
Introduction
029020 Spore Stain
Usage:For Spore Stain Test.
Specification:10ml*2vials
10ml*2vials
029020 Spore Stain
Usage:For Spore Stain Test.
Specification:10ml*2vials
Listeria monocytogenes have emerged as significant foodborne pathogens that pose a serious public health problem. As the causative agent of Listeriosis, L. monocytogenes has the highest rate of fatality rate among all foodborne pathogens. L. monocytogenes is a facultatively intracellular, Gram-positive bacterium that could survive high and low temperatures, low pH. It is a rare causative agent of mastitis in cow. However, due to its ability to resist various food processing technologies as well as to grow at low temperature, L. monocytogenes is know to be associated with both raw and pasteurized milk, as well as dairy products such as cheese. As little as 1000 organisms may cause the disease with pregnant, new-born, and immunocompromised individuals the most susceptible.
Listeria monocytogenes TaqMan PCR Kit, 24 reactions
Figure 1 / 3
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Storage Conditions
The Listeria monocytogenes TaqMan PCR Kit Dx is shipped on dry ice. The components of the kit should be frozen upon arrival. If one or more of the components is not frozen when the kit is received, or if any of the components have been compromised during shipment, please contact Norgen Biotek for assistance. All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 3 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
Component | Cat. DxTM30400 (24 rxns) |
---|---|
MDx TaqMan 2X PCR Master Mix Dx | 550 µL |
L. monocytogenes Primer Mix Dx | 2 x 70 µL |
L. monocytogenes Positive Control Dx – 200,000 copies/μL | 50 µL |
Nuclease-Free Water | 2 x 1.25 mL |
Product Insert | 1 |
Features
1. Widely used in the field of pharmaceutical factory and laboratory.
2. Brushless frequency motor, in great torque, free maintenance, no powder pollution, quick in speed up and down.
3. Digital display which indicates the program, speed, time,RCF, temperature.
4. Micro computer control, there are 10 kinds of program and 10 kinds of acceleration and deceleration for your choice.
5. Several rotors for your choice.
6. Electric lid lock, compact design, super speed and imbalance protection.
7. The centrifuge body is made of high-quality steel, safe and reliable.
GL10 Technical Parameter:
Max. Speed | 10000rpm |
Max. RCF | 15730×g |
Max. Capacity | 6×1000ml |
Time Range | 1~9h59min |
RPM/RCF Convert | Yes |
Noise (dB) | ≤ 65 |
Temperature | -20~40℃ |
Acc/Dec | 10 Kinds |
Speed Accuracy | ±20r/min |
Temperature Accuracy | ±1℃ |
Voltage(V/Hz) | AC 380V/220V 50HZ/60HZ |
Size (W x D x Hmm) | 800×740×930mm |
Net Weight(Kg) | 360KG |
Certificates | CE,ISO & Calibration report are available |
Matched Rotors for GL10
Order No. | Rotor No. | Max Speed (rpm) | Max Volume(ml) | Max.RCF(×g) |
G10-1 | Angle Rotor | 8000 | 6×1000ml | 14550 |
G10-2 | Angle Rotor | 9000 | 6×500ml | 14860 |
G10-3 | Angle Rotor | 10000 | 6×300ml | 15730 |
G10-4 | Swing Rotor | 4200 | 6×1000ml | 5100 |
MagZol Reagent is a reagent system for the isolation of total RNA from cells and tissues. The reagent, a single-phase solution consisting of phenol and guanidine isothiocyanate, is modification of the single-step RNA isolation method developed by Chomczynski and Sacchi. The sample is homogenized and lysed in MagZol Reagent which maintains the integrity of the RNA, while disrupting and denaturing endogenous RNases and other cellular components. Extraction of the lysate with chloroform further denatures proteins and separates the mixture into an organic and an aqueous phase. RNA remains exclusively in the aqueous phase, and is subsequently recovered by isopropanol.
This method is suitable for small quantities of tissue (<100mg) and cells (<5 X106), and large quantities of tissue (up to 1g) and cells (<108), of human, animal, plant, or bacterial origin. The simplicity of the MagZol Reagent method allows simultaneous processing of a large number of samples. The entire procedure can be completed in one hour. Total RNA prepared in this manner can be used for Northern blot analysis, dot blot hybridization, poly(A) + selection, in vitro translation, RNase protection assay, and molecular cloning. For use in amplification by thermal cycling, treatment of the isolated RNA with RNase-free DNase I is recommended when the two amplimers lie within a single exon.
Specifications
Features | Specifications |
Main Functions | Extract RNA from liquid samples by salting out method |
Applications | RT-PCR, Northern hybridization, poly (a) enrichment, etc. |
Purification technology | Acid phenol guanidine isothiocyanate |
Process method | Manual (centrifugation) |
Sample type | Various liquid samples |
Sample amount | Flexible |
Elution volume | Variation with sample size |
Time per run | Variation with sample size |
Storage and Stability
MagZol Reagent should be stored at 2-8°C upon arrival and is stable for at least 24 months under the condition. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect its performance.
MagZol Reagent is a reagent system for the isolation of total RNA from cells and tissues. The reagent, a single-phase solution consisting of phenol and guanidine isothiocyanate, is modification of the single-step RNA isolation method developed by Chomczynski and Sacchi. The sample is homogenized and lysed in MagZol Reagent which maintains the integrity of the RNA, while disrupting and denaturing endogenous RNases and other cellular components. Extraction of the lysate with chloroform further denatures proteins and separates the mixture into an organic and an aqueous phase. RNA remains exclusively in the aqueous phase, and is subsequently recovered by isopropanol.
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