Introduction

MagPure Seed DNA Kit supplies a simple and rapid extraction of genomic DNA from different plant pieces and seed. This kit is based on superparamagnetic particles purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction only takes 60 minutes. This kit can be used on different automatic extraction machines like KingFisher ML, KingFisher Flex and KingFisher Duo. Purified DNA can be used directly for PCR, quantitative PCR, southern blot, hybridizationand transgenosis detection.

Details

Specifications

Features	Specifications
Main Functions	Isolation total DNA from 50-100 mg easy-grinded plant (tender leaf) and seed
Applications	PCR, transgene detection, fluorescence quantitative PCR, southern blot, SNP site analysis, etc.
Purification technology	Magnetic beads technology
Process method	Manual or automatic
Sample type	Conventional economic plant samples
Sample amount	50-100mg
Elution volume	≥50μl
Time per run	≤60 minutes

Principle

This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.

Advantages

• Safe – require no phenol or chloroform extraction
• Fast – several samples can be extracted in 60 minutes
• High purity – high quality DNA, completely remove inhibitors

Kit Contents

Contents	D635201	D635202	D635203
Purification Times	48 Preps	96 Preps	5 x 96 Preps
MagPure Particles	1.7 ml	4 ml	18 ml
Buffer SOL	40 ml	90 ml	500 ml
Buffer SDS	4 ml	9 ml	50 ml
Buffer MPB	40 ml	70 ml	350 ml
Buffer GW1	26 ml	53 ml	220 ml
Elution Buffer	10 ml	30 ml	120 ml

Storage and Stability

MagPure Particles should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

Experiment Data

MagPure Seed DNA Kit supplies a simple and rapid extraction of genomic DNA from different plant pieces and seed. This kit is based on superparamagnetic particles purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction only takes 60 minutes. This kit can be used on different automatic extraction machines like KingFisher ML, KingFisher Flex and KingFisher Duo. Purified DNA can be used directly for PCR, quantitative PCR, southern blot, hybridizationand transgenosis detection.

Assay Tubes (Qubit Fluorometer)

The Qubit assay tubes are thin-wall polypropylene tubes (500 µl) for use with the Qubit Fluorometer. High-quality of the tubes guarantee the accurate measurement of different types of samples such as DNA (dsDNA, ssDNA), RNA and protein.

Qubit tubes from the same lot should be used for the experiments for optimal result. 500 tubes per package.

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Features

• • High-quality: Accurate measurement of different types of samples
  • Cost cutting: Half price as compared to other vendors

Specifications

Shipping condition	Room temperature
Type	Assay tube (for Qubit fluorometer)
For use with (equipment)	Qubit fluorometer
Storage	Store at room temperature
Package	500 tubes

Qubit is a registered trademark of ThermoFisher Scientific.

The Qubit assay tubes are thin-wall polypropylene tubes (500 µl) for use with the Qubit Fluorometer. High-quality of the tubes guarantee the accurate measurement of different types of samples such as DNA (dsDNA, ssDNA), RNA and protein.

Overview

• Detection kits for Legionella sp.
• Available for TaqMan format for analysis

Norgen’s Legionella sp. TaqMan PCR Kit is designed for the detection of Legionella sp. specific DNA in a real-time PCR based on the use of TaqMan technology. This kit is designed for research use only and not for use in diagnostic procedures. The detection of Legionella sp. specific DNA is based on TaqMan PCR providing a simple, reliable and rapid result for the detection of Legionella sp. infection. Norgen’s Legionella sp. TaqMan PCR Kit includes a PCR control to monitor for PCR inhibition, and to validate the quality of the sample and the detection result. The Legionella sp. TaqMan PCR Kit comprises Master Mix for the target and PCR control detection, Primer & Probe Mix, as well as a positive control and a negative control (nuclease-free water) to confirm the integrity of the kit reagents.

Legionella sp. TaqMan PCR Kit, 100 reactions

• Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
• Specific Primer and Probe mix for the pathogen/virus/viroid of interest
• Primer and Probe mix
• Positive and negative control to confirm the integrity of the kit reagents

Legionella sp. TaqMan PCR Probe/Primer Set and Controls, 100 reactions

• Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
• Nuclease-free water
• Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix

For research use only and NOT intended for in vitro diagnostics.

Details

Supporting Data

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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.

Component	Cat. TM64450 (100 rxns)	Cat. TM64410 (100 rxns)
MDx TaqMan 2X PCR Master Mix	2 x 700 mμL	–
Legionella sp. Primer & Probe Mix	280 μL	3 x 70 μL
Legionella sp. Positive Control	150 μL	3 x 50 μL
Nuclease-Free Water (Negative Control)	1.25 mL	1.25 mL
Product Insert	1	1