
Introduction
029110 Sterile Liquid Paraffin
Usage:For covering a liquid medium for anaerobic culture.
Specification:10ml.
10ml
029110 Sterile Liquid Paraffin
Usage:For covering a liquid medium for anaerobic culture.
Specification:10ml.
UV Cleavable Biotin-PEG2-alkyne contains a UV cleavable fragemnt, the reagent is reactive with azide containing molecule via click chemistry. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
UV Cleavable Biotin-PEG2-alkyne contains a UV cleavable fragemnt, the reagent is reactive with azide containing molecule via click chemistry. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
HiPure Insect DNA Kits provides a simple and rapid solution for total DNA extraction of insect tissue samples. This kit is based on silica gel column purification technology without toxic phenol chloroform extraction and time-consuming alcohol precipitation. The whole extraction process only takes 30 minutes. HiPure Insect DNA Kit can process tissue samples less than 10mg at a time. Hipure Insect DNA 96 kit can process 96 insect tissue samples at a high throughput. The obtained DNA can be directly used in PCR, Southern blot, viral DNA detection and other experiments.
Specifications
Features | Specifications |
Main Functions | Isolation total DNA from insect tissue |
Applications | PCR, southern bolt and virus detection, etc |
Purification method | 96 well DNA plate |
Purification technology | Silica technology |
Process method | Manual (centrifugation or vacuum) |
Sample type | Insect tissue samples |
Sample amount | |
Elution volume | |
Time per run | |
Liquid carrying volume per column | |
Binding yield of column |
Principles
This product is based on silica column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
Contents | D313901 | D313902 |
Purification Times | 1 x 96 | 4 x 96 |
HiPure DNA Plate | 1 | 4 |
2.2 ml Collection Plate | 1 | 4 |
1.6 ml Collection Plate | 1 | 4 |
0.5ml Collection Plate | 1 | 4 |
Seal Film | 8 | 32 |
Buffer ITL | 30 ml | 120 ml |
Buffer IL | 30 ml | 125 ml |
Buffer GW1 | 44 ml | 2 x 110 ml |
Buffer GW2 | 50 ml | 3 x 50 ml |
Proteinase K | 50 mg | 200 mg |
Protease Dissolve Buffer | 6 ml | 15 ml |
Buffer AE | 20 ml | 60 ml |
Proteinase K, RNase A should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2–8°C, but in thiscase buffers should be redissolved before use. Make sure that all buffers areat room temperature when used.
HiPure Insect DNA Kits provides a simple and rapid solution for total DNA extraction of insect tissue samples. This kit is based on silica gel column purification technology without toxic phenol chloroform extraction and time-consuming alcohol precipitation. The whole extraction process only takes 30 minutes. HiPure Insect DNA Kit can process tissue samples less than 10mg at a time. Hipure Insect DNA 96 kit can process 96 insect tissue samples at a high throughput. The obtained DNA can be directly used in PCR, Southern blot, viral DNA detection and other experiments.
Malaria is a mosquito-borne infectious disease and considered one of the leading causes of death in the world. Malaria is a fatal tropical disease that is caused by a parasite knows as Plasmodium. It is usually spread through the bite of an infected female mosquito. 300-500 million new infected cases are estimated every year with 1.5-2.7 million deaths worldwide. Most of the malaria-related deaths occur in sub-Saharan Africa. . Although blood is the sample most frequently used to make a diagnosis, both saliva and urine have been investigated as alternative, less invasive specimens
Malaria TaqMan PCR Kit, 100 reactions
Malaria TaqMan PCR Probe/Primer Set and Controls, 100 reactions
For research use only and NOT intended for in vitro diagnostics.
Figure 1 / 3
Click for expanded view
Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
Component | Cat. TM34850 (100 preps) | Cat. TM34810 (100 preps) |
---|---|---|
MDx TaqMan 2X PCR Master Mix | 2 x 700 μL | – |
Malaria Primer & Probe Mix | 280 μL | 280 μL |
Malaria Positive Control | 150 μL | 150 μL |
Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
Product Insert | 1 | 1 |
83, On-nut 88/2 Prawet Sub-district, Prawet District, Bangkok, 10250, Thailand
Tel : 081-875-1869 , 02-328-7179
Email : hej@a3p-scientific.com
Copyright © 2024 A3P Scientific Co., Ltd. All rights reserved. Web by Mountain Studio
Privacy Policy | Terms of Use | Site Map