10 Layer Cell Factory One Wide Mouth And One Narrow Mouth

Huayi Cell Factory is a robust and easy-to-use cell

culture platform for applications in the production of human and animal vaccines, therapeutic proteins, cell therapy, and gene therapy.

To address the needs of your workflow, We have three kinds of mouth, which is available on most cell culture products to ensure consistent performance from lot to lot and from format to format.

Specifications: 1 layer, 2 layers, 5 layers, 10 layers and 40 layers.

Detail

10 Layer Cell Factory One Wide Mouth And One Narrow Mouth

Huayi Cell Factory is a robust and easy-to-use cell

culture platform for applications in the production of human and animal vaccines, therapeutic proteins, cell therapy, and gene therapy.

To address the needs of your workflow, We have three kinds of mouth, which is available on most cell culture products to ensure consistent performance from lot to lot and from format to format.

Specifications: 1 layer, 2 layers, 5 layers, 10 layers and 40 layers.

PRODUCT FEATURES

The product is made of medical grade USP CLASS VI polymer polystyrene
The product is made under a 100,00- class dust-free manufacturing site
Surface TC treatment ensures efficient cell attachment
The product assembled with ultrasonic welded technology
Versatile port design facilitates both pouring and aseptic filling techniques
Gamma radiation sterilization
the cell culture surface area

of one 10-layer Cell Factory unit is equivalent to the area

of 36 T-175 flasks

Other Products

16S V1-V2 Library Preparation Kit for Illumina

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Overview

Protocol optimized for DNA isolated from a diversity of samples including stool, soil, water, saliva, plant, urine, skin, and more
Simple and quick workflow: library could be prepared in less than 5 hours
Component of Norgen’s metagenomics workflow
A single NGS run can be prepared with up to 384 unique dual-index libraries

Sample type purification kit guide

The 16S V1-V2 Library Preparation Kit for Illumina consists of the reagents and components required for library preparation of the 16S V1-V2 amplicon libraries to be used for next-generation sequencing on Illumina platforms. All molecular reagents including primers, enzyme mixes, indexes, and buffers are provided. Instructions for PCR clean up with the AMPure XP Magnetic Beads (supplied by customer) are also included for rapid purification of nucleic acid products generated at two steps of the workflow. The library prep workflow could be used for purified DNA inputs from different sources including stool, soil, water, saliva, plant, urine, skin swab, vaginal swab, cheek swab, nasal swab, plasma/serum, tongue swab, gum swab, and others.

The 16S V1-V2 Library Preparation Kit for Illumina has a streamlined procedure that reduces the handling time such that the library prep procedure can be completed in approximately 4 hours (see diagram below). Input DNA is first subjected to targeted PCR to amplify the V1-V2 region of the DNA encoding 16S rRNA. The post-PCR reaction is then cleaned up using AMPure XP beads. Dual index primers are then added using a limited-cycle PCR. The indexed amplicons flanked by 5′ and 3′ barcoded adaptors are then cleaned using AMPure XP beads. The libraries are then ready for quantification, pooling and sequencing. 16S Library Prep

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Supporting Data

Figure 1 / 3

Figure 1: 16S V1-V2 PCR1 Amplification

Figure 1: 16S V1-V2 PCR1 Amplification

Figure 2: 16S V1-V2 Indexed Libraries

Figure 3: BioAnalyzer Trace of the 16S V1-V2 Indexed Library

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Minimum amount of starting material:	2.5 µL of DNA (5 ng/µL)
Time to complete library preparation:	4 hours

Storage Conditions and Product Stability
Norgen’s 16S V1-V2 Library Prep Kit for Illumina is shipped as one kit box (for the 24 prep kit) or two sub-component kits (for the 96 prep kit). All kits should be stored at -20°C upon arrival.

All kit components should remain stable for at least 1 year when stored at the specified storage conditions.

Step	Component	Cat. 70100 (24 preps)	Cat. 70110, 70120, 70130, 70140 (96 preps)
Amplicon PCR (PCR 1)	MGX Master Mix	330 µL	1,320 µL
Amplicon PCR (PCR 1)	16S V1-V2 Primer Mix	70 µL	280 µL
Index PCR (PCR 2)	Indexing Master Mix	660 µL	2 x 1,320 µL
	N7xx Index Primer	50 µL	50 µL
	S5xx Index Primer	70 µL	70 µL
PCR Clean-Up	Resuspension Buffer	2 x 1,250 µL	2 x 5,000 µL
	Nuclease-free water	1,250 µL	1 x 6,000 µL

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[QP5210] Q-PAGE™ TGN Precast Gel (Midi, 12 wells, 10%), 10 gels

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Saliva DNA Isolation Kit

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Overview

Fast and easy processing using a rapid spin-column format
Isolate high quality genomic DNA from fresh or preserved saliva samples
Compatible with preserved saliva samples collected using Norgen’s Saliva DNA Collection and Preservation Devices (Cat. RU49000)

This kit provides a fast and simple spin column procedure for isolating genomic DNA from saliva samples collected and preserved using Norgen’s Saliva DNA Collection and Preservation Devices (Cat. RU49000), as well as fresh saliva samples.

Saliva DNA purified using Norgen’s kit is of the highest quality, and is compatible with a number of downstream research applications including PCR, Southern Blot analysis, sequencing and microarray analysis.

Background

Saliva represents an excellent non-invasive alternative to blood collection. Human genomic DNA extracted from buccal epithelial cells and white blood cells found in saliva can be used in various applications in diagnostics. Saliva DNA can be used for the detection of biomarkers to diagnose a disease, follow the diseases progress or monitor the effects of a particular treatment. Saliva DNA can also be used to diagnose particular types of infections. Isolation of DNA from saliva has become an attractive alternative to isolation from blood or tissue due to the fact that sample collection is non-invasive, the samples can be collected by individuals with little training, and no special equipment is required. Norgen’s Saliva DNA Isolation Kit provides a fast and simple procedure for isolating genomic DNA from both preserved saliva samples and fresh saliva samples.

Details

Supporting Data

Figure 1 / 3

Figure 1. High Quality and Yield of DNA from Saliva Samples.

Figure 1. High Quality and Yield of DNA from Saliva Samples.

Figure 2. Real-time PCR Consistency from Saliva Samples.

Figure 3. Illumina MiSeq 16s rRNA metagenomics data from saliva.

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Kit Specifications
Maximum Saliva Input	0.5 mL preserved saliva 0.25 mL fresh saliva
Average Yield from 0.25 mL of Saliva*	3 – 7 μg
Average Purity (OD260/280)	1.7 – 2.1
Time to Complete 10 Purifications	30 minutes

* Average yield will depending on the donor

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 1 year after the date of shipment when stored at room temperature.

Component	Cat. RU45400 (50 preps)
Lysis Buffer F	30 mL
Proteinase K in Storage Buffer	1.2 mL
Binding Buffer B	12 mL
Wash Solution A	18 mL
Elution Buffer B	15 mL
Spin Columns	50
Collection Tubes	50
Elution Tubes (1.7 mL)	50
Product Insert	1

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