160×7ml Swing Buckets Rotor 

Overview

• Fast and easy processing using rapid spin-column format
• Isolate high quality mitochondrial DNA
• Recovered DNA is compatible with various downstream applications

Over the past several decades, mitochondrial DNA (mtDNA) has played an increasingly important role in forensic analyses of various criminal cases. A few hairs left at a crime scene contain enough mtDNA for extraction. The hair shaft, which protrudes out of the scalp, does not contain any nuclear DNA. It does, however, contain mtDNA. While nuclear DNA is present in only two copies per cell, the small circular mtDNA molecule is present in hundreds to thousands of copies per cell making it very abundant. Mitochondrial DNA is maternally inherited, and all of a woman’s offspring will have the same mtDNA profile. An advantage of this is that a single maternal relative of that person may provide a reference sample for comparison to a sample found at a crime scene.

Norgen’s Hair Mitochondrial DNA Isolation Kit provides a fast, reliable, and simple procedure for isolating mtDNA from hair shafts. Purification is based on spin column chromatography and the DNA is preferentially purified from other components. Typical yields will vary depending on the sample input volume used. The purified DNA is compatible with all downstream applications including PCR and NGS.

Details

Supporting Data

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Storage Conditions and Product Stability
Store DTT at -20°C upon arrival. All other solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers. The kit contains a ready to-use Proteinase K solution, which is dissolved in a specially prepared storage buffer. The Proteinase K is stable for up to 2 years after delivery when stored at room temperature. To prolong the lifetime of Proteinase K, storage at 2–8°C is recommended.

Component	Cat. 69400 (50 preps)
DTT	6 mL
Proteinase K	4 mL
Lysis Additive A	6 mL
Lysis Buffer B	20 mL
Wash Solution A	38 mL
Elution Buffer B	8 mL
Micro Spin Columns	50
Elution Tubes	50
Collection Tubes	50
Product Insert	1

N-(t-butyl ester-PEG2)-N-bis(PEG2-propargyl) is a multi-functional PEG linker with two terminal propargyl groups and a t-butyl ester. The propargyl groups enables formation of triazole linkage with azide-bearing compounds or biomolecules in copper catalyzed Click Chemistry. The t-butyl protected carboxyl group can be deprotected under mild acidic conditions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

N-(t-butyl ester-PEG2)-N-bis(PEG2-propargyl) is a multi-functional PEG linker with two terminal propargyl groups and a t-butyl ester. The propargyl groups enables formation of triazole linkage with azide-bearing compounds or biomolecules in copper catalyzed Click Chemistry. The t-butyl protected carboxyl group can be deprotected under mild acidic conditions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Description 

The IdPath™ COVID-19 Real-Time RT-PCR Kit is a real-time RT-PCR test intended for the detection of SARS-CoV-2 virus RNA which might be extracted from the respiratory tract specimens. The Kit provides reagents for multiplex real-time RT-PCR to detect SARS-CoV-2 by one-step reaction, specifically targeting the E (Envelope), RdRP (RNA-dependent RNA polymerase) and N (Nucleocapsid protein) gene for SARS-CoV-2 virus.

The Kit contains the RT enzyme mix and qPCR master mix for reverse transcription and real-time PCR of virus RNA.  The COVID-19 Control (positive control) and ddWater (negative control) are used as indicators to avoid false negative/positive results across all experimental procedures. The Primers/probes Mix contains multiplex primers and TaqMan probes specific to the N, E/RdRP genes of SARS-CoV-2 and RNase P gene of human, detected by FAM, VIC and ROX channels, respectively.

For Research Use Only

Features

• High Sensitivity：5×10² copies/ml (10 copies/rxn)
• High Inclusivity : >99% of currently available complete virus genomes for SARS-CoV-2 including Omicron variant
• High Accuracy : Clinical validation with 100% accuracy
• High Stability : 37/25°C for 2 weeks ; 4°C for 24 weeks ; 10 times of freeze-thaw cycles
• High Compatibility : Suitable for most laboratory qPCR machines
• Operation Control : Including internal control for quality control of total process
• Convenience : Multiplex (E/RdRP and N) detection by one-step reaction

Storage

Aliquot to avoid multiple freeze-thaw cycles

Protect fluorogenic probes from light

-20°C for 12 months

The IdPath™ COVID-19 Real-Time RT-PCR Kit is a real-time RT-PCR test intended for the detection of SARS-CoV-2 virus RNA which might be extracted from the respiratory tract specimens. The Kit provides reagents for multiplex real-time RT-PCR to detect SARS-CoV-2 by one-step reaction, specifically targeting the E (Envelope), RdRP (RNA-dependent RNA polymerase) and N (Nucleocapsid protein) gene for SARS-CoV-2 virus.
The Kit contains the RT enzyme mix and qPCR master mix for reverse transcription and real-time PCR of virus RNA.  The COVID-19 Control (positive control) and ddWater (negative control) are used as indicators to avoid false negative/positive results across all experimental procedures. The Primers/probes Mix contains multiplex primers and TaqMan probes specific to the N, E/RdRP genes of SARS-CoV-2 and RNase P gene of human, detected by FAM, VIC and ROX channels, respectively.