Candida albicans is an opportunistic yeast and is the most common fungal pathogen found in the human body. C. albicans can be detected in the gastrointestinal tract, mouth, and vagina of approximately half of healthy adults. It is typically a commensal organism and makes up part of the natural human microflora; however it can overgrow and become pathogenic as a result of various conditions. For example, individuals who have taken recent courses of antibiotics, have a weakened immune system or have diabetes have an increased risk of developing a Candida albicans infection. When an overgrowth occurs, this can lead to common infections such as urinary tract infections, genital yeast infections, oral thrush, and mucocutaneous candidiasis. In the more severe cases, when Candida albicans enters the bloodstream or organs, it can lead to loss of sight, blood and bone infections, endocarditis, meningitis or inflammation of the intraabdominal lining.
Figure 1 / 3
Click for expanded view
Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival.
| Component | Cat. TM34050 (100 preps) | Cat. TM34010 (100 preps) |
|---|---|---|
| MDx TaqMan 2X PCR Master Mix | 2 x 700 μL | – |
| Candida albicans Primer & Probe Mix | 280 μL | 280 μL |
| Candida albicans Positive Control | 150 μL | 150 μL |
| Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
| Product Insert | 1 | 1 |
Blood samples contain rich DNA, including mitochondrial DNA, genomic DNA, circulating DNA (mostly released into blood after tumor cell apoptosis) in white blood cells, as well as parasitic viral or microbial DNA. These DNA are important parameters in clinical testing or diagnosis, which are also valuable materials for medical research. There are three main issues with extracting DNA from blood samples:
1. The sample is highly infectious, posing great harm to operators and the environment.
2. The source of DNA is complex and aportion of the nucleic acid, such as viral DNA or free DNA, may be lost during the operation, leading to downstream detection failure;
3. Blood sample contains a large amount of impurities and inhibitory factors.
Currently there are many methods available for extracting DNA from whole blood samples, such as phenol chloroform extraction, salting out method, etc. However, these methods require pre-treatment of blood sample, which removes red blood cells and isolate white blood cells in the first step. Due to the requirement that it cannot inactivate or kill pathogens during the process of removing red blood cells, the waste liquid (red blood cell lysate) and consumables may be contaminated by pathogens and become infectious, posing a danger to the entire laboratory environment and operators. In addition, during the process of removing red blood cells, useful nucleic acid information such as viruses, microorganisms, or circulating DNA is also lost, leading to experiment or detection failures.
The HiPure Blood DNA Kits series provided by Magen Company uses silica gel column purification technology, which can directly lyse whole blood samples without the need for white blood cell separation. Whole blood samples are directly mixed with lysates and proteases, resulting in the inactivation of pathogens, greatly reducing the infectivity, environmental pollution, and the chance of operators being infected. Due to the direct lysis and digestion of samples, except lymphocyte DNA, other circulating DNA as well as DNA from viruses and microorganisms, can also be recovered.
This product provides fast and easy methods for purification of total DNA for reliable PCR and Southern blotting. Total DNA (e.g., genomic, viral, mitochondrial) can be purified from whole blood, tissue and culture cells.
Details
Specifications
| Features | Specifications |
| Main Functions | Isolation total DNA from 10ml blood and 1g tissue using Maxi column |
| Applications | PCR, southern bolt and virus detection, etc |
| Purification method | Maxi spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Tissue, cell, blood, saliva, swab, blood spot, semen and other clinical samples |
| Sample amount | 3-10ml |
| Elution volume | ≥700μl |
| Time per run | ≤90 minutes |
| Liquid carrying volume per column | 4ml |
| Binding yield of column | 5mg |
This product is based on silica column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
Kit Contents
| Contents | D311502 | D311503 |
| Purification Times | 10 | 50 |
| HiPure gDNA Maxi Columns | 10 | 50 |
| 50ml Collection Tubes | 20 | 100 |
| Buffer ATL | 120 ml | 550 ml |
| Buffer AL | 120 ml | 2 x 300 ml |
| Buffer GW1* | 53 ml | 220 ml |
| Buffer GW2* | 25 ml | 110 ml |
| RNase A | 40 mg | 180 ml |
| Proteinase K | 120 mg | 540 mg |
| Protease Dissolve Buffer | 12 ml | 50 ml |
| Buffer AE | 30 ml | 120 ml |
Storage and Stability
Proteinase K, RNase A should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
| Name | CAT NO | Sample amount | Leukocyte protocol* | Colum type | Elutio volume | Average yield | Time per run |
| HiPure Blood DNA Mini Kit | D3111 | 10-200μl | 1ml | 2ml column | ≥20μl | 5-9μg/200μl | ≤30 minutes |
| HiPure Tissue&Blood DNA Midi Kit | D3113 | 0.2-2ml | 10ml | 1.5ml column | ≥300μl | 20-40μg/1m | ≤80 minutes |
| HiPure Tissue&Blood DNA Maxi Kit | D3115 | 3 -10ml | 10ml | 15ml column | ≥700μl | 20-40μg/1m | ≤90 minutes |
| HiPure Tissue&Blood DNA 96 Kit | D3117 | 1-200μl | 1ml | 96 well plate | 3-8μg/200μl |
*Note：Leukocyte protocol can be used when large volume whole blood samples need to be processed. Whole blood was treated with red blood cell lysate, and white blood cells were obtained by centrifugation before extraction
Blood samples contain rich DNA, including mitochondrial DNA, genomic DNA, circulating DNA (mostly released into blood after tumor cell apoptosis) in white blood cells, as well as parasitic viral or microbial DNA. These DNA are important parameters in clinical testing or diagnosis, which are also valuable materials for medical research. There are three main issues with extracting DNA from blood samples:
Description
The ExcelDye™ 6× DNA Loading Dye (Orange) is pre-mixed buffer for tracking the DNA sample during the electrophoresis on agarose or polyacrylamide gels. It contains one dye (Orange G) for tracking the DNA migration. The Orange G migrates at approximately 30 bp on a standard 2% TAE agarose gel (50 bp on 1% TAE agarose gel). The included glycerol keeps the DNA at the bottom of the well and the presence of EDTA chelates divalent metal ions to prevent the process of metal-dependent nuclease.
Composition
Storage
4°C for 12 months
-20°C for 36 months
The ExcelDye™ 6× DNA Loading Dye (Orange) is pre-mixed buffer for tracking the DNA sample during the electrophoresis on agarose or polyacrylamide gels. It contains one dye (Orange G) for tracking the DNA migration. The Orange G migrates at approximately 30 bp on a standard 2% TAE agarose gel (50 bp on 1% TAE agarose gel). The included glycerol keeps the DNA at the bottom of the well and the presence of EDTA chelates divalent metal ions to prevent the process of metal-dependent nuclease.