DBCO-Sulfo-Link-biotin is an anqueous soluble biotin reagent for introducing a biotin moiety to proteins and cell surfaces containing azide moiety through copper-free Click Chemistry. This biotin reagent is membrane-impermeable due to its negatively charged, water soluble sulfonate group. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
DBCO-Sulfo-Link-biotin is an anqueous soluble biotin reagent for introducing a biotin moiety to proteins and cell surfaces containing azide moiety through copper-free Click Chemistry. This biotin reagent is membrane-impermeable due to its negatively charged, water soluble sulfonate group. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
The Norgen water purification system has been designed in conjunction with experts from Millipore Sigma. The Ultrapure NFW is purified in a two-stage process. The first stage, to produce type II water, includes pre-treatment with activated charcoal and polyphosphate, this water then undergoes reverse osmosis, electro-deionization and ion exchange to remove contaminants as well as UV treatment for disinfection. The Pure water then flows to a second system in a clean room with an additional 4 layers of purification. After passing through an additional ion exchange column, the water is then subjected to Ultrafiltration to remove particulates, bacteria, & ionic contaminants down to trace level, the water is then treated with UV light (182 nm wavelength) to break down organic matter. Lastly a second Ultrafilter for the production of pyrogen-, nuclease- and bacteria-free water at 18.2 MΩ. This water is of utmost purity and suitable for direct use in molecular biology applications such as qPCR and NGS, as well as cell culture and other applications.
| Product Specifications | |
| pH | n/a (highly pure water does not contain enough ions for an exact pH determination.) |
| Recommended Storage | Room Temperature |
| Grade | Ultrapure (ASTM Type I) |
| Biological Activity | DNase-, RNase-, Protease-, Endotoxin- Free |
| TOCs | < 50 ppb |
| Resistivity | > 18 MΩ-cm |
| Treatment | Not DEPC-Treated |
| Quantity | 100 mL, 500 mL, 1000 mL |
| Purification Methods | Activated charcoal, polyphosphate, reverse osmosis, electro-deionization, ion exchange, UV, ultrafiltration |
| Shipping Conditions | Room Temperature |
Applications
For use in any molecular biology application
Storage Conditions
Norgen’s Nuclease-Free Water should be stored at room temperature. Storage at +4°C or -20°C is optional.
Precautions and Disclaimers
This product is designed for research purposes only. It is not intended for human or diagnostic use.
Norovirus is a single-stranded, non-enveloped RNA virus belonging to the Caliciviridae family. Norovirus is considered the major causative agent of non-bacterial gastroenteritis in the world. The main channel of transmittance is via contaminated food or water, as well as human-to-human contact. Most Noroviruses infecting humans belong to genogroup I and II (particularly genotype 4). Norovirus is very stable in the environment and is resistant to some surface disinfectants such as alcohols and detergents. Moreover, it is very difficult to culture Norovirus in vitro, making its identification by standard microbiological assays challenging.
NoV TaqMan RT-PCR Kit, 100 reactions
NoV TaqMan RT-PCR Probe/Primer Set and Controls, 100 reactions
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Storage Conditions and Product Stability
All kit components can be stored for 1 year after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
| Component | Cat. TM41450 (100 preps) | Cat. TM41410 (100 preps) |
|---|---|---|
| MDx TaqMan 2X PCR Master Mix | 2 x 700 μL | – |
| NoV Primer & Probe Mix | 280 μL | 280 μL |
| NoV Positive Control | 150 μL | 150 μL |
| Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
| Product Insert | 1 | 1 |