Bis-propargyl-PEG3 is a crosslinker with alkyne groups at both ends of the molecule. The alkyne groups react with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry to form a stable triazole linkage. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Bis-propargyl-PEG3 is a crosslinker with alkyne groups at both ends of the molecule. The alkyne groups react with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry to form a stable triazole linkage. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Attogene’s Lead Rapid Lab Lateral Flow Kit can be used for the screening of Lead in water and food samples at greater than or equal to 10 ppb in a laboratory setting. Unlike the field-based kit, the lab kit is intended for a more technical end user who will be evaluating samples in a laboratory setting.
Lead contamination is a serious worldwide environmental problem. As it is difficult to detoxify by chemical or biological methods, gradual Lead ion accumulation in the nervous and cardiovascular systems of the human body can subsequently cause serious diseases. Long-term health consequences of drinking Lead-contaminated food and water include brain, heart, kidney, lungs and immune system problems for adults, and the physical and mental development delays in infants and children. Attogene’s Lead Lateral Flow test gives results conforming of 10ppb or greater in 5-15 minutes.
This is a revolutionary product designed to make Lead testing in easy and affordable. This fast screening test kit contains 10 tests with everything needed for accurate results of unsafe lead levels. With reliable results in only 10 minutes, this test kit clearly gives results confirming Lead in water and conforming to the EPA guideline of 15 ppb (µg/L).
Sample Collection:
1. Take a first-draw sample
Immediately after opening a faucet or valve, collect a 250 mL sample. This sample should be from each tap used for consumption.
2. Take a flush sample
If first-draw sample results show elevated lead levels of 5 ppb or higher, collect a flush sample. To do this, ensure water has not been used for between 8 to 18 hours, then collect the sample at 30 seconds.
3. Take sequential samples
If you want to test a lead service line, collect 8 to 10 sequential samples, depending on how far the line is from the tap.
Standards and Regulations and recommendations for Lead
High Blood lead levels (i.e., greater than 700ppb) can cause serious health effects, including seizure, coma, and death. Blood levels as low as 100ppb have been associated with adverse effects on cognitive development, growth, and behavior among children aged 1-5 years.
Screening of Lead in water samples at 5-10 ppb
Format: 10 tests (5 tests/5 controls)
Run Time: 15 Minutes
Sample type purification kit guide
The 16S V1-V3 Library Preparation Kit for Illumina consists of the reagents and components required for library preparation of the 16S V1-V3 amplicon libraries to be used for next-generation sequencing on Illumina platforms. All molecular reagents including primers, enzyme mixes, indexes, and buffers are provided. Instructions for PCR clean up with the AMPure XP Magnetic Beads (supplied by customer) are also included for rapid purification of nucleic acid products generated at two steps of the workflow. The library prep workflow could be used for purified DNA inputs from different sources including stool, soil, water, saliva, plant, urine, skin swab, vaginal swab, cheek swab, nasal swab, plasma/serum, tongue swab, gum swab, and others.
The 16S V1-V3 Library Preparation Kit for Illumina has a streamlined procedure that reduces the handling time such that the library prep procedure can be completed in approximately 4 hours (see diagram below). Input DNA is first subjected to targeted PCR to amplify the V1-V3 region of the DNA encoding 16S rRNA. The post-PCR reaction is then cleaned up using AMPure XP beads. Dual index primers are then added using a limited-cycle PCR. The indexed amplicons flanked by 5′ and 3′ barcoded adaptors are then cleaned using AMPure XP beads. The libraries are then ready for quantification, pooling and sequencing.
Figure 1 / 3
Click for expanded view
| Minimum amount of starting material: | 2.5 µL of DNA (5 ng/µL) |
| Time to complete library preparation: | 4 hours |
Storage Conditions and Product Stability
Norgen’s 16S V1-V3 Library Prep Kit for Illumina is shipped as one kit box (for the 24 prep kit) or two sub-component kits (for the 96 prep kit). All kits should be stored at -20°C upon arrival.
All kit components should remain stable for at least 1 year when stored at the specified storage conditions.
| Step | Component | Cat. 70200 (24 preps) | Cat. 70210, 70220, 70230, 70240 (96 preps) |
|---|---|---|---|
| Amplicon PCR (PCR 1) | MGX Master Mix | 330 µL | 1,320 µL |
| 16S V1-V3 Primer Mix | 70 µL | 280 µL | |
| Index PCR (PCR 2) | Indexing Master Mix | 660 µL | 2 x 1,320 µL |
| N7xx Index Primer | 50 µL | 50 µL | |
| S5xx Index Primer | 70 µL | 70 µL | |
| PCR Clean-Up | Resuspension Buffer | 2 x 1,250 µL | 2 x 5,000 µL |
| Nuclease-free water | 1,250 µL | 1 x 6,000 µL |