This kit provides a rapid spin column method for the purification of total DNA from a broad spectrum of bacteriophages propagated in bacteria grown in liquid cultures. The DNA is isolated without the use of phenol, chloroform or cesium chloride banding procedures. The spin-column based procedure is rapid and can be completed in less than 45 minutes. The kit is highly efficient for processing small volumes of phage supernatant (500 µL – 1 mL) and with the optional DNase and Proteinase K treatments phage DNA yields are maximized while host DNA contamination is minimized. Purified total phage DNA is of the highest integrity, and can be used in a number of downstream applications including PCR, qPCR, Restriction Fragment Length Polymorphism (RFLP), sequencing, cloning, Southern Blot and more.
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| Kit Specifications | |
| Column Binding Capacity | 50 µg |
| Maximum Column Loading Volume | 650 µL |
| Size of DNA Purified | All sizes |
| Maximum Amount of Starting Material | 1 x 1010 pfu/mL enriched phages |
| Average Yield* | 3-15 µg DNA from 106-1010 pfu/mL of enriched phages |
| Time to Complete 10 Purifications | 45 minutes |
* Average yields will vary depending upon a number conditions used and developmental stage.
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
| Component | Cat. 46800 (50 preps) | Cat. 46850 (100 preps) |
|---|---|---|
| Lysis Buffer B | 40 mL | 2 x 40 mL |
| Wash Solution A | 38 mL | 2 x 38 mL |
| Elution Buffer B | 8 mL | 2 x 8 mL |
| Spin Columns | 50 | 100 |
| Collection Tubes | 50 | 100 |
| Elution Tubes (1.7 mL) | 50 | 100 |
| Product Insert | 1 | 1 |
Norgen’s Fecal DNA Collection & Preservation Mini Tubes are designed for the rapid preservation of DNA from fresh fecal specimens. These tubes contain Norgen’s Fecal DNA Preservative in a liquid format. Moreover, each collection tube is preloaded with beads for effective sample homogenization in any commercially available bead beater equipment (e.g. MP Biomedicals’ FastPrep®-24). The user simply collects fecal samples into the tubes and mixes by shaking or vortexing until the feces is mixed well with preservative. The preservative prevents the growth of Gram-negative and Gram-positive bacteria and fungi, and also inactivates viruses allowing the resulting non-infectious samples to be handled and shipped safely. In addition, the preservative eliminates the need to immediately process or freeze samples and allows the samples to be shipped to centralized testing facilities at ambient temperatures. The components of the preservative allow the DNA in the samples to be preserved at room temperature for over 2 years.
This kit is highly suited to collection and preservation of fecal material from mouse, hamster, chinchilla, rabbit and more for microbiome studies and other applications.
DNA Isolation from Preservative
Fecal samples collected and preserved in Norgen’s Fecal DNA Collection and Preservation Mini Tubes are compatible with most fecal DNA isolation methods. Samples stored in the tubes have been used successfully with all of Norgen Biotek’s stool-based DNA isolation kits (please refer to Related Products table) and reagents.
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| Kit Specifications | |
| Fecal Input | Maximum 30 mg (approximately 3-5 mouse droppings) |
| Stability of Fecal DNA at Room Temperature | 2 years |
Storage Conditions and Product Stability
All tubes should be kept tightly sealed and stored at room temperature (15 – 25°C) for up to 2 years without any reduction in performance.
| Kit Components | Cat. 27650 |
|---|---|
| Fecal DNA Collection & Preservation Mini Tubes (containing liquid preservative and beads) | 12 |
| Product Insert | 1 |
