K-FRUCHK
SKU: 700004286
50 assays per kit
| Content: | 50 assays per kit |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | Fructan |
| Assay Format: | Spectrophotometer |
| Detection Method: | Absorbance |
| Wavelength (nm): | 340 |
| Signal Response: | Increase |
| Linear Range: | 4 to 80 µg of D-glucose, D-fructose or sucrose per assay |
| Limit of Detection: | 1 g/100 g |
| Total Assay Time: | ~ 30 min |
| Application examples: | Flours, plant materials (e.g. onion), food products and other materials |
The Fructan HK test kit is suitable for the specific measurement and analysis of all fructo-oligosaccharides (reducing and non-reducing) and of fructan polysaccharides but is not suitable for the analysis of samples containing high levels of D-glucose, D-fructose, sucrose or maltose.
View our full range of assay kits for polysaccharides.
Advantages
The Fructan HK test kit is suitable for the specific measurement and analysis of all fructo-oligosaccharides (reducing and non-reducing) and of fructan polysaccharides but is not suitable for the analysis of samples containing high levels of D-glucose, D-fructose, sucrose or maltose.
This product is suitable for rapid extraction of RNA from low RNA yield somples such as tissue (＜10mg), cells, bone marrow, fresh blood, and other clinical samples. RNA can be used directly for RT-PCR, Real time PCR, NGS, Viral RNA detection and so on.
Specifications
| Features | Specifications |
| Main Functions | Isolation total RNA from tissue, cell, whole blood |
| Applications | RT-PCR, cDNA synthesis, second generation sequencing |
| Purification method | Polydisperse magnetic beads |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Adaptive instrument | Nucleic acid extractor, pipetting workstation |
| Sample type | Tissues, cells, lymphocytes and other clinical sample |
| Sample amount | Cells grown in suspension: ≤3 x 106Animal tissue: ≤10mgPlant tissue: ≤30mgWhole blood: 0.5~1.0 ml fresh blood or bone marrow and fresh blood mixture |
Kit Contents
| Contents | IVD3020B-96 | IVD3020B |
| Purification Times | 96 Preps | 200 Preps |
| MagPure Particles N | 2.5 ml | 5 ml |
| DNase I | 2 x 600 µl | 4 x 600 µl |
| DNase Buffer C | 60 ml | 120 ml |
| Buffer RLC | 60 ml | 120 ml |
| Buffer MCB* | 60 ml | 150 ml |
| Buffer GW1* | 44 ml | 110 ml |
| Buffer MW2* | 50 ml | 100 ml |
| RNase Free Water | 20 ml | 60 ml |
Storage and Stability
DNase I should be shipped with ice pack and stored at -20°C after arrival. MagPure Particles N should be stored at 2–8°C for long time storage. The remaining kit components can be stored at room temperature(15–25°C) for up to18 months under these conditions.
This product is suitable for rapid extraction of RNA from low RNA yield somples such as tissue (＜10mg), cells, bone marrow, fresh blood, and other clinical samples. RNA can be used directly for RT-PCR, Real time PCR, NGS, Viral RNA detection and so on.
These kits provide a rapid method for the isolation and purification of total DNA from a wide range of plant and fungal species. Total DNA, including genomic DNA, mitochondrial DNA and chloroplast DNA can be purified from fresh or frozen plant tissues, plant cells or fungi samples using this kit. Purified DNA samples can be used for the detection of viral pathogens, as viral DNA is isolated with the plant/fungi DNA. The purified DNA is of the highest integrity, and can be used in a number of downstream applications including PCR, qPCR, SNP, Southern blotting and sequencing.
Plant/Fungi DNA Isolation Kit (Spin Column)
Complete 10 purifications in 45 minutes. This kit offers a maximum loading volume of 650 μL per column, and a maximum binding capacity of 50 μg per column.
Plant/Fungi DNA Isolation 96-Well Kit (High Throughput)
For high throughput applications. Purification with the 96-well plates can be integrated with a robotic automation system. Complete 96 purifications in 50 minutes. This kit offers a maximum loading volume of 500 μL per well, and a maximum binding capacity of 50 μg per well.
Plant/Fungi DNA Isolation Kit (Magnetic Bead System)
The DNA is bound to the surface of the magnetic beads under optimized buffer conditions and released using a low salt buffer system. The Plant DNA Isolation Kit (Magnetic Bead System) can be easily adapted to automated magnetic bead separation instruments and work stations.
Plant/Fungi DNA Isolation 96-Well Kit (High Throughput Magnetic Bead System)
For high throughput applications. Purification with the 96-well plates can be integrated with a robotic automation system.
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| Kit Specifications – Spin Column | |
| Column Binding Capacity | 50 μg |
| Maximum Column Loading Volume | 650 μL |
| Maximum Amount of Starting Material:Plant TissuesFungi (wet weight) | 100 mg 100 mg |
| Average Yields* 50 mg Tomato Leaves 50 mg Grape Leaves 50 mg Peach Leaves 50 mg Plum Leaves 50 mg Pine Needles Botrytis cinerea (50 mg wet weight) Fusarium sp. (50 mg wet weight) Aspergillus niger (50 mg wet weight) | 18 µg 10 µg 10 µg 10 µg 5 µg 1.5 µg 2 µg 4 µg |
| Time to Complete 10 Purifications | 45 minutes |
* Average yields will vary depending upon a number of factors including species, growth conditions used and developmental stage.
* Average yields will vary depending upon a number of factors including species, growth conditions used and developmental stage.
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature, except for the RNAse which should be stored at -20°C. This kit is stable for 1 year after the date of shipment.
Select Plants and Fungi that can be used with the Plant/Fungi DNA Purification Kits
| Plants | Plants (Cont’d) | Fungi |
| Tomato | Turnip | Aspergillus niger |
| Grape | Chinese Cabbage | Mucor racemosus |
| Peach | Radish | Cladosporium cladosporioides |
| Plum | Komatsuna | Fusarium oxysporum |
| Pine Needles | Apricot | Penicillium sp. |
| Raspberry | Sweet Potato | Botrytis cinerea (Botryotinia fuckeliana) |
| Strawberry | Hydrangea | Pichia sp. |
| Legumes | Fig | Rhizopus oryzae |
| Prosopis cineraria (Ghaf) | Turf | Alternaria tenuissima |
| Sorghum grass | Cherry | Fusarium sp. |
| Tobacco | Saintpaulia | |
| Arabidopsis | Lotus | |
| Lichen | Carrot | |
| Corn seed | Hansen | |
| Sunflower seed | Pistachio | |
| Olive seed | ||
| Soybean seed |
| Component | Cat. 26200 (50 preps) | Cat. 26250 (250 preps) | Cat. 26900 (192 preps) | Cat. 58200 (50 preps) | Cat. 62400 (192 preps) |
|---|---|---|---|---|---|
| Lysis Buffer L | 30 mL | 1 x 30 mL 2 x 60 mL | 2 x 60 mL | 60 mL | 2 x 60 mL |
| Binding Buffer I | 7 mL | 1 x 7 mL 1 x 25 mL | 25 mL | 7 mL | 25 mL |
| Solution WN | 18 mL | 1 x 18 mL 1 x 55 mL | 55 mL | 18 mL | 55 mL |
| Wash Solution A | 38 mL | 2 x 38 mL | 2 x 38 mL | – | – |
| Elution Buffer B | 15 mL | 30 mL | 30 mL | 8 mL | 30 mL |
| RNAse A | 1 vial (80 μL) | 5 vials (5 x 80 μL) | 1 vial | 1 vial | 1 vial |
| Magnetic Bead Suspension | – | – | – | 4 x 1.1 mL | 2 x 8.5 mL |
| Filter Columns | 50 | 250 | – | – | – |
| Spin Columns | 50 | 250 | – | – | – |
| Collection Tubes | 100 | 500 | – | – | – |
| 96-Well Plate | – | – | 2 | – | 2 |
| 96-Well Collection Plate | – | – | 2 | – | – |
| Adhesive Tape | – | – | 4 | – | 2 |
| Elution Tubes (1.7 mL) | 50 | 250 | – | 50 | – |
| 96-Well Elution Plate | – | – | 2 | – | 2 |
| Product Insert | 1 | 1 | 1 | 1 | 1 |