160×7ml Swing Buckets Rotor 

Product Description

RNA Amplification Kit With Improved Detection & Quantification-20°C Format Easy And Efficient

RNA Amplification Kit With Improved Detection & Quantification-20°C Format Easy And Efficient

Product Detail

Kit Storage and term of Validity

Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.

Term of Validity: 14 months

Isothermal nucleic acid Principle Summary

This kit is based on a rapid nucleic acid amplification technology at room temperature and constant temperature: at room temperature and constant temperature (generally 39 ºC~42 ºC), reverse transcriptase uses specific primers and template RNA to synthesize cDNA strands, and binds the auxiliary protein and single strand With the help of the protein, the recombinase and the primer form a complex; perform a homology search and bind the target homology domain, at this time a D-loop region is formed at the homology position and strand exchange begins; accompanied by the recombinase from the complex Upon dissociation, the polymerase also binds to the 3′ end of the primer, initiating chain extension. Relying on the action of nfo enzyme, adding specific molecular probes designed according to the template, and using colloidal gold technology (sandwich method) can detect the final result.

Isothermal nucleic acid Product Features

1/ High sensitivity and specificity, short reaction time.

2/ The reagent form is freeze-dried, stable and easy to operate.

3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.

Technical Parameters:

Isothermal nucleic acid Applications

Suitable for RNA isothermal rapid amplification kit(NFO type)

Primer: Require pair of nucleotide primers with the length of 25-35 bp.

RNA NFO kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.

Notes

1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.

2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.

Propargyl-PEG8-amine is a heterobifunctional reagent consisting of a propargyl group and an amine group. The amine group can form amide bonds with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde) etc. The propargyl group can form triazole linkage with azides in copper catalyzed Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Propargyl-PEG8-amine is a heterobifunctional reagent consisting of a propargyl group and an amine group. The amine group can form amide bonds with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde) etc. The propargyl group can form triazole linkage with azides in copper catalyzed Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Introduction

Usages：
For determination of coliform confirmatory test for multi-tube fermentation.
 
Principle:
Peptone provide carbon and nitrogen sources; lactose as fermentable sugars; ox bile powder and brilliant green inhibit the non-Enterobacteriaceae.
 
Formulation (per liter):
Peptone: 10g
Lactose: 10g
Ox bile powder: 20g
Brilliant green: 0.0133g
Final pH7.2 ± 0.2
 
How to use:
1.Suspend 40g of the product, add 1 L of distilled water, stirring heated to boiling until completely dissolved, packed in a test tube with a small inverted tube, autoclave at 121 ℃ for 15min,  cool to room temperature and set aside.
2.Sample handling and dilution. Slightly.
3.Transplanted LST broth tube to brilliant green lactose bile broth tube by using a inoculating loop.
4.the tube was placed in an incubator culture at 36 ± 1 ℃ for 24h.
5.Observe the results. Press production trachea number, check table report MPN MPN value per gram (mL) sample of coliform bacteria.
 
Quality control:
Quality control strains were inoculated and culuture at 36 ± 1 ℃ for 24h ,results are as follows:
Bacterial Name                 Bacterial No.          Growth Status       Gassing
Escherichia coli                ATCC25922               good              +
 
Salmonella typhimurium        CMCC (B) 50115            good             —
 
Staphylococcus aureus          ATCC6538                inhibited          —
 
 
Storage: Store in a dark, cool and dry place, tighten the cap immediately after use. Storage period of three years.
 
Specifications: 250g/bottle

250g