Features
1.Brushless frequency motor, in great torque, free maintenance, no powder pollution, quick in speed up and down.
2.Imported compressors fluorine free,double cycle cooling, cold and hot alternating easily, free environment pollution and precise in temperature control.
3.Microprocessor control, digital display which indicates the temperature, speed, time, RCF in operation, speed raising and reducing quickly, operate simply. Running parameter can be edited.
4. 10 kinds of program stored in the memory, 10 kinds of accelerating and decelerating speed for your choice.
5. Automatically electric lid lock, over speed, over temperature protection and imbalance protection.
6.High quality steel centrifuge body , stainless steel chamber, built-in stainless steel explosion-proof protect sleeve , 3 tiers protection,safe and reliable.
7. Rotor is connected to spindle by specialized spring taper sleeve, loading simple and quick, no direction
8. With pre-cooling and get the ideal centrifugation result.
TGL16 Technical Parameter：
| Max. Speed | 16000rpm |
| Max. RCF | 20600×g |
| Max. Capacity | 6×100ml |
| Time Range | 1~9h59min |
| RPM/RCF Convert | Yes |
| Noise (dB) | ≤ 58 |
| Acc/Dec | 10 Kinds |
| Speed Accuracy | ±20r/min |
| Temperature Accuracy | ±1℃ |
| Voltage(V/Hz) | AC 220V/110V 50HZ/60HZ |
| Size (L x W x Hmm) | 610×570×370mm |
| Net Weight(Kg) | 82KG |
| Certificates | TUV CE,ISO 9001,ISO13485 & Calibration report are available |
Matched Rotor for TGL16
| Order No | Rotor | Max speed (rpm) | Max Volume(ml) | Max RCF (g) |
| G16-1 | Angle rotor | 14000 | 4×8PCR | 12070 |
| G16-2 | Angle rotor | 13000 | 6×8PCR | 16080 |
| G16-3 | Angle rotor | 14000 | 8×8PCR | 13390 |
| G16-4 | Angle rotor | 13000 | 12×8PCR | 17220 |
| G16-5 | Angle Rotor | 15000 | 30×0.5ml | 18510 |
| G16-6 | Angle Rotor | 14000 | 40×0.5ml | 19970 |
| G16-7 | Angle Rotor | 16000 | 12×1.5/2ml | 17940 |
| G16-8 | Angle Rotor | 15000 | 24×1.5/2ml | 20600 |
| G16-9 | Angle Rotor | 13500 | 30×1.5/2ml | 19340 |
| G16-10 | Angle Rotor | 11000 | 48×1.5/2ml | 12840 |
| G16-11 | Angle Rotor | 15000 | 16×5ml | 19350 |
| G16-12 | Angle Rotor | 14000 | 12×7ml | 16370 |
| G16-13 | Angle Rotor | 14000 | 6×10ml | 16460 |
| G16-14 | Angle Rotor | 12000 | 12×10ml | 14510 |
| G16-15 | Angle Rotor | 10000 | 12×15ml | 11840 |
| G16-16 | Angle Rotor | 5000 | 24×15ml | 3080 |
| G16-17 | Angle Rotor | 5000 | 30×15ml | 3830 |
| G16-18 | Angle Rotor | 12000 | 8×20ml | 14510 |
| G16-19 | Angle Rotor | 12000 | 6×30ml | 14000 |
| G16-20 | Angle Rotor | 11000 | 6×50ml | 13480 |
| G16-21 | Angle Rotor | 12000 | 6×70ml | 10810 |
| G16-22 | Angle Rotor | 8000 | 6×100ml | 7280 |
| G16-23 | Angle Rotor | 12000 | 24 pieces capillary vessel | 15800 |
| G16-24 | Microplate rotor | 4000 | 2×3×48 Well | 2300 |
Norgen’s Plasma/Serum Exosome and RNA Isolation Kits constitute all-in-one systems for the purification of exosomes and the sequential isolation of exosomal RNA from different plasma/serum sample volumes. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. These kits are designed to isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias. These kits provide a clear advantage over other available kits in that they do not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. The RNA isolated from the purified exosomes is free from any protein-bound circulating RNA and is of the highest integrity. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.
Norgen’s Plasma/Serum Exosome and RNA Isolation Mini Kit
For sample volumes ranging from 50 µL to 1 mL. This kit allows the user to elute into a flexible elution volume ranging from 50 µL to 100 µL.
Norgen’s Plasma/Serum Exosome and RNA Isolation Midi Kit
For sample volumes ranging from 1 mL to 4 mL. This kit allows the user to elute into a flexible elution volume ranging from 50 µL to 100 µL.
Norgen’s Plasma/Serum Exosome and RNA Isolation Maxi Kit
For sample volumes ranging from 4 mL to 10 mL. This kit allows the user to elute into a flexible elution volume ranging from 50 µL to 100 µL.
Figure 1 / 5
Click for expanded view
| Kit Specifications | |
| Minimum Plasma/Serum Input | 50 μL |
| Maximum Plasma/Serum Input | 1 mL |
| Size of Exosomes Purified | 40 nm – 150 nm |
| Size of RNA Purified | All sizes, including miRNA and small RNA (< 200 nt) |
| Elution Volume | 50-100 μL |
| Time to Complete 10 Purifications | 35 – 40 minutes |
| Average Yields* | Variable depending on specimen |
*Please check page 4 of the product insert for the average yields and the common RNA quantification methods.
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.
Important Note
This kit is suitable for the purification of exosomes from fresh or frozen serum or plasma prepared from blood collected on either EDTA or Citrate. Plasma samples prepared from blood collected on heparin should not be used as heparin can significantly interfere with many downstream applications such as RT-PCR.
| Component | Cat. 58300 (50 preps) | Cat. 58500 (25 preps) | Cat. 58600 (15 preps) |
|---|---|---|---|
| Slurry E | 12.5 mL | 12.5 mL | 12.5 mL |
| ExoC Buffer | 8 mL | 8 mL | 2 x 8 mL |
| ExoR Buffer | 12 mL | 12 mL | 12 mL |
| Lysis Buffer A | 20 mL | 20 mL | 20 mL |
| Lysis Additive B | 2 mL | 2 mL | 2 mL |
| Wash Solution A | 38 mL | 18 mL | 18 mL |
| Elution Solution A | 6 mL | 6 mL | 6 mL |
| Mini Filter Spin Columns | 50 | 25 | 15 |
| Mini Spin Columns | 50 | 25 | 15 |
| Collection Tubes | 50 | 25 | 15 |
| Elution Tubes (1.7 mL) | 100 | 50 | 30 |
| Product Insert | 1 | 1 | 1 |
| Clone | IHC002 |
| Source | Mouse Monoclonal |
| Positive Control | Breast Carcinoma |
| Dilution Range | 1:200 |
The Her2/Neu (c-erbB-2) proto-oncogene is a transmembrane receptor tyrosine kinase that is clinically indicated in a number of carcinomas. Overexpression of the c-erbB-2 protein has been associated with ductal breast cancer, as well as pulmonary and gastric adenocarcinomas. A correlation between Her2 and p53 has also been documented, as overexpression of both proteins has been associated with early invasion and metastasis in bladder cancer.