100ml Tube

Propargyl-PEG2-amine is a crosslinker that is reactive with NHS esters, carbonyls (carboxylic acid, ketone, aldehyde). The propargyl group can participate in copper catalyzed azide-alkyne Click Chemistry reaction to form a stable triazole linkage. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Propargyl-PEG2-amine is a crosslinker that is reactive with NHS esters, carbonyls (carboxylic acid, ketone, aldehyde). The propargyl group can participate in copper catalyzed azide-alkyne Click Chemistry reaction to form a stable triazole linkage. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Description 

The G-HiFi™ DNA Polymerase is a new genetically modified, recombinant DNA polymerase suitable for GC-rich templates that are difficult to amplify. The fidelity of G-HiFi™ DNA Polymerase is 70 times higher than that of Taq DNA polymerase. The high extension rate of G-HiFi™ DNA Polymerase is achieved by blending the DNA polymerase with an elongation enhancer. The optimized 5X G-HiFi™ Buffer includes special ingredients that suppress non-specific amplification as well as plateau effect produced by conventional PCR. With the optimized 5X G-HiFi™ Buffer, G-HiFi™ DNA Polymerase is capable to amplify most templates, such as longer targets (up to 40 kb from lambda DNA) and that contain GC-rich sequences. 

Features

• 5’→3’ DNA polymerase activity 
• 3’→5’ exonuclease (proofreading) activity
• Suitable for GC-rich templates 
• High reaction rate: 7 seconds/kb 
• High fidelity: 70 times higher than Taq polymerase 
• Generates blunt end amplicons  
• Vast elongation capability (up to 40 kb) 
• Thermo-stable for more than 10 hrs at 95°C 

Storage

[TF3000] G-HiFi™ DNA Polymerase

-20°C for 24 months

The G-HiFi™ DNA Polymerase is a new genetically modified, recombinant DNA polymerase suitable for GC-rich templates that are difficult to amplify. The fidelity of G-HiFi™ DNA Polymerase is 70 times higher than that of Taq DNA polymerase. The high extension rate of G-HiFi™ DNA Polymerase is achieved by blending the DNA polymerase with an elongation enhancer. The optimized 5X G-HiFi™ Buffer includes special ingredients that suppress non-specific amplification as well as plateau effect produced by conventional PCR. With the optimized 5X G-HiFi™ Buffer, G-HiFi™ DNA Polymerase is capable to amplify most templates, such as longer targets (up to 40 kb from lambda DNA) and that contain GC-rich sequences.