This kit provides a convenient and rapid method to purify total RNA from small amounts of stool samples. All types of stool samples can be processed with this kit, including animal fecal samples, manure and samples collected using Norgen’s Stool Nucleic Acid Collection and Transport Devices Dx (Cat. Dx45660). The kit removes all traces of humic acids using rapid and simple spin column procedures. Bead tubes are also provided for effective homogenization of stool. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA and small interfering RNA. Both host and microbial RNA is recovered. The protocol does not rely on the use of phenol or chloroform, thereby providing a user friendly procedure and allowing high-throughput analysis. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR and reverse transcription PCR for gene expression analysis. The procedure can be completed in approximately 30 minutes.
NOTE: This product is not available for sale in the United States.
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Kit Specifications | |
Maximum Stool Input | 200 mg |
Type of Stool Processed | Frozen and fresh stool |
Maximum Column Binding Capacity | 50 μg |
Maximum Column Loading Volume | 650 μL |
Time to Complete 10 Purifications | 30 minutes |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
Component | Cat. Dx49500 (50 preps) |
---|---|
Lysis Solution | 60 mL |
Wash Solution | 22 mL |
Elution Buffer | 6 mL |
Bead Tubes | 50 |
Spin Columns | 50 |
Collection Tubes | 50 |
Elution Tubes (1.7 mL) | 50 |
Product Insert | 1 |
This kit provides a rapid, single column method for the isolation and purification of total RNA (including miRNA) and proteins sequentially from a single sample of cultured animal cells, tissues, blood, bacteria, yeast, fungi or plants. The total RNA and proteins are both column purified in under 25 minutes using a single column.
Purified RNA is of a high quality and yield, and is suitable for NGS, RT-qPCR and microarrays.
This kit eliminates DNA efficiently using a gDNA removal column.
Proteins are eluted in buffer and are ready for downstream applications such as Western Blots, Mass Spec and ELISA. The proteins will not require precipitation, resuspending of pellets, or any further cleaning.
This kit is ideal for researchers who are interested in studying the transcriptome and proteome of a single sample, such as for studies of microRNA profiling, gene expression including gene silencing experiments or mRNA knockdowns, studies involving biomarker discovery, and for characterization of cultured cell lines. Norgen’s RNA/Protein Purification Plus Kit is especially useful for researchers who are isolating macromolecules from precious, difficult to obtain or small samples such as biopsy materials or single foci from cell cultures, as it eliminates the need to fractionate the sample. Furthermore, analysis will be more reliable since the RNA and proteins are derived from the same sample, thereby eliminating inconsistent results. The purified macromolecules are of the highest purity and can be used in a number of different downstream applications.
Protocol
Figure 1 / 2
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Kit Specifications | |
Maximum Column Binding Capacity | 50 μg for RNA |
Maximum Column Binding Capacity | 200 μg for Protein |
Maximum Column Loading Volume | 650 μL |
Size of RNA Purified | All sizes, including small RNA (< 200 nt) |
Time to Complete 10 Purifications | 30 minutes |
Average Yields*: HEK 293 Cells (1 x 106 cells) HEK 293 Cells (1 x 106 cells) Liver (15 mg) Liver (15 mg) | 10-15 μg RNA 70-100 μg protein 30-35 μg RNA 100-150 μg protein |
* Average yields will vary depending upon a number of factors including species, growth conditions used and developmental stage.
Storage Conditions and Product Stability
The Protein Loading Dye should be stored at -20°C after the addition of DL-Dithiothreitol (DTT). All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
Component | Cat. 48200 (50 preps) |
---|---|
Buffer SKP | 40 mL |
Wash Solution A | 38 mL |
Elution Solution A | 6 mL |
Wash Solution C | 30 mL |
Binding Buffer A | 8 mL |
Elution Buffer C | 4 mL |
Protein Neutralizer | 4 mL |
Protein Loading Dye | 2 mL |
gDNA Removal Columns | 50 |
RNA/Protein Purification Columns | 50 |
Collection Tubes | 150 |
Elution Tubes (1.7 mL) | 100 |
Product Insert | 1 |
TDL5E Bench top low speed refrigerated centrifuge machine
Application:
Widely used in the field of biochemistry, biological products, pharmaceutical factory and laboratory.
TDL5E Technical Parameters:
Max. Speed | 5000rpm | Max.RCF | 4420xg |
Max. Volume | 4x180ml | Speed Accuracy | ±20rpm |
Timer | 1~99h59min | Noise | ≤60dBA |
Dimension(LxWxHmm) | 570x600x340mm | Net Weight | 76Kg |
Power Supply | AC220V/110V 50HZ/60HZ | Temperature Range | -20℃~40℃ |
Temperature Accuracy | ±1℃ | Certifications | CE, ISO & Calibration report is available. |
Matched Rotors for TDL5E:
Order No. | Rotor Type | Max. Speed(rpm) | Volume(ml) | Max.RCF(xg) |
5E-1 | Swing rotor | 4000 | 4x180ml | 2580 |
5E-2 | Microplate rotor | 4000 | 2x3x96well | 2020 |
5E-3 | Swing rotor | 5000 | 4x1x50ml | 4420 |
5E-4 | Swing rotor | 5000 | 4x1x100ml | 4420 |
5E-5 | Swing rotor | 4000 | 4x2x50ml | 2820 |
5E-6 | Swing rotor | 4000 | 4x4x15ml | 2820 |
5E-7 | Swing rotor | 4000 | 4x6x10/7ml | 2580 |
5E-8 | Swing rotor | 4000 | 4x6x5ml | 2170 |
5E-9 | Swing rotor | 4000 | 4x4x10/7ml | 2580 |
5E-10 | Swing rotor | 4000 | 4x4x5ml | 2170 |
5E-11 | Fixed rotor | 5000 | 6x15ml | 2540 |
5E-12 | Fixed rotor | 5000 | 12x15ml | 3080 |
5E-13 | Fixed rotor | 5000 | 24x15ml | 3500 |
5E-14 | Fixed rotor | 5000 | 4x50ml | 2520 |
5E-15 | Fixed rotor | 5000 | 6x50ml | 2850 |
5E-16 | Fixed rotor | 5000 | 4x100ml | 2630 |
5E-17 | Fixed rotor | 5000 | 6x100ml | 3130 |
TDL5E is a table top low speed refrigerated centrifuge widely used in the field of biochemistry, biological products, pharmaceutical factory and laboratory.
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Tel : 081-875-1869 , 02-328-7179
Email : hej@a3p-scientific.com
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