029070 Nitrate Reduction Reagent

• Set of three Solid Phase Adsorption Toxin Tracking (SPATT) Bags
• Pre activated
• Ready for deployment
• HP20

Description

Solid Phase Adsorption Toxin Tracking (SPATT) is a biomimetic in-situ water monitoring tool that falls under an expanding umbrella of passive samplers. It serves to warn researchers of toxin-producing harmful algal bloom (HAB) developments early on. It has been popularized through its affordability, ease of use, and its ability to capture ephemeral events in marine, brackish, and freshwater environments. Its uptake of contaminants has been shown to be more similar than other sampling methods to that of aquatic species like bivalves, mussels, and clams. It provides an average bioavailable fraction of a toxin over deployment time that can be used to determine an overall toxin risk to organisms. The sampling period typically depends on the bioactivity at a site, ranging from 24 hours to 4 weeks in most cases.

A SPATT passively absorbs and desorbs extracellular compounds over its stretch of time at a sampling site; in an organism, a toxin would go through biochemical detoxification processes. Passive samplers have a higher sensitivity for more compounds and provide improved stability and preservation of these compounds within the resin. SPATT devices capture less commonly detected cyanotoxins (e.g. cylindrospermopsin) at lower concentrations than that of a grab sample (collected at one point in time). Grab samples are limited in scope and sensitivity, and underrepresent toxins like microcystin-LR, which is picked up very reliably through SPATT technology.

Uses HP20 that is widely applicable for many toxins.

Used to capture:

• Cyanotoxin (e.g. microcystin and cylindrospermopsin)
• Saxitoxin & derivatives (GNTXs, C-toxins), and other paralytic shellfish toxins (PSTs)
• Domoic acid (DA)
• Nodularin
• Anatoxin-a
• Brevetoxins (via “red tide”-causing dinoflagellate Karenia brevis)
• Okadaic acid (OA) & derivative Dinophysistoxins (DTXs) and other Diarrheic shellfish poisoning (DSP) toxins
• Yessotoxin (YTX) and Pectenotoxins (PTXs)
• Cyclic imines (CIs), e.g. Spirolides (SPXs), Gymnodimines (GYMs), Pinnatoxins (PnTXs)
• Ciguatoxin (CTX) & precursor Gambiertoxin (GTX), Maitotoxin (MTx), and other ciguatera fish poisoning (CFP) toxins

Set of three Solid Phase Adsorption Toxin Tracking (SPATT) Bags
Pre activated
Ready for deployment
HP20

K-ACETRM

SKU: 700004258

72 assays (manual) / 720 assays (microplate)

The Acetic Acid (Acetate Kinase Manual Format) test kit is suitable for the measurement and analysis of acetic acid in food and beverages.

This rapid and reliable manual acetic acid kit is simple to perform (only two absorbance readings required), and because a true end-point is measured, does not involve complicated calculations like other kits. This product is very stable both during storage and use (> 2 years), has extended linearity (compared to ACS based kits), contains PVP to prevent tannin inhibition, and is performed at a relatively low pH (7.4), thus minimising ester hydrolysis related interference. This method is suitable for the measurement of acetic acid/acetate in foods, beverages and other materials.

Note for Content: The number of manual tests per kit can be doubled if all volumes are halved.  This can be readily accommodated using the MegaQuant^TM  Wave Spectrophotometer (D-MQWAVE).

View all of our acetic acid and other organic acid assay kits.

The Acetic Acid (Acetate Kinase Manual Format) test kit is suitable for the measurement and analysis of acetic acid in food and beverages.

This rapid and reliable manual acetic acid kit is simple to perform (only two absorbance readings required), and because a true end-point is measured, does not involve complicated calculations like other kits. This product is very stable both during storage and use (> 2 years), has extended linearity (compared to ACS based kits), contains PVP to prevent tannin inhibition, and is performed at a relatively low pH (7.4), thus minimising ester hydrolysis related interference. This method is suitable for the measurement of acetic acid/acetate in foods, beverages and other materials.

Introduction

Usages:
For the growth of pathogenic and non-pathogenic microorganisms.

Principle:
Polypeptone provide carbon and nitrogen sources, vitamins and growth factors; Neisseria starch can promote the growth characteristics and enhanced hemolytic streptococci; sodium chloride to maintain osmotic balance; agar as culture medium coagulant.

Formulation (per liter):
Peptone: 10g
Beef extract powder :10g
Sodium chloride: 5g
Agar :15g
Final pH 7.4 ± 0.2

How to use:
1. Suspend 40g of the product, adding 1 L of distilled or deionized water, ,heated to boiling stirring until completely dissolved, dispensing into flask, 121 autoclaved 15min.
2.After the medium is cooled to about 50 , sterile procedures, basal medium was added per 100mL defibrinated sheep or rabbit blood 5-10mL, shake pour plate; also dispensing sterile tubes set into the slope. Spare.
3. take the bacteria to be tested fresh pure cultures inoculated crossed or coated on the plate, set the temperature of the incubator training requirements were cultured for a predetermined time.
4. Observe the results.

Quality control:
The following were inoculated after 36 ± 1 for 24h , the results are as follows:
bacteria name bacteria No. growth status colony characteristics
Escherichia coli ATCC25922 not good hemolysis

Staphylococcus aureus, ATCC6538 good β-hemolytic

Beta-hemolytic streptococcus CMCC (B) 32210 good β-hemolytic

Streptococcus pneumoniae CMCC (B) 31001 good α-hemolytic

Storage: Store in a dark, cool and dry place, tighten the cap immediately after use. Storage period of three years.

500g