Discover Huankai’s GC Agar Base, designed for the selective cultivation and isolation of Neisseria gonorrhoeae and Neisseria meningitidis, ensuring reliable and accurate microbial detection.
Discover Huankai’s GC Agar Base, designed for the selective cultivation and isolation of Neisseria gonorrhoeae and Neisseria meningitidis, ensuring reliable and accurate microbial detection.
The 50 bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. This Ladder contains ten discrete fragments ranging from 50 bp to 500 bp with a higher intensity reference band at 250 bp. This Ladder is ideal for quick sizing of PCR products
Contents
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).
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50 bp DNA Ladder (Cat# 13525) – 100 loads
Ladder Properties:
• Ten discrete bands, ranging from 50 bp to 500 bp
• Higher intensity band at 250 bp for easy reference
| Fragment | Size (bp) | Mass (ng) |
| 1 | 500 | 79 |
| 2 | 450 | 67 |
| 3 | 400 | 59 |
| 4 | 350 | 55 |
| 5 | 300 | 49 |
| 6 | 250 | 76 |
| 7 | 200 | 33 |
| 8 | 150 | 22 |
| 9 | 100 | 31 |
| 10 | 50 | 29 |
Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Storage:
Stable at room temperature. For longer term storage, -20°C is recommended.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit.
Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
Exceptional value for money
Rapid detection of all clinically relevant subtypes
Positive copy number standard curve for quantification
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target
Accurate controls to confirm findings