100 bp DNA Ladder in Ready-to-load format

Overview

• Ready-to-use
• Quantitative
• Highly Stable
• Precise
• Fifteen discrete fragments ranging from 300 bp to 24000 bp
• Higher intensity reference bands at 5000 bp and 10000 bp

The Norgen UltraRanger 1 kb DNA Ladder is prepared to ensure quality and batch-to-batch consistency. Our UltraRanger contains fifteen discrete fragments ranging from 300 bp to 24000 bp with higher intensity reference bands at 5000 bp and 10000 bp. This ladder is ideal for sizing digested DNA of high molecular weight.

Contents
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).

Details

Supporting Data

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UltraRanger 1kb DNA Ladder (Cat# 12100) – 100 loads

Ladder Properties:
• Fifteen discrete fragments ranging from 300 bp to 24000 bp

• Higher intensity reference bands at 5000 bp and 10000 bp

Fragment	Size (bp)	Mass (ng)
1	24000	30
2	14000	27
3	10000	60
4	8000	44
5	6000	36
6	5000	55
7	4000	49
8	3000	39
9	2500	35
10	2000	30
11	1500	33
12	1000	10
13	700	21
14	500	18
15	300	11

Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.

Storage: 
Stable at room temperature. For longer term storage, -20°C is recommended.

This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.

Propargyl-PEG14-alcohol is a propargyl linker which enables Click Chemistry with azide compounds to yield stable triazole linkage. The PEG spacer enhances the hydrophilicity of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Propargyl-PEG14-alcohol is a propargyl linker which enables Click Chemistry with azide compounds to yield stable triazole linkage. The PEG spacer enhances the hydrophilicity of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.