This kit provides a rapid method for the isolation and purification of both cytoplasmic and nuclear RNA from cultured animal cells and small tissue samples. The kit can be used to isolate all sizes of RNA from the cytoplasmic and nuclear RNA fractions, including all small RNA species without any requirement for phenol. Included in the kit are sufficient reagents to perform either 50 cytoplasmic RNA preparations or 25 cytoplasmic and 25 nuclear RNA preparations. Ten samples can be processed in approximately 45 minutes. This kit is also available in a 100 prep size.
Background
In certain circumstances it is desirable to be able to isolate fractionated RNA as opposed to total RNA. For example, it may be preferable to isolate only mature, cytoplasmic RNA for some studies on expression profiling. Alternatively it may be desirable to isolate nuclear RNA in order to investigate and study pre-processed (non-spliced) RNA. Furthermore, this kit can be used to isolate RNA for downstream applications where it is necessary to avoid DNA contamination, since the cytoplasmic fraction has been shown to be free of all traces of genomic DNA.
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| Kit Specifications | |
| Maximum Column Binding Capacity | Up to 50 µg RNA |
| Maximum Column Loading Volume | 650 µL |
| Size of RNA Purified | All sizes, including small RNA (< 200 nt) |
| Time to Complete 10 Purifications | 45 minutes |
| RNA Yield HeLa (1 x 106) – Cytoplasmic RNA HeLa (1 x 106) – Nuclear RNA | 15 µg ≤ 3.5 µg |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
| Component | Cat. 21000 (50 preps) | Cat. 37400 (100 preps) |
|---|---|---|
| Lysis Buffer J | 20 mL | 2 x 20 mL |
| Buffer SK | 40 mL | 2 x 40 mL |
| Wash Solution A | 38 mL | 2 x 38 mL |
| Elution Buffer E | 6 mL | 2 x 6 mL |
| Mini Spin Columns | 50 | 100 |
| Collection Tubes | 50 | 100 |
| Elution Tubes (1.7 mL) | 50 | 100 |
| Product Insert | 1 | 1 |
Name of Product
Hop Resistance Gene-Screen
Catalog Number
MGScHOR 1
Short Info
This test discovers hop resistance genes in a timely and precise manner
Method/Platform
PCR
Range/Assay Sensivity
10^4 – 10^5 cfu/mL
Test Principle
The technological basis for the GenLine tests is the polymerase chain reaction (PCR) combined with lateral flow Tests.
Labelled specific primers are used to amplify specific DNA fragments. In addition to the target gene, a control gene, which is also present in the PCR mixes, is amplified in order to make sure that the PCR process works properly.
The resulting PCR products carry the labels of the incorporated primers.
In a second part of the test, the created PCR products are detected by a lateral flow Test Strip. A “molecular sandwich” is formed and becomes visible as a line on the test Strip.
Brief Instructions
Storage
-15 to -20°C
Components
PCR-Polymerase Master-Mix, PCR-Primermix, Positive Kit-Control, PCR-Water
Name of Product
Hop Resistance Gene-Screen
Catalog Number
MGScHOR 1
Short Info
This test discovers hop resistance genes in a timely and precise manner
Method/Platform
PCR
Range/Assay Sensivity
10^4 – 10^5 cfu/mL
Test Principle
The technological basis for the GenLine tests is the polymerase chain reaction (PCR) combined with lateral flow Tests.
Norgen’s RNA purification kits isolate total RNA with minimal amounts of genomic DNA contamination. However, for some sensitive downstream applications, it may be desirable to remove all traces of residual DNA. Norgen’s RNase-free DNAse I Kit, with Enzyme Incubation Buffer, can be used for optional on-column DNase digestion with any of Norgen’s RNA purification kits. Alternatively, after isolating total RNA using one of Norgen’s RNA purification kits, the RNA elution can be treated with this DNase I. The RNA can then be purified from the DNase using Norgen’s RNA Clean-Up and Concentration Kit (Cat# 23600), and the RNA can then be used in downstream applications.
Each RNase-Free DNase I Kit is supplied complete with sufficient enzyme and enzyme incubation buffer for 50 or 200 reactions.
Storage Conditions
The DNase I provided is in lyophilized form. It is stable for at least 3 months if stored at room temperature. However, it is recommended to store the DNase I vial at 2 – 8ºC (or below) upon receipt to maintain stability beyond 3 months. Buffer DR and Enzyme Incubation Buffer can be stored at room temperature. After reconstitution with Buffer DR (see product manual), the DNase I should be stored at -20ºC. All reagents should remain stable for at least 1 year in their unopened containers at the appropriate storage temperature.
| Component | Cat. 25710 (50 rxns) | Cat. 25720 (200 rxns) |
|---|---|---|
| DNase I | 1 vial / 1,600 units | 4 vials (1,600 units/vial) |
| Buffer DR | 1 mL | 4 x 1 mL |
| Enzyme Incubation Buffer | 6 mL | 4 x 6 mL |
| Product Insert | 1 | 1 |