This kit provides a rapid spin column procedure for the purification and clean-up of sequencing and various other enzymatic reactions including restriction enzyme digests, Klenow reactions, alkaline phosphatase reactions, and ligations. The kit is used to remove reaction contaminants including dye terminators, salts, enzymes, excess primers and primer dimers. Contaminants are undesirable as they can interfere with many downstream applications including sequencing, RFLP, restriction enzyme digestions and ligation. Purification is based on spin-column chromatography without the use of phenol, chloroform or alcohol precipitation. The kit provides a high quality product with up to 90% recovery.
The kit is also available in a 96-well format for high-throughput sequencing reaction clean-up. Purification with the 96-well plate can be performed using either a vacuum manifold or centrifugation.
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| Kit Specifications – 96-well | |
| Binding Capacity Per Well | 15 μg |
| Maximum Loading Volume Per Well | 500 μL |
| Average DNA Recovery | Up to 90% |
| Time to Complete 96 Purifications | 20 minutes |
| Minimum Elution Volume | 50 μL |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
| Component | Cat. 34500 (50 preps) | Cat. 34400 (2 x 96-well plate) |
|---|---|---|
| Binding Buffer C | 30 mL | 3 x 30 mL |
| Wash Solution A | 12 mL | 2 x 20 mL |
| Elution Buffer B | 8 mL | 2 x 15 mL |
| Spin Columns | 50 | – |
| Collection Tubes | 50 | – |
| Elution Tubes (1.7 mL) | 50 | – |
| 96-Well Plate | – | 2 |
| Adhesive Tape | – | 4 |
| 96-Well Collection Plate | – | 2 |
| 96-Well Elution Plate | – | 2 |
| Product Insert | 1 | 1 |
This kit provides a fast, reliable and convenient method to isolate and concentrate exosomal RNA from previously purified exosomes. It allows for the isolation of RNA from intact extracellular vesicles (EVs) purified from different urine or plasma/serum sample volumes. The purification is based on Norgen’s proprietary resin.
The Exosomal RNA Isolation Kit is designed to isolate all sizes of RNA, including microRNA. Moreover, the kit allows the user to elute into a flexible elution volume ranging from 50 µL to 100 µL. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.
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| Kit Specifications | |
| Sample type | Exosomes purified using Norgen’s Purification Kits and most other methods |
| Size of RNA Purified | All sizes, including miRNA and small RNA (< 200 nt) |
| Elution Volume | 50-100 μL |
| Time to Complete 10 Purifications | 35-40 minutes |
| Average Yields* | Variable depending on specimen |
*Please check page 5 of the product insert for the average yields and the common RNA quantification methods.
Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.
| Component | Cat. 58000 (50 preps) |
|---|---|
| Lysis Buffer A | 20 mL |
| Lysis Additive B | 2 mL |
| Wash Solution A | 18 mL |
| Elution Solution A | 6 mL |
| Mini Spin Columns | 50 |
| Collection Tubes | 50 |
| Elution Tubes (1.7 mL) | 50 |
| Product Insert | 1 |
1.This series of products was filling at A level of environment, and final sterilization by Gamma ray irradiation, triple packaging insure sterility and long shelf life.
2.Each dish was marking label product name, batch number, expiration date .Information is available of traceability.
3.Inner additional desiccant, reducing formation of condensation water, while inner additional sterile paper and plastic bags for easier transfer and cultivation.
4.Triple packing dense bags to avoid the penetration of hydrogen peroxide; clean gas was filled as a buffer to reduce broken bags and dish in transit.
5.The 90mm medium plate loading capacity of 30mL, to meet requirement of the environmental sampling for long exposure of up to 4h and 5-7 days of culture.
6. This series of products is available to store at (2-25 ℃), shelf life of up to 6 months.
90mm*10 plates / bag