MagPure Stool DNA Kit is specially designed for high throughput DNA extraction from stool samples. It can get high purity microbial DNA from stool samples (≤200mg). This kit is based on magnetic beads purification and unique inhibiting factors adsorption technology, no phenol-chloroform extraction or alcohol precipitation. It can adsorb humic acid and other inhibiting factors in the solution efficiently. DNA can be directly used for downstream applications such as PCR, Viral DNA testing, bacterial DNA testing, etc.
Specifications
Features | Specifications |
Main Functions | Isolation total DNA from 100-150mg stool samples |
Applications | PCR, southern blot and enzyme digestion, etc. |
Purification technology | Magnetic beads technology |
Process method | Manual or automatic |
Sample type | Stool |
Sample amount | 100-150 mg |
Elution volume | |
Time per run |
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Lysozyme. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.
Kit Contents
Contents | D6364 |
Purification Times | 400 Preps |
MagPure Particles N | 14 ml |
2ml Bead Tubes | 400 |
PVP-10 | 6 g |
RNase A | 75 mg |
Proteinase K | 180 mg |
Protease Dissolve Buffer | 20 ml |
Buffer ATL | 300 ml |
Buffer PCI | 300 ml |
Buffer MLE | 180 ml |
Buffer GW1* | 132 ml |
Elution Buffer | 60 ml |
Storage and Stability
MagPure Particles, RNase A and Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
MagPure Stool DNA Kit is specially designed for high throughput DNA extraction from stool samples. It can get high purity microbial DNA from stool samples (≤200mg). This kit is based on magnetic beads purification and unique inhibiting factors adsorption technology, no phenol-chloroform extraction or alcohol precipitation. It can adsorb humic acid and other inhibiting factors in the solution efficiently. DNA can be directly used for downstream applications such as PCR, Viral DNA testing, bacterial DNA testing, etc.
Norgen’s FFPE RNA Purification Kits provide a rapid method for the isolation and purification of total RNA (including microRNA) from formalin-fixed paraffin-embedded (FFPE) tissue samples in as little as 1 hour. Using formalin to fix tissues leads to crosslinking of the RNA and proteins, and the process of embedding the tissue samples can also lead to fragmentation of the RNA over time. Norgen’s FFPE RNA Purification Kits provide conditions that allow for the partial reversing of the formalin modifications, resulting in a high quality and yield of RNA. These kits are able to purify all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA), depending on the age of the FFPE tissue as fragmentation of the RNA is known to occur over time. The RNA is preferentially purified from other cellular components without the use of phenol or chloroform.
FFPE RNA Purification Kit (Spin Column)
Maximum loading volume of 650 μL per column, and a maximum binding capacity of 50 μg per column.
FFPE RNA Purification 96-Well Kit (High Throughput)
Purification is based on 96-well column chromatography using Norgen’s proprietary resin as the separation matrix. Purification can be performed using either a vacuum manifold or centrifugation. Maximum loading volume of 400 μL per well, and a maximum binding capacity of 50 μg per well.
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Kit Specifications | |
Maximum Column Binding Capacity | Up to 50 µg RNA |
Maximum Loading Volume Per Spin Column | 650 µL |
Size of RNA Purified | All sizes, including small RNA (< 200 nt) |
Time to Complete 10 Purifications | 1-4 hours* |
Maximum Amount of Starting Material | 5 slices of < 20 µm thick paraffin slices 25 mg of unsectioned block |
Average Yield | Variable due to age of paraffin blocks ~2-3 µg of Total RNA per 1 mg of fresh FFPE hamster kidney |
* Time required for purification varies by length of Proteinase K incubation and formalin crosslink-reversal
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The DNAse I and Proteinase K should be stored at -20°C upon arrival. This kit is stable for 1 year from the date of shipment.
Component | Cat. 25300 (50 preps) | Cat. 25400 (2 x 96 preps) |
---|---|---|
Digestion Buffer A | 25 mL | 2 x 25 mL |
Buffer RL | 30 mL | 2 x 30 mL |
Enzyme Incubation Buffer | 6 mL | 2 x 6 mL |
Wash Solution A | 38 mL | 2 x 38 mL |
Elution Solution A | 6 mL | 2 x 20 mL |
Proteinase K | 12 mg | 2 x 20 mg |
DNase I | 1 vial | 2 x 500μL |
Micro Spin Columns | 50 | – |
96-Well Incubation Plate | – | 2 |
96-Well Plate | – | 2 |
Adhesive Tape | – | 8 |
Collection Tubes | 50 | – |
96-Well Collection Plate | – | 2 |
Elution Tubes (1.7 mL) | 50 | – |
96-Well Elution Plate | – | 2 |
Product Insert | 1 | 1 |
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection of numerous significant pathogens. The genesigPLEX range provides the most efficient method of detecting DNA and RNA by combining multiple assays into a single tube. This principle of multiplexing represents state of the art PCR testing and is a key feature of the high-throughput testing conducted by laboratories around the world. These assays include process controls to verify the quality of the nucleic acid extraction and eliminate false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit.
Details of the target and priming specificity are included in the handbook.
Packaged, optimised and ready to use. Expect Better Data.
Precise reproducible results
Time saving through completing multiple tests in one go
Cost saving through a reduced price compared with buying four kits separately
Exceptional value for money
Supplied lyophilised – no cold chain shipping
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Tel : 081-875-1869 , 02-328-7179
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