12x100ml Angle Rotor 

Introduction

Usages:
For cultivating of bacteria.And sterility test of drugs and biological products.

Principle:
Tryptone, peptone and yeast extract multivalent powder provides a nitrogen source, vitamins, and growth factors; sodium chloride to maintain osmotic balance; glucose carbon source; dipotassium hydrogen phosphate as a buffering agent.

Formulation（per liter）:
Pancreatic Digest of Casein            17g
Papaic Digest of Soybean              3g
Sodium chloride                     5g
Dipotassium hydrogen phosphate       2.5g
Glucose Monohydrate                2.5g
Final pH 7.3±0.2  

How to use：
1.Suspend 30g in 1L of distilled water , stirring heated to boiling to completely dissolve ,autoclave at 121℃ for 15 minutes.
2.Diluted and treated samples.

Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.

Specifications: 250g/bottle

250g

Propargyl-PEG7-acid is a reagent with a propargyl group with a carboxylic acid. The carboxylic acid reacts with primary amines under the activation of EDC or HATU. The propargyl group can participate in azide-alkyne Click Chemistry reaction to form triazole linkage, copper is required as a catalyst. The PEG spacer increases the hydrophilicity of the molecule. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Propargyl-PEG7-acid is a reagent with a propargyl group with a carboxylic acid. The carboxylic acid reacts with primary amines under the activation of EDC or HATU. The propargyl group can participate in azide-alkyne Click Chemistry reaction to form triazole linkage, copper is required as a catalyst. The PEG spacer increases the hydrophilicity of the molecule. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Overview

• Removes primer dimers
• Simultaneously clean-up and normalize PCR products 
• Fast (less than 20 minutes), high-throughput and easy processing using centrifugation
• Sufficient elution volume (100 µL) for repeat or future assays 
• Non-magnetic bead purification 

Norgen’s NGS Normalization 96-Well Kit allows for high-throughput NGS library PCR amplicon clean-up and normalization of NGS library PCR product concentration. The clean-up removes all PCR by-products including primers, dimers, enzymes and unincorporated nucleotides during the library prep PCR step. Purification is based on 96-well column chromatography using Norgen’s proprietary resin as the separation matrix. The amount of Norgen’s matrix in each 96 well is optimized to have a limited binding capacity, thus each well can elute an equal concentration of PCR product. The process involves first adding 3 volumes of Buffer SK to the PCR product, and the mixture is then loaded onto the 96-Well Normalization Plate. The bound PCR amplicon is then washed with the provided Wash Solution A in order to remove any remaining impurities, and the purified PCR amplicon is eluted with Elution Buffer B. The purified and normalized library PCR amplicon can then be used in NGS workflows and other sequencing applications.

Details

Supporting Data

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Storage Conditions
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Component	Cat. 61900 (192 preps)
Buffer SK	30 mL
Wash Solution A	2 x 38 mL
Elution Buffer B	30 mL
96-Well Normalization Plate	2
96-Well Collection Plate	2
96-Well Elution Plate	2
Adhesive Tape	1
Product Insert	1