500g/bottle;10KG/bag
Usages:
Beef extract powder is a purified extract of beef .Used with meat and casein peptones as a source of nutrients in culture media .
Technical specification:
Total nitrogen(TN)—————————≥12.0%
Amino nitrogen(AN) ————————≤2.7%
Chlorides————————————–＜6%
Moisture—————————————≤5.0%
PH———————————————–6-7
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light.
Specifications: 500g/bottle; 10kg /bag
This product provides fast and easy methods for purification of total DNA for reliable PCR and southern blotting. Total DNA (e.g., genomic, plasmid) can be purified from bacterial cultures.
Specifications
| Features | Specifications |
| Main Functions | Isolation total DNA from bacterial culture |
| Applications | PCR, southern blot and enzyme digestion, etc. |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Sample type | Gram negative or positive bacteria |
| Sample amount | Bacterial culture: 0.5-1.5ml |
| Elution volume | ≥60μl |
| Time per run | ≤60 minutes |
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Lysozyme. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.
Kit Contents
| Contents | D513101 | D513102 | D513103 |
| Purification Times | 48 Preps | 96 Preps | 480 Preps |
| MagPure Particles | 1.2 ml | 2.5 ml | 11 ml |
| Lysozyme | 60 mg | 120 mg | 600 mg |
| Proteinase K | 24 mg | 50 mg | 220 mg |
| Protease Dissolve Buffer | 3 ml | 6 ml | 30 ml |
| Buffer SDS | 1.5 ml | 3 ml | 15 ml |
| Buffer MLA | 30 ml | 60 ml | 300 ml |
| Buffer GW1* | 13 ml | 44 ml | 110 ml |
| Buffer TE | 30 ml | 40 ml | 200 ml |
Storage and Stability
MagPure Particles, Lysozyme and Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
This product provides fast and easy methods for purification of total DNA for reliable PCR and southern blotting. Total DNA (e.g., genomic, plasmid) can be purified from bacterial cultures.
T7 RNA Polymerase catalyzes the formation of RNA from a DNA template in the 5’-3’ direction and is commonly used in in vitro transcription (IVT) applications. The enzyme is T7 promoter specific, requires Mg2+ as a cofactor and can use modified nucleotides for the synthesis. The T7 RNA Polymerase requires a double-stranded DNA template and can produce full-length RNA transcripts.
Key Features
Suggested Applications
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Quality Control
ArcticZymes is dedicated to the quality of our products. T7 RNA Polymerase is manufactured at our ISO 13485 certified facility in Norway.
T7 RNA Polymerase catalyzes the formation of RNA from a DNA template in the 5’-3’ direction and is commonly used in in vitro transcription (IVT) applications. The enzyme is T7 promoter specific, requires Mg2+ as a cofactor and can use modified nucleotides for the synthesis. The T7 RNA Polymerase requires a double-stranded DNA template and can produce full-length RNA transcripts.
