

Detection of three specific pathogen targets and an internal control. Total input DNA per reaction was 10^6 (red), 10^5 (yellow), 10^4 (blue), 10^3 (green), 10^2 (purple), 10 (light blue) and 0 copies (grey) and 8000 copies/reaction for the internal control (shown in the CY5 plot).
PlexTaq 5x qPCR Multiplex Master Mix contains all components necessary for rapid, sensitive and reproducible quantification of DNA and cDNA. An engineered DNA polymerase and an optimized buffer including ultrapure dNTPs are key components of the ready to use mix. A hot-start formulation of the included DNA polymerase prevents aptamer prevents false amplification and provides a fast start function.
PlexTaq®´s formulation allows to use it also for direct PCRs from crude samples.
All in One. All the flexibility you need in a 5x Master Mix for multiplexing applications. NOW LYO READY!
+ Sensitive. More free volume. 5x concentration allows more volume for target specific primers and probes (multiplexing).
+ Robust. Uniform amplification. Up to 30 target multiplexing in real-time (customer feedback).
+ Fast TTR. No extraction needed. Reliable results with crude samples without extraction step, like blood.
+ Specific. No false amplification. Engineered Taq DNA polymerase more stable at room temperature. Aptamer-based hot-start prevents false amplification and provides a fast-start function.
Our SNPsig® kits use our own proprietary genotyping method to enable the identification of SARS-CoV-2 variants of concern. These products can be used on any real-time PCR machine using familiar protocols, whilst resulting in exceptional genotyping data.
Positive control templates for wild-type and variants are supplied in every kit to make data interpretation simple.
Our SNPsig® technology provides an alternative to sequencing as well as S gene target failure (SGTF) that enables scientists to analyse and monitor these specific genomic mutations. Our kits can provide a pivotal role in screening for SARS-CoV-2 variants for the purpose of genomic surveillance and studies.
For the detection of the SARS-CoV-2 variants with the L452R mutation
Rapid detection of specific detection profiles
High priming efficiency
Sensitive to < 100 copies of target
Positive copy number standard curve for quantification
Accurate controls to confirm findings
96 reactions, includes master mix
| Usage | For cultivating and enriching nofastidious bacteria ,and for evaluating the efficacy of disinfection. |
| Formula (per liter) | Peptone—————10.0g Beef Extract———–3.0g Sodium Chloride—–5.0g Final pH———–7.4 ± 0.2 |
| How to use | 1.Suspend 18g in 1L of distilled water , stirring heated to boiling to completely dissolve ,autoclave at 121ºC for 15 minutes. 2.Diluted and treated samples. |
| Storage | Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years. |
500g