12×30ml Angle Rotor 

Description 

The ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) is a ready-to-use reagent with all the essential components for quantitative real-time PCR (qPCR) except primers and templates. The master mix contains a highly stable hot-start Taq polymerase in an optimized buffer with dsDNA specific SYBR green fluorescent dye. Consequently, the ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) features high stability during storage, even at 37°C for weeks. In addition to high sensitivity and signal intensity, the ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) performs a low background/ high specificity qPCR results, as well as a better compatibility with fast PCR program. With inert smart blue contrast dye, the ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) is ready-to-use and greatly reduces pipetting errors, while largely improving the reproducibility of the process. The ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) also includes ROX reference dye for normalizing the fluorescent reporter signal in real-time quantitative PCR. This master mix allows sensitive, precise amplification, real-time tracking of the amplification process, and simultaneous quantification for targeted DNA molecules.

Features

• High Stability
• Fast Hot Start
• High Sensitivity
• Low Background
• Suitable for Fast Program 
• Smart Blue Contrast Dye 
• With ROX Reference Dye

Storage

Protect from light.
Aliquot to avoid multiple freeze-thaw cycles. 
-20°C for 12 months

The ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) is a ready-to-use reagent with all the essential components for quantitative real-time PCR (qPCR) except primers and templates. The master mix contains a highly stable hot-start Taq polymerase in an optimized buffer with dsDNA specific SYBR green fluorescent dye. Consequently, the ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) features high stability during storage, even at 37°C for weeks. In addition to high sensitivity and signal intensity, the ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) performs a low background/ high specificity qPCR results, as well as a better compatibility with fast PCR program. With inert smart blue contrast dye, the ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) is ready-to-use and greatly reduces pipetting errors, while largely improving the reproducibility of the process. The ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) also includes ROX reference dye for normalizing the fluorescent reporter signal in real-time quantitative PCR. This master mix allows sensitive, precise amplification, real-time tracking of the amplification process, and simultaneous quantification for targeted DNA molecules.

 K-PECID

SKU: 700004325

500 assays per kit

Content:	500 assays per kit
Shipping Temperature:	Ambient
Storage Temperature:	Short term stability: Ambient, Long term stability: See individual component labels
Stability:	> 2 years under recommended storage conditions

Analyte:	Pectin
Assay Format:	Spectrophotometer
Detection Method:	Absorbance
Wavelength (nm):	235
Signal Response:	Increase
Reaction Time (min):	~ 30 min
Application examples:	Food ingredients (e.g. citrus fruit and apple) and other materials.

The Pectin Identification Assay Kit is suitable for the identification of pectin in food ingredients. This kit now employs a new pectate lyase from Aspergillus niger.

See the complete list of our polysaccharide assay kit products.

Advantages

• Very cost effective 
• All reagents stable for > 2 years after preparation 
• Only enzymatic kit available 
• Simple format 
• Standard included

The Pectin Identification Assay Kit is suitable for the identification of pectin in food ingredients. This kit now employs a new pectate lyase from Aspergillus niger.

Overview

• Ensure optimal results for sensitive applications like NGS
• Up to a tenfold increase in microRNA mapping during sequencing runs to reduce costs
• Isolates all sizes of exosomal and extracellular vesicle RNA, including microRNA.
• Bind and elute all RNA irrespective of size or GC content, without bias.
• No phenol extractions.
• No Proteinase K treatment.
• No carrier RNA.
• Concentrate isolated RNA into a flexible elution volume ranging from 50 μL to 100 μL.
• Purify high-quality RNA in 15-20 minutes.
• Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing.
• Purification is based on EXTRAClean spin column chromatography that uses Norgen’s proprietary separation matrix.

The kit is designed to isolate all sizes of RNA, including microRNA. The kit provides a clear advantage over other available kits in that it does not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kit allows the user to elute into a flexible elution volume ranging from 50 μL to 100 μL. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

Details

Supporting Data

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Sample Type	Exosomes purified using Norgen’s Purification Kits
Size of RNA Purified	All sizes, including miRNA and small RNA (< 200 nt)
Elution Volume	50-100 μL
Time to Complete 10 Purifications	35-40 minutes
Average Yields	Variable depending on specimen

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.