This kit enables the rapid purification of amplified DNA products from PCR mixes. It is able to effectively remove PCR by-products including primers, dimers, enzymes, unincorporated nucleotides and mineral oil from the desired PCR product. The purified PCR products are fully compatible with restriction enzyme digestion, ligation into vectors, labeling, sequencing and more. This kit can also be used as an alternative to organic extraction and ethanol precipitation to clean up various enzymatic reactions.
The kit is also available in a 96-well format for high-throughput PCR purification. Purification with the 96-well plate can be performed using either a vacuum manifold or centrifugation.
Figure 1 / 2
Click for expanded view
| Kit Specifications – Spin Column | |
| Column Binding Capacity | 10 μg |
| Size of DNA Purified | 100 – 15,000 bp |
| Average Recovery | > 90% |
| Average Primer Removal | > 90% |
| Minimum Elution Volume | 30 μL |
| Time to Complete 10 Purifications | 15 minutes |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
| Component | Cat. 14400 (50 preps) | Cat. 45700 (250 preps) | Cat. 24800 (192 preps) |
|---|---|---|---|
| Binding Buffer C | 30 mL | 5 x 30 mL | 3 x 30 mL |
| Wash Solution A | 12 mL | 2 x 20 mL | 2 x 38 mL |
| Elution Buffer B | 8 mL | 2 x 30 mL | 2 x 15 mL |
| Spin Columns | 50 | 250 | – |
| Collection Tubes | 50 | 250 | – |
| 96-Well Plate | – | – | 2 |
| Adhesive Tape | – | – | 4 |
| 96-Well Collection Plate | – | – | 2 |
| Elution Tubes (1.7 mL) | 50 | 250 | – |
| 96-Well Elution Plate | – | – | 2 |
| Product Insert | 1 | 1 | 2 |
This kit is designed for the rapid spin column preparation of genomic DNA from 2 x 109 viable bacterial cells (between 0.5 and 1.0 mL of culture). This kit can be used for both Gram-negative and Gram-positive bacteria including Escherichia coli and Bacillus cereus. Purified genomic DNA is of an excellent quality and yield, and is fully compatible with restriction enzyme digestions, sequencing, PCR, qPCR and more. Also available in 96-well format for high throughput applications.
Figure 1 / 2
Click for expanded view
| Kit Specifications (96-well) | |
| Maximum Input | 2 x 109 bacterial cells |
| Well Binding Capacity | 25 µg |
| Average Yield* | Up to 20 µg* |
| Time to Complete 10 Purifications | 90 minutes |
* Yield will vary depending on the type of sample processed
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 1 year after the date of shipment when stored at room temperature.
| Component | Cat. 17900 (50 preps) | Cat. 17950 (192 preps) |
|---|---|---|
| Resuspension Solution A | 20 mL | 60 mL |
| Lysis Buffer P | 18 mL | 60 mL |
| Solution BX | 28 mL | 110 mL |
| Wash Solution A | 18 mL | 2 x 38 mL |
| Elution Buffer B | 30 mL | 2 x 30 mL |
| Proteinase K | 12 mg | 50 mg |
| Spin Columns | 50 | – |
| 96-Well Plate | – | 2 |
| Adhesive Tape | – | 4 |
| Collection Plate | – | 2 |
| Collection Tubes | 50 | – |
| Elution Tubes (1.7 mL) | 50 | – |
| Elution Plate | – | 2 |
| Product Insert | 1 | 1 |
For scaling up and fully automating PCR setup and thermocycling.
With the Flex PCR Workstation, you can automate your PCR setup and thermocycling at the scale you need to increase efficiency, reduce errors, and save hands-on time. Configure your workstation with our on-deck Thermocycler for end-to-end automation, or with no Thermocycler for automated PCR setup with off-deck thermocycling.
Optional add-ons can be purchased at a 10% discount when ordered with the Flex PCR Workstation*
For scaling up and fully automating PCR setup and thermocycling.
With the Flex PCR Workstation, you can automate your PCR setup and thermocycling at the scale you need to increase efficiency, reduce errors, and save hands-on time. Configure your workstation with our on-deck Thermocycler for end-to-end automation, or with no Thermocycler for automated PCR setup with off-deck thermocycling.
Optional add-ons can be purchased at a 10% discount when ordered with the Flex PCR Workstation*
83, On-nut 88/2 Prawet Sub-district, Prawet District, Bangkok, 10250, Thailand
Tel : 081-875-1869 , 02-328-7179
Email : hej@a3p-scientific.com
Copyright © 2024 A3P Scientific Co., Ltd. All rights reserved. Web by Mountain Studio
Privacy Policy | Terms of Use | Site Map