
Introduction
029110 Sterile Liquid Paraffin
Usage:For covering a liquid medium for anaerobic culture.
Specification:10ml.
10ml
029110 Sterile Liquid Paraffin
Usage:For covering a liquid medium for anaerobic culture.
Specification:10ml.
RNA into DNA and PCR in one step? Then, this enzyme will simplify PCR analysis from RNA templates reducing labor and time. RT-KTQ2 was evolved from the thermostable KlenTaq DNA polymerase with no significant reverse transcriptase activity. Four mutations ensure that the variant is reverse transcriptase active and even PCR active, while maintaining the thermostability. This allows to perform reactions at high temperatures minimizing problems encountered with strong secondary structures in RNA that melt at elevated temperatures. For further information refer to the original publication.
Available upon request and for R&D use only – Contact Us
RT-KTQ2 DNA polymerase is supplied as a 5 µM solution containing glycerol and is supplied together with 10x reaction buffer.
The enzyme can also be used for real-time cycling, when adding a suitable dye.
RNA into DNA and PCR in one step? Then, this enzyme will simplify PCR analysis from RNA templates reducing labor and time. RT-KTQ2 was evolved from the thermostable KlenTaq DNA polymerase with no significant reverse transcriptase activity. Four mutations ensure that the variant is reverse transcriptase active and even PCR active, while maintaining the thermostability. This allows to perform reactions at high temperatures minimizing problems encountered with strong secondary structures in RNA that melt at elevated temperatures. For further information refer to the original publication.
RAA uses a novel RNA substrate tagged with a fluorescent reporter molecule (fluor) on one end and a quencher on the other. In the absence of RNases, the physical proximity of the quencher dampens fluorescence from the fluor to extremely low levels. When RNases are present, however, the RNA substrate is cleaved, and the fluor and quencher are spatially separated in solution. This causes the fluor to emit a bright green signal when excited by light of the appropriate wavelength. Fluorescence can be readily detected with a fluorometer. Since the fluorescence of the RAA Substrate increases over time when RNase activity is present, results monitored with a fluorometer can be evaluated kinetically. The sequence of the RAA Substrate has been carefully optimized to detect several RNases, including RNase A, RNase T1, RNase I, micrococcal nuclease, S1 nuclease, mung bean nuclease, and Benzonase.
RNase activity in a convenient and sensitive fluorimetric assay that delivers results in real time. Great for Quality Testing for RNase contamination of materials and supplies.
Usage | For cultivating and enriching nofastidious bacteria ,and for evaluating the efficacy of disinfection. |
Formula (per liter) | Peptone—————10.0g Beef Extract———–3.0g Sodium Chloride—–5.0g Final pH———–7.4 ± 0.2 |
How to use | 1.Suspend 18g in 1L of distilled water , stirring heated to boiling to completely dissolve ,autoclave at 121ºC for 15 minutes. 2.Diluted and treated samples. |
Storage | Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years. |
500g
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