliable results. This reagent is a great choice for any research or clinical laboratory that needs efficient and accurate nucleic acid extraction.
Support and Services:
Technical Support and Services for Nucleic Acid Reagent
We provide technical support and services for Nucleic Acid Reagents. Our experts are available to help you identify the right reagents for your application, answer any questions, and provide assistance on the use and optimization of these reagents. Our experts are also available to provide advice on troubleshooting and protocol optimization. We offer a range of services to make ordering and stocking of reagents easy and convenient.
We provide:
Please contact us for more information about our technical support and services for nucleic acid reagents.
The HiPure Minipreps system provides a fast, simple, and cost-effective plasmid DNA miniprep method for routine molecular biology laboratory applications. HiPure Miniprep Kits use silica membrane technology to eliminate the cumbersome steps associated with loose resins or slurries. Plasmid DNA purified with Mini Kits is immediately ready for use. Phenol extraction and ethanol precipitation are not required, and high quality plasmid DNA is eluted in a small volume of Tris buffer or water.
Specifications
Features | Specifications |
Main Functions | Isolation up to 30μg endotoxin-free plasmid DNA from 1-5ml bacterial culture using 96-well bind plate |
Applications | Cell transfection, animal injection, etc. |
Purification method | 96 well plate |
Purification technology | Silica technology |
Process method | Manual (centrifugation or vacuum) |
Sample type | Low copy plasmid vector |
Sample amount | 1-5ml LB(x96) |
Yield | 30μg |
Elution volume | ≥75μl |
Time per run | ≤60 minutes |
Liquid carrying volume per column | 800μl |
Binding yield of column | 70μg |
The HiPure Plasmid procedure is based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt. The unique silica membrane used in the kit completely replaces glass or silica slurries for plasmid DNA minipreps. The procedure consists of 3 basic steps: Preparation and clearing of a bacterial lysate by alkaline method,then transfer the supernatant to column to bind DNA. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
Advantages
1. High throughput: processing 96 samples at once;
2. High yield: The purified plasmid yield can reach up to 30μg, which is greater than the yield of the magnetic bead method kit;
3. Detoxification: endotoxin content <0.1EU/μg;
4. Fast: The entire extraction can be completed in 60 minutes without the need for time-consuming alcohol precipitation;
Kit Contents
Contents | P115701 | P115702 | P115703 |
Purification Times | 1 x 96 Preps | 4 x 96 Preps | 20 x 96 Preps |
RNase A | 10 mg | 20 mg | 100 mg |
Buffer P1 | 30 ml | 120 ml | 550 ml |
Buffer P2 | 30 ml | 120 ml | 550 ml |
Buffer LEN3 | 15 ml | 60 ml | 300 ml |
Buffer LN4 | 80 ml | 270 ml | 2 x 700 ml |
Buffer LN5 | 40 ml | 220 ml | 1100 ml |
Buffer PW1 | 40 ml | 220 ml | 1100 ml |
Buffer PW2 | 25 ml | 100 ml | 2 x 200 ml |
Elution Buffer | 30 ml | 60 ml | 250 ml |
Lysate Clear Plate | 1 | 4 | 20 |
HiPure DNA Plate | 1 | 4 | 20 |
1.6ml Collection Plate | 2 | 8 | 40 |
0.5ml Collection Plate | 1 | 4 | 20 |
Storage and Stability
The kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve. After addition of RNase A,Buffer P1 is stable for 6 months when stored at 2-8°C.
For any technical problems or customized products, please contact us.
F&Q about Endotoxin-free Plasmid Extraction Kit — P1156 ←click here
The HiPure Minipreps system provides a fast, simple, and cost-effective plasmid DNA miniprep method for routine molecular biology laboratory applications. HiPure Miniprep Kits use silica membrane technology to eliminate the cumbersome steps associated with loose resins or slurries. Plasmid DNA purified with Mini Kits is immediately ready for use. Phenol extraction and ethanol precipitation are not required, and high quality plasmid DNA is eluted in a small volume of Tris buffer or water.
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Tel : 081-875-1869 , 02-328-7179
Email : hej@a3p-scientific.com
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