
Introduction
029070 Nitrate Reduction Reagent
Usage:For Nitrate Reduction Test.
Specification:10ml*2vials
10ml*2vials
029070 Nitrate Reduction Reagent
Usage:For Nitrate Reduction Test.
Specification:10ml*2vials
Real-time fluorescent DNA amplification in a flexible liquid format. Recommended for users who want to combine RPA with the use of TwistDx’s proprietary fluorescent TwistAmp® exo Probes. In addition to the basic components, a powerful nuclease (Exonuclease III) is provided which will process TwistAmp® exo Probes during the amplification reaction itself and generate a real-time readout. The user need only supply primers, probe, dNTPs and template.See manual for more information. Click to order oligonucleotides.
Perfect for: Real-time fluorescent DNA detection, facilitating use of different RPA reaction volumes, or variation of component ratios.
Product code: TALQEXO01
Real-time fluorescent DNA amplification in a flexible liquid format. Recommended for users who want to combine RPA with the use of TwistDx’s proprietary fluorescent TwistAmp® exo Probes. In addition to the basic components, a powerful nuclease (Exonuclease III) is provided which will process TwistAmp® exo Probes during the amplification reaction itself and generate a real-time readout. The user need only supply primers, probe, dNTPs and template.See manual for more information. Click to order oligonucleotides.
Description
The GetClone™ PCR Cloning Vector II is a positive selection system for high efficiency cloning of blunt end DNA or amplicons. This cloning vector contains a lethal gene which can be disrupted by ligation of a blunt end DNA insert into the cloning site. Only colonies with inserted vectors are able to propagate, eliminating the additional needs of IPTG and X-Gal for blue/white screening. This cloning vector includes ampicillin and kanamycin resistance genes that can meet the needs of most users.
Features
Storage
-20°C for 24 months
The GetClone™ PCR Cloning Vector II is a positive selection system for high efficiency cloning of blunt end DNA or amplicons. This cloning vector contains a lethal gene which can be disrupted by ligation of a blunt end DNA insert into the cloning site. Only colonies with inserted vectors are able to propagate, eliminating the additional needs of IPTG and X-Gal for blue/white screening. This cloning vector includes ampicillin and kanamycin resistance genes that can meet the needs of most users.
This product is suitable for rapid extraction of DNA from plant and fungal samples. The kit is based on silica gel column purification technology. The whole extraction process only takes 60-90 minutes. DNA can be used directly for PCR, quantitative PCR, southern Blot, test of virus DNA and so on.
Specifications
Features | Specifications |
Main Functions | Isolation total DNA from 50mg simple plant using 96 well bind plate |
Applications | PCR, SSR, AFLP, RAPD and southern blot, etc. |
Purification method | 96 well plate |
Purification technology | Silica technology |
Process method | Manual (centrifugation or vacuum) |
Sample type | Economic plant samples |
Sample amount | Fresh / frozen plant samples: ≤50mgDried plant / seed samples: ≤15mg |
Elution volume | ≥75μl |
Time per run | 30-50 minutes |
Liquid carrying volume per column | 800μl |
Binding yield of column | 100μg |
Plantmaterial is first mechanically disrupted and then lysed by addition of lysis buffer and incubation. RNase A in the lysis buffer digests the RNA in the sample. After lysis, proteins and polysaccharides are salt-precipitated. Binding buffer and ethanol are added to the cleared lysate to promote binding of the DNA to the HiPure membrane. The sample is then applied to a column and then centrifuged. DNA binds to the membrane, while contaminants such as proteins and polysaccharides are efficiently removed by 2 wash steps. Pure DNA is eluted in a small volume of low-salt buffer or water.
Kit Contents
Contents | D316702 | D316703 |
Purification Times | 4 x 96 Preps | 20 x 96 Preps |
RNase Solution | 5 ml | 22 ml |
Elution Buffer | 120 ml | 500 ml |
Buffer SPL | 200 ml | 2 x 500 ml |
Buffer PS | 100 ml | 400 ml |
Buffer GW1 | 220 ml | 5 x 220 ml |
Buffer GW2 | 100 ml | 3 x 100 ml |
1.6ml Collection Plate | 4 | 20 |
HiPure gDNA Plate | 4 | 20 |
2.2ml Collection Plate(DW Plate) | 8 | 40 |
0.8ml Collection Plate(DW Plate) | 4 | 20 |
Sealing Film | 20 | 100 |
Storage and Stability
RNase solution should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect its performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
Name | CAT NO | Fresh / frozen tissue amount | Dried tissue amount | Column type | Elution volume | Yield (muscle) |
HiPure Plant DNA Mini Kit | D3187 | 150mg | 40mg | 1.5ml column | 50 – 100μl | 3-70μg |
HiPure HP Plant DNA Maxi Kit | D3163 | 5g | 1g | 50ml column | 0.7 – 3ml | 75-1250μg |
HiPure SF Plant DNA Kit | D3164 | 100mg | 20mg | 1.5ml column | 50 – 100μl | 3-50μg |
HiPure SF Plant DNA 96 Kit | D3167 | 50mg | 15mg | 96 well plate | 75 – 150μl | 3-30μg |
This product is suitable for rapid extraction of DNA from plant and fungal samples. The kit is based on silica gel column purification technology. The whole extraction process only takes 60-90 minutes. DNA can be used directly for PCR, quantitative PCR, southern Blot, test of virus DNA and so on.
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Tel : 081-875-1869 , 02-328-7179
Email : hej@a3p-scientific.com
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