
Introduction
029070 Nitrate Reduction Reagent
Usage:For Nitrate Reduction Test.
Specification:10ml*2vials
10ml*2vials
029070 Nitrate Reduction Reagent
Usage:For Nitrate Reduction Test.
Specification:10ml*2vials
This kit provides a fast, reliable and convenient method to isolate and concentrate exosomal RNA from previously purified exosomes. It allows for the isolation of RNA from intact extracellular vesicles (EVs) purified from different urine or plasma/serum sample volumes. The purification is based on Norgen’s proprietary resin.
The Exosomal RNA Isolation Kit is designed to isolate all sizes of RNA, including microRNA. Moreover, the kit allows the user to elute into a flexible elution volume ranging from 50 µL to 100 µL. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.
Figure 1 / 2
Click for expanded view
Kit Specifications | |
Sample type | Exosomes purified using Norgen’s Purification Kits and most other methods |
Size of RNA Purified | All sizes, including miRNA and small RNA (< 200 nt) |
Elution Volume | 50-100 μL |
Time to Complete 10 Purifications | 35-40 minutes |
Average Yields* | Variable depending on specimen |
*Please check page 5 of the product insert for the average yields and the common RNA quantification methods.
Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.
Component | Cat. 58000 (50 preps) |
---|---|
Lysis Buffer A | 20 mL |
Lysis Additive B | 2 mL |
Wash Solution A | 18 mL |
Elution Solution A | 6 mL |
Mini Spin Columns | 50 |
Collection Tubes | 50 |
Elution Tubes (1.7 mL) | 50 |
Product Insert | 1 |
Norgen’s Swab Collection and DNA Preservation System is designed for collection, ambient storage and transport of DNA from samples collected using a swab, including nasal, buccal, saliva, fecal, skin, surfaces, etc. The Swab Collection and DNA Preservation System contains Norgen’s Swab Preservative in a liquid format. The user simply collects the specimen using the provided swab, and then transfers the swab into the Swab Preservative. The Swab Preservative prevents the growth of Gram-negative and Gram-positive bacteria and fungi, and also inactivates viruses allowing the resulting non-infectious samples to be handled and shipped safely. In addition, the Swab Preservative eliminates the need to immediately process or freeze samples and allows the samples to be shipped to centralized testing facilities at ambient temperatures. The components of the Swab Preservative allow DNA samples to be stored at room temperature for over 2 years.
DNA Isolation from Preservative
Swab samples collected and preserved using Norgen’s Swab Collection and DNA Preservation System are compatible with most DNA isolation methods. Samples stored in the tubes have been used successfully with most of Norgen Biotek’s DNA isolation kits and reagents. An optimized isolation procedure is available as part of Norgen’s Microbiome DNA Isolation Kit (Cat. 64100).
Figure 1 / 4
Click for expanded view
Kit Specifications | |
Volume of Preservative | 1.2 mL |
Preservation Temperature | Room Temperature |
Preservation time | 2 years at room temperature |
Shelf Life and Handling
When stored at room temperature, unused Norgen Swab Preservative Tubes are stable through to the collect-before date without any reduction in performance. Please see the device insert or tube label for collect-before date.
Kit Components | Cat. 45690 (50 units) |
---|---|
Swab Collection and DNA Preservation Kit | 50 |
Individually Wrapped Swabs | 50 |
Product Insert | 1 |
The HiPure Plasmid DNA Plus 96 Kit enables up to 96 minipreps to be performed simultaneously in less than 45 minutes on the Vacuum Manifold (Qiavac 96). This kit provides a fast, simple,and cost-effective plasmid DNA high-throughput method for routine molecular biology laboratory applications. HiPure Kits use silica membrane technology to eliminate the cumbersome steps associated with loose resins or slurries.Plasmid DNA purified with Kits is immediately ready for use. Phenol extraction and ethanol precipitation are not required, and high quality plasmid DNA is eluted in a small volume of Tris buffer or water.
Specifications
Features | Specifications |
Main Functions | Isolation up to 20µg plasmid DNA from 1.5ml bacterial culture using 96 well bind plate and 96 filterplate |
Applications | Enzyme digestion, sequencing, PCR, labeling, etc. |
Purification method | 96 well plate |
Purification technology | Silica technology |
Process method | Manual (centrifugation or vacuum) |
Sample type | Conventional plasmid, plasmid less than 30KB |
Sample amount | 1-1.5ml(x96) |
Yield | 1-15µg/1ml |
Elution volume | ≥70μl |
Time per run | ≤60 minutes |
Liquid carrying volume per column | 800µl |
Binding yield of column | 70µg |
The HiPure Plasmid procedure is based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt. The unique silica membrane used in the kit completely replaces glass or silica slurries for plasmid DNA minipreps. The procedure consists of 3 basic steps: Preparation and clearing of a bacterial lysate by alkaline method,then transfer the supernatant to column to bind DNA. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
Advantages
Kit Contents
Contents | P100601 | P100602 | P100603 |
Purification Times | 1 x 96 Preps | 4 x 96 Preps | 20 x 96 Preps |
RNase A | 5 mg | 20 mg | 100 mg |
Buffer P1 | 30 ml | 120 ml | 600 ml |
Buffer P2 | 30 ml | 120 ml | 600 ml |
Buffer P3 | 40 ml | 180 ml | 800 ml |
Buffer PW1 | 100 ml | 500 ml | 2 x 1000 ml |
Buffer PW2 | 50 ml | 2 x 100 ml | 4 x 200 ml |
Elution Buffer | 150 ml | 60 ml | 300 ml |
Lysate Clear Plate | 1 | 4 | 20 |
HiPure DNA Plate | 1 | 4 | 20 |
1.6 ml Collection Plate | 1 | 4 | 20 |
0.5ml Elute Plate | 1 | 4 | 20 |
Storage and Stability
The kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve. After addition of RNase A,Buffer P1 is stable for 6 months when stored at 2-8°C.
For any technical problems or customized products, please contact us.
F&Q about Endotoxin-free Plasmid Extraction Kit — P1156 ←click here
The HiPure Plasmid DNA Plus 96 Kit enables up to 96 minipreps to be performed simultaneously in less than 45 minutes on the Vacuum Manifold (Qiavac 96). This kit provides a fast, simple,and cost-effective plasmid DNA high-throughput method for routine molecular biology laboratory applications. HiPure Kits use silica membrane technology to eliminate the cumbersome steps associated with loose resins or slurries.Plasmid DNA purified with Kits is immediately ready for use. Phenol extraction and ethanol precipitation are not required, and high quality plasmid DNA is eluted in a small volume of Tris buffer or water.
83, On-nut 88/2 Prawet Sub-district, Prawet District, Bangkok, 10250, Thailand
Tel : 081-875-1869 , 02-328-7179
Email : hej@a3p-scientific.com
Copyright © 2024 A3P Scientific Co., Ltd. All rights reserved. Web by Mountain Studio
Privacy Policy | Terms of Use | Site Map