Giardiasis is a disease of the small bowel caused by the protozoan parasite Giardia intestinalis (syn.duodenalis or lamblia). Giardia is one of the most common intestinal parasites in the world, and occurs at very high prevalence rates in places with poor water sanitation. Individuals become infected through ingesting or coming into contact with contaminated water, food or soil. It can also be spread through the faecal-oral route due to poor hygiene practices, which makes it common in day-care centers. G. intestinalis lives inside the intestines of infected humans or other animals including cats, dogs, birds, cows, beaver and deer. Symptoms of infection include diarrhea, malaise, excessive gas, bloating, nausea, diminished interest in food, possible vomiting and weight loss.
Giardia intestinalis TaqMan RT-PCR Kit, 24 reactions
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Storage Conditions
The Giardia intestinalis TaqMan RT-PCR Kit Dx is shipped on dry ice. The components of the kit should be frozen upon arrival. If one or more of the components is not frozen when the kit is received, or if any of the components have been compromised during shipment, please contact Norgen Biotek for assistance. All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 3 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
| Component | Cat. DxTM43800 (24 rxns) |
|---|---|
| MDx TaqMan 2X RT-PCR Master Mix Dx | 550 µL |
| Giardia intestinalis Primer & Probe Mix Dx | 2 x 70 µL |
| Giardia intestinalis Positive Control Dx – 200,000 copies/μL | 50 µL |
| Nuclease-Free Water | 2 x 1.25 mL |
| Product Insert | 1 |
| Clone | IHC002 |
| Source | Mouse Monoclonal |
| Positive Control | Breast Carcinoma |
| Dilution Range | 1:200 |
The Her2/Neu (c-erbB-2) proto-oncogene is a transmembrane receptor tyrosine kinase that is clinically indicated in a number of carcinomas. Overexpression of the c-erbB-2 protein has been associated with ductal breast cancer, as well as pulmonary and gastric adenocarcinomas. A correlation between Her2 and p53 has also been documented, as overexpression of both proteins has been associated with early invasion and metastasis in bladder cancer.
Propargyl-PEG2-acid is a linker consisting of propargyl and carboxylic acid functional groups. In the presence of activators (e.g. EDC, or HATU), the carboxylic acid reacts with primary amine to form a stable amide bond. The propargyl group reacts with azide group in biomolecules to yield a stable triazole linkage. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Propargyl-PEG2-acid is a linker consisting of propargyl and carboxylic acid functional groups. In the presence of activators (e.g. EDC, or HATU), the carboxylic acid reacts with primary amine to form a stable amide bond. The propargyl group reacts with azide group in biomolecules to yield a stable triazole linkage. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.