Product Description
Kit Storage and term of Validity
Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.
Term of Validity: 14 months
Isothermal nucleic acid Principle Summary
This kit is based on a rapid nucleic acid amplification technology at room temperature and constant temperature: at room temperature and constant temperature (generally 39 ºC~42 ºC), reverse transcriptase uses specific primers and template RNA to synthesize cDNA strands, and binds the auxiliary protein and single strand With the help of the protein, the recombinase and the primer form a complex; perform a homology search and bind the target homology domain, at this time a D-loop region is formed at the homology position and strand exchange begins; accompanied by the recombinase from the complex Upon dissociation, the polymerase also binds to the 3′ end of the primer, initiating chain extension. Relying on the action of nfo enzyme, adding specific molecular probes designed according to the template, and using colloidal gold technology (sandwich method) can detect the final result.
Isothermal nucleic acid Product Features
1/ High sensitivity and specificity, short reaction time.
2/ The reagent form is freeze-dried, stable and easy to operate.
3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.
Technical Parameters:
| Parameters | Details |
|---|---|
| Product Name | RNA Isothermal Amplification Kit NFO |
| Manufacturer | Amp-future |
| Storage Temperature | -20°C |
| Kit Components | Enzymes, Buffers ,Reagents |
| Packaging | 48 Tests/box |
| Detection Limit | 500-1000copies/µL |
| Shipping | ICE |
| Test Time | 5-20mins |
Isothermal nucleic acid Applications
Suitable for RNA isothermal rapid amplification kit(NFO type)
Primer: Require pair of nucleotide primers with the length of 25-35 bp.
RNA NFO kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.
Notes
1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.
2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.
Interested in the analysis of DNA or RNA modifications? Then this DNA polymerase could simplify your site-specific analysis of such modifications. The 2′-O-Me sensitive DNA polymerase was engineered to catalyse DNA synthesis from both DNA and RNA and to quantify 2′-O-methylation of nucleotides site-specifically from RNA by real-time PCR. For further information refer to the original publication.
Available upon request and for R&D use only – Contact Us
The 2′-O-Me sensitive DNA polymerase is supplied as a 5 µM solution containing glycerol and is supplied together with 10x reaction buffer.
The enzyme can also be used for real-time cycling, when adding a suitable dye.
Interested in the analysis of DNA or RNA modifications? Then this DNA polymerase could simplify your site-specific analysis of such modifications. The 2′-O-Me sensitive DNA polymerase was engineered to catalyse DNA synthesis from both DNA and RNA and to quantify 2′-O-methylation of nucleotides site-specifically from RNA by real-time PCR. For further information refer to the original publication.
Discover our Tryptose Phosphate Broth, formulated to support the cultivation of fastidious microorganisms, including Haemophilus influenzae and Neisseria species. Ideal for microbiological research, pharmaceutical testing, and vaccine production.
Discover our Tryptose Phosphate Broth, formulated to support the cultivation of fastidious microorganisms, including Haemophilus influenzae and Neisseria species. Ideal for microbiological research……