The genesig® Real-Time PCR COVID-19 (CE) is CE-IVD marked and intended for in vitro diagnostic use in Europe.
PLEASE NOTE: This is a Professional Use Only product that requires a trained operator in a controlled laboratory environment. It is NOT a lateral flow or a Point of Care device designed to be used by the general public.
Total workflow solution
All reagents and components provided from start to finish
Rapid protocol with fewer handling steps
For use with dry swabs
Viral inactivation; Preparation, Amplification, Diagnosis
Total chemistry optimisation
Low shipping costs with ambient shipping
Includes our genesig® Real-Time PCR Coronavirus COVID-19 (CE IVD)
Norgen’s Clostridium difficile TaqMan PCR Lyophilized Kit is designed for the detection of Clostridium difficile specific DNA in a real-time PCR based on the use of TaqMan® technology. The lyophilized format is designed to ship the kit at ambient temperature.
Norgen’s Clostridium difficile TaqMan Lyophilized Probe/Primer and Control Set is designed for the detection of Clostridium difficile specific DNA in a real-time PCR based on the use of TaqMan® technology. The lyophilized format is designed to ship the kit at ambient temperature.
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| Component | Cat. TM37150L (100 preps) | Cat. TM37110L (100 preps) |
|---|---|---|
| MDx TaqMan 2X PCR Master Mix (Lyo) | 4 x 350 μL | – |
| Clostridium difficile Primer & Probe Mix (Lyo) | 280 μL | 280 μL |
| Clostridium difficile Positive Control (Lyo) | 150 μL | 150 μL |
| Nuclease-Free Water (Negative Control) | 3 x 1.25 mL | 1 x 1.25 mL |
| Product Insert | 1 | 1 |
Not all cyanobacterial strains produce toxins. However, the toxin-producing strains cannot be distinguished from the nontoxin-producing strains by traditional light microscopy, commonlyused to monitor water bodies. An alternative for the differentiation of potentially toxic strains from nontoxic strains is to use molecular methods to detect the presence of toxin biosynthetic genes. Such methods are already available and could be used for the detection and identification of potential microcystin and nodularin producers present in environmental samples (Attogene catalog number NA2024).
Screening for the toxin itself, can be very costly. In turn, real time PCR for the detection of a gene region responsible for assembling in cyanobacterial strains and environmental samples can be a key indicator for the prescense of cyanobacteria capable of expressing the aetokthonotoxin toxin. Attogen has thus, designed primer pairs and probes targeting a the conserved gene region in order to enable the amplification and detection of several producer genera using real time PCR. Screening for the toxin genes can save significant costs and act as a triage for samples needing to be analyzed for the toxin itself.
Cyanobacterial neurotoxin aetokthonotoxin (AETX), a peculiar pentabrominated biindole alkaloid implicated in fatal Vacuolar Myelinopathy. This neurodegenerative disease was first recorded in 1994 during an outbreak of bald-eagle poisonings at De Gray Lake in Arkansas, USA. AETX was experimentally confirmed to be produced by the true branching heterocytous cyanobacterium Aetokthonos hydrillicola. The production of AETX is dependent on bromide (Br−) availability, and likely linked to its hyper-accumulation by the host plan. Thus regular monitoring of A. hydrillicola (accompanied by assessment of Br− and AETX levels) is highly advisable to predict the possible threat of further VM outbreaks.
The cyanobacterial AetA gene which encodes the unique FAD-dependent halogenase involved in the pathway for AETX synthesis has been adapted to develop a -aetokthonotoxin specific quantitative PCR (qPCR) assay.
Real time qPCR kit for AetA gene
For screening aetokthonotoxin gene cluster
Use in combination with Attogene Algae DNA isolation kit